FastQCFastQC Report
Tue 26 Sept 2023
SRR8443312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8443312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17410650
Total Bases870.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA4505792.5879504785863823No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3471691.9940036701673978No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC2969211.7053987071131749No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2868311.6474456726199194No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG2676711.5373980868031922No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2355971.353177509168239No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2274391.3063211310318683No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG2260211.2981766907036785No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA2246461.2902792256463715No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2234241.2832605330645324No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1838771.056117950794485No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG1746261.0029838058889244No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1603540.9210109903995543No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG1478130.8489803654659648No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1360570.7814584751287287No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA1341900.7707351534836436No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA1271260.7301622857274139No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC1187900.6822835448418065No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA1151850.6615778273642857No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1145510.6579363780214983No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1130740.649453064647213No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA959720.5512258301671678No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT959290.5509788548962847No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG951490.5464988383546852No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA907840.5214279765545801No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA819760.47083825130020995No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG747440.42930045690425117No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA678750.3898475932834214No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT666220.38265084876210825No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660030.37909555358358243Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA656910.3773035469669426No Hit
AGCTAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA655990.37677513475947194No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG652650.3748567687019152No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG649420.37300158236481695No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG621630.3570400875326309No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA578040.3320036873982304No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA538060.30904073081705735No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA535580.30761631530126676No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG525930.302073730733775No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA457540.2627931754414683No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC457450.2627414829429114No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA438190.251679288251731No Hit
TGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA388730.2232713884892293No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG387710.22268554017225087No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC383460.22024450551817423No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC380100.21831465223871596No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG377740.21695916005433458No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG371940.21362786570288875No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA371440.21334068515535032No Hit
TCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAG358580.20595440147266184No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG349800.200911511057887No Hit
TTGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA343940.19754575504073654No Hit
AGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGA338990.19470266762010607No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG335230.19254306990261708No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC335050.19243968490550323No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA333520.19156091243003565No Hit
TGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA322580.18527740204989476No Hit
AGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC321400.18459965595770406No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG319290.18338775404709187No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG318470.18291677794912883No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG310350.1782529658571047No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC299890.1722451488026007No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC295370.16964903665285327No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC291730.16755836226677348No Hit
ACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA278600.16001700108841427No Hit
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT272220.1563525773018239No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG266530.15308446267083653No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA265430.15245266546625197No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG264310.1518093810397659No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA255370.14667459284977874No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG253370.145525870659625No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG246710.1417006257664131No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT243820.14004072220164096No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC240500.13813384336598575No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC238850.13718614755910893No Hit
AAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAG236890.1360603998127583No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA236420.13579045009807217No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT233170.13392377653907236No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT224940.12919678472658977No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG220540.12666959590825155No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT218070.1252509240034117No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG210470.12088577968082755No Hit
TAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA204540.11747981838702173No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG202230.11615304425739417No Hit
ACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC201920.11597499231792036No Hit
TGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG198910.11424616542173899No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA196590.11291364768116066No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC194690.11182236160051462No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG194630.11178789993481003No Hit
TTCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAA193210.11097230717980087No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA192970.11083446051698242No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT192790.11073107551986859No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC191520.11000163692912097No Hit
TAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCGGAAGAG189720.10896778695798262No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG185620.10661290646816748No Hit
CTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC183570.1054354662232599No Hit
CGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAGA182370.10474623290916768No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG181710.10436715458641693No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA180580.10371812654898008No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG177930.1021960696470264No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA176720.10150109272198339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)