FastQCFastQC Report
Fri 14 Jul 2023
SRR8449569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53308725
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC876219116.436692117472326No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT42241067.923854866159338No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT40302677.560238966510642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT17411103.266088243528616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT16915743.173165368333233No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC15360652.881451394682578No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT9184901.7229637362364227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG8403091.5763066927599563TruSeq Adapter, Index 7 (100% over 49bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC6943341.3024772211303122No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG6321741.1858734194074234Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG6045571.1340676408974328No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC5441531.020757859055905No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4270180.8010283494868804No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3175310.5956454595378149No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2764850.5186486827437722No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT2008260.37672257214930577No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1951670.36610704908061487No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1781740.3342304660259648No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1762640.3306475628520472No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1704170.31967937706257277No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1694730.31790855999651085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1690280.3170737998329542No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1670430.3133502067438304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1624290.3046949631603457No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC1521250.2853660446765515No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG1498460.28109094711981947No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT1479790.2775887061639534No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1472470.27621557259154106No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT1325130.24857656978290138Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1294250.2427838970074786No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1119290.20996375358817904No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1116480.20943663537253987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1078750.20235899470490057No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC991620.18601457828901366No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC980380.18390610542645694No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC978310.18351780126048786No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC921490.17285913328446703No Hit
CAGTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC865220.16230363791293076No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGATCGGAAGAGCA823700.1545150442071162No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTC801280.1503093536752192No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATCGGAAGAGCAC773160.1450344197877552No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT761720.14288842961447681Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC751430.1409581639778479No Hit
CCTCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC750980.14087375002872418No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT742850.1393486713478891Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA724660.13593647193775502No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACAC723310.13568323009038388No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA715910.13429508959368283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT675480.12671096523130126No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT652310.12236458478419808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC618680.11605604898635262No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC613520.11508810236973405No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA610530.11452721857444535No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT593990.1114245369777649No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTG571540.10721321884925968Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA561190.10527169801941426No Hit
TACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC560060.10505972521383695No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT539520.10120669740272348No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTG538340.10098534526946573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT538190.1009572072864245No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGT537600.10084653121979563No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAGATCGGAAGAGC535460.10044509599507398No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)