FastQCFastQC Report
Mon 17 Jul 2023
SRR8449568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64728104
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC45929427.095746231034358No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC30801974.758670206066904No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT14533432.2453044507529527No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT11592281.7909191346003277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT8316131.2847788651433387No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC7616841.1767438761994327No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT6412310.9906531481286707No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG4643050.7173159281785852No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC4078690.6301265984864935No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA3711890.5734587869281634No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC3316870.5124311999004327No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3024610.46727925168331824No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2744650.42402755996066255No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA2616340.4042046403831016No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2584000.39920835623425643No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC2519410.38922969225237924No Hit
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC2478770.3829511212007693No Hit
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCA2454710.3792340341067305Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2341630.3617640337495441Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT2244520.34676127698719555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACG2185720.3376771239892953No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1965880.3037135152297988Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA1951860.3015475318109117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1864490.2880495310043378No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1824980.2819455363623813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGAACAAGTTACCCTAGGGATAAGATCGGAAGAGCACACGTCTGAAC1657700.2561020480377426Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA1611260.2489274210781765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1516300.2342568229713634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACG1432790.22135516282077414No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1399980.21628626724490493Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG1398260.21602054032047657No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1252530.1935063631710887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC1214880.18768972438926992No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1210040.1869419811833203No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT1207150.18649549815332148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT1137290.17570265923438758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT1131100.17474635129124128No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT1129180.1744497258872282No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG1124460.1737205217690294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1082620.16725655983991128Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC1081230.16704181540679763No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC1064480.16445406774157945No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT1053370.16273765720064967No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1049720.16217375994822897No Hit
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC1048410.16197137490694924Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1027230.15869922591893007No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC1009520.15596316555170534No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG959310.1482061022519677No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT927710.14332414247758593No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC906810.14009525136098533No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACAC891930.13779640447988403No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT882510.1363410860914449No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA865100.13365137344359723Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT864690.1335880315604486Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA838600.12955732489862518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACGTCTGAACTCC836440.12922362131911047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC831310.12843107531776307No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG754120.11650580712205011Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG732770.11320739442638393No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG721270.111430731850264TruSeq Adapter, Index 1 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC682110.10538080954758076Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT677790.10471340238855135No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG657870.10163591382191575No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA652180.10075685207773118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)