FastQCFastQC Report
Tue 26 Sept 2023
SRR8443313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8443313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16184277
Total Bases809.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA3592192.21955543642759No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3509762.1686232878984955No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3163011.9543721353755872No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3109221.9211361743252418No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2738251.691919880016883No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2734061.6893309475610185No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC2207201.3637927724544012No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1725721.06629415697717No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA1664201.0282819553817573No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1517980.9379350093921404No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA1464860.9051130303812768No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1374600.8493428529430138No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1312040.8106880523609427No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1271650.7857317321002353No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG1254420.7750855969654993No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA1142050.7056540122243335No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1016710.6282084766591675No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG976660.6034622368364061No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG940210.5809403781213087No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG900660.5565030801190562No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA871790.5386647794028736No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA837990.5177803123364733No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG740610.4576108033741637No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG708540.4377952750067241No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC661430.4086867766783774No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA641400.3963105673487917Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG619930.3830446055761404No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG610300.37709438611313933No Hit
AGCTAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA589440.36420533336150884No Hit
ACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC564020.34849873120683733No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA558680.3451992325638025No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA546600.33773519818030795No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG539400.3332864359649801No Hit
ACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA535150.33066043049065463No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG515200.3183336518523503No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC509420.31476228440726767No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG485830.30018640931565865No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA473190.2923763600931942No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG465430.2875815830388963No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG459430.2838742811927898No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC454330.2807230746235992No Hit
TGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG450960.27864080675336933No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA438690.2710593744780814No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG425090.26265615696023986No Hit
TACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA423420.2616242912797402No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC406450.2511388058916688No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG402680.24880938456503188No Hit
AGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG401710.24821003743324463No Hit
AGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC399710.2469742701512091No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA397260.24546045523071558No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA390030.24099315650615719No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC378170.23366505652368655No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA366290.22632459886839557No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA353490.21841568826336819Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG353130.2181932501526018No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA334960.2069663043953091No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC324630.20058356638359565No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGA323270.19974324463181148No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC322990.19957023721232653No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA322560.19930454724668886No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC321040.19836536411234187No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG309370.19115466202166462No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT297500.1838203832027838No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG288710.17838918599823766No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT288490.17825325159721378No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG272170.1681693905758039No Hit
TCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAGATCGGAAG266270.16452387709379912No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA266010.16436322734713452No Hit
TTGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC264420.16338079235791628No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC262590.16225006529485378No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA252500.15601561935698455No Hit
TCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC248000.15323514297240465No Hit
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT247220.15275319373241078No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA246840.15251839794882405No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG244410.15101694070115088No Hit
CCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC239900.1482302854801608No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT239050.14770508438529567No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG236220.14595647368121542No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC225850.13954902032386124No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT223960.13838122024233768No Hit
TTGCGTGCATTTATCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA221040.13657700001056583No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT220260.13609505077057196No Hit
TACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAG217240.1342290421746983No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG210850.13028076570859484No Hit
TCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC210030.12977410112296026No Hit
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC201560.12454062668353982Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC197440.12199494608254667No Hit
TACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG194170.11997446657641857No Hit
TCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT192710.11907235646053266No Hit
TAGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT190490.11770065477747323No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC189970.11737935528414399No Hit
TGTGCATTTATCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT189330.11698390975389261No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG185390.11454944820828265No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC181690.11226327873651692No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA180060.11125612840165798No Hit
TGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGGA179090.11065678126987075No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG177610.10974231348116446No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA176810.10924800656835025No Hit
TCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGA173920.10746232284580892No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA173850.10741907099093767No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG173370.10712248684324917No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG170980.10564574494121672No Hit
TAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA167380.10342136383355278No Hit
CGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA165060.10198787378639157No Hit
CCCGCAACGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG163750.1011784462166583No Hit
CCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGC162520.10041844933820646No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)