FastQCFastQC Report
Fri 14 Jul 2023
SRR8449567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153726961
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG78103005.080631236832946No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT48047403.125502493996483No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT36124122.349888384250307Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT25476821.6572772813742154No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG17972361.1691091714224418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG17961311.1683903645242815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC16319951.0616192432243554Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC15584171.0137564613665913No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12148560.7902686634129195No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG10680350.6947610185307703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA10338800.6725430550858285No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC8381400.5452134059945413Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA8267960.5378340888427502Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA8249520.536634559503196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT8209780.5340494566857403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA7438500.4838773856981404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA7157550.46560147637342547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC6165760.40108514211765367No Hit
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA6064470.39449618730184877Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT5589150.36357643211329727Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC4823250.31375433226706406No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT4727060.30749713448117927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG4186440.2723295883016903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT4069520.2647238957647774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA4013120.26105505331624945No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG3722390.24214295109886416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC3695870.24041781454327976No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3695180.24037292976864352No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3683420.2396079370878866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG3518900.22890584560505298No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT3429340.22307993195806428No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3331460.21671279899951965Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTTGGGGAGATCGGAAGAGCACACGTCT3297330.21449262891497606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC3100300.20167574899239699Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT3042040.19788591280354523No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC3011590.1959051281837283Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2988000.19437058929435286Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA2965640.1929160623945464Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC2953110.19210098090731137No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTC2825990.1838317743105583No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC2811400.1828826890033948No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA2760630.1795800802957394Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT2746540.17866352018758763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC2300510.1496490911571458Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2291660.14907339513463744Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTCT2280520.14834873370065516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2260680.147058133803868No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2223600.14464606504515498No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2135390.13890796943549805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT2064250.13428028412010304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT2041200.1327808724456603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACACG2021550.13150263212449767No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG2015880.13113379636770417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1994920.12977034002513066No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1972560.1283158131253242Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAAC1952080.126983580973802Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA1891160.12302071072620763Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1884990.12261934977040234No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT1844820.12000627528179653No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1804210.11736457861806038Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTTGGGGAGATCGGAAGAGCACACGTCTG1770130.11514766105341795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA1766800.11493104322799955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG1764950.11481069999165598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA1752300.11398781245665815Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC1748200.11372110582476161No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC1737360.11301595951018638Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1720150.11189644216020116Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT1706960.1110384274102706Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1680910.10934386454175725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1664170.10825492087884311No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG1655380.10768312788021614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1613840.10498093434631807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTG1611310.10481635683931852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC1550780.10087885624695332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)