FastQCFastQC Report
Mon 17 Jul 2023
SRR8449566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104449069
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC31013612.9692567197511353No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC25275122.419851152526788No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA24462992.3420974676184048No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC17491651.6746582968585388Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG10748461.0290623078698766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT10151730.9719311141011702No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT10061990.963339366864055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT9599280.9190393070904251No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG9389130.8989194532696122No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA9091600.8704337996540688No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG8497820.8135850401883429No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT8088130.7743611386330308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT7869430.7534227040357823No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT7831800.7498199912150486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT7729430.7400190421994092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC7007690.6709193358152383No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC6508410.6231180480890643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT6203660.5939411484845307Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC6173910.5910928703443015No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG5966370.5712228990762952TruSeq Adapter, Index 8 (100% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT5953500.5699907195917658No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC5914150.5662233332113281No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC5902710.5651280625584131No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC5372510.5143664803752344No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT5153080.49335815525555327No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAGAGCACACGT4749280.4546981649017858No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG4412340.42243938047930324No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA4310750.4127131090081808No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC3760800.36006065310165664No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG3716040.3557753109316848No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC3609690.3455933149581257No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAGATCGGAAGAGCACACGTC3488800.33401925296241747No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA3455590.3308397128939464No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT3358760.32156916592526064No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT3329590.31877641724121064No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG3282010.3142210870256775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3239650.3101655219157578No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG3142250.30084040289530967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT3132530.29990980580209864No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC3081870.29505959502616536No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG3052040.29220365765060097No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2957380.283140867440379No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2939560.2814347727694921Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACA2900100.27765685494046866No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2893400.2770153939811565Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC2868420.2746237977477808No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2858780.2737008598899048Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG2834910.2714155355468032No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACA2802240.2682876953168439No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC2769380.2651416644029637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT2585210.2475091472572149No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2541770.24335018247027168No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG2537330.2429250949091753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC2470860.23656122775014876Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2447180.2342940940909679No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC2364710.22639837986492728No Hit
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA2363640.22629593759232072Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT2339120.22394838196212163No Hit
CTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAAC2321680.2222786686590763Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG2283930.21866446698533998No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG2260810.21645094797350467No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC2154940.20631490741195596No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC2119830.20295346050427698Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT2088700.19997306055451772No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC2074690.19863173696646352No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT2066590.19785623938878763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT2024310.19380833351420299No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCAGATCGGAAGAGCA1971180.1887216438472994No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1970210.18862877561886165Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG1961600.18780445041592472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC1957290.18739180911224781Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA1810580.1733457289121457Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA1802450.17256735912121918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC1758060.16831744091467202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1742430.16682101781108266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAACTCC1722600.1649224848524021Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA1681850.16102106185360063Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1664820.15939060213164752Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1664470.1593570929770566No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1660660.15899232189422388No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACACG1576700.15095395441006756No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC1547860.1481928000717747No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC1526600.14615735828147977No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC1519250.14545366603507015Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGAGATCGGAAGAGCACAC1479170.14161638913219993No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAG1468290.14057473312663035No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTC1462210.139992631241165No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC1420720.13602036031551418No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1405550.13456797781510144Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA1403210.13434394518155063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1395130.13357036241270853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC1384880.13258902288540264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG1376230.13176086806479817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1363580.13054975147744016No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1362890.13048369057267517Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA1333700.1276890270797914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT1265480.12115761414781016No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1236560.11838880057418223No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA1234580.11819923449963926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAGAGCACACGTC1222820.11707332690538391No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1218430.11665302636637191No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1210910.11593305824487532No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1205990.1154620152717685No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1204970.11536436002124632Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCAGATCGGAAGAGCACACGT1191710.11409484176445842No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1173130.11231598435788835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC1170200.11203546486374139No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGAGATCGGAAGAGCACACGTCTG1154650.11054670099548708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1124090.10762087309749023No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT1118100.10704738785177682No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA1107990.10607945198630733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA1083510.1037357259737758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA1067010.10215600868591754No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1056270.10112775634218434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)