FastQCFastQC Report
Fri 14 Jul 2023
SRR8449572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132296016
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT50701473.832426064893746No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC47973043.626189317749372No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG40273943.044229238165418TruSeq Adapter, Index 8 (100% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC29812252.2534503230996767No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT24410631.8451523135813854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC21095421.5945620010204993No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC18385691.3897387507118883No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT17427931.3173435245396958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC16277151.2303582898520542No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT13058830.9870917050140043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG10897180.8236967619644722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT10585460.8001344500049041No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT9228450.6975606884488494No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC8945130.6761450775660546No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG8742390.6608203530482732No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA8688410.6567401092410825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA8672220.6555163384511896No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG8038120.6075859457476028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT7164550.5415544788589854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6972730.5270551760228366No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC6190810.4679513553907776No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC6098080.4609420740228489No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA6056090.4577681311280001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTG5721910.43250811120419524Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA5602770.42350254901099965Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA4854580.3669483138479393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4807500.36338962769672517Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG4688460.35439162431013793No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC4673520.35326233860284956No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG4458760.33702904553074375No Hit
CGATCCCGGAGAAGCCGGCGGGAGCCCCGGGAGATCGGAAGAGCACACGT4425750.33453388346932533No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAA3156110.23856425124699146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCAGATCGGAAGAGC3039580.22975597390627397No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2903850.21949640569675202No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCAGATCGGAAGAGCACACGTCTGA2835500.2143299613799406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2775770.20981508619276942No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACAGATCGGAAGAG2735640.20678173710083603No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC2725190.20599184181026284No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCAGATCGGAAGAGCAC2597330.19632715168081855No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT2502080.18912738838635929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCTG2487900.18805554960929435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT2472210.18686957285244327No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC2292780.1733068061550697No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG2290140.17310725366060908No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG2164760.16363002193505205No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC2138400.16163752051308938No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2136630.16150372963612145No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG2099220.1586759800839354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC2098940.15865481542543203No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT2070600.15651264963262385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGCAGATCGGAAGAGCACACGT2012850.15214743881629814No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1998610.15107106475526821Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT1952610.14759401371542435No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1905100.1440028246957943No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGT1861390.14069887032728182No Hit
GATCCCGGAGAAGCCGGCGGGAGCCCCGGGAGATCGGAAGAGCACACGTC1765030.13341520427946976No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT1752680.13248169166333776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1701030.12857756805012177Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCAGATCGGAAGAG1684970.12736362370881976No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT1673560.12650116387480634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA1667920.12607484718209502Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGATCGGAAGAGCACAC1664190.12579290369560336No Hit
AAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAAC1663160.12571504798753727Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTG1649300.12466739739161913Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCAGATCGGAAGAGC1649150.12465605918170657No Hit
CGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGATCGGAAGAGCACACG1646210.1244338302674209No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA1643370.12421916015974359Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT1623930.12274972815507913No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC1606130.12140426057879172No Hit
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1598940.12086078238365092Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACAC1565590.11833992037976412No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG1511570.1142566530499301Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCAC1504270.11370486016752009No Hit
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCA1498780.11328988168472134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCAGATCGGAAGAGCAC1482660.1120714020594543No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1471240.11120818634478002No Hit
CGCCACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAAC1465020.11073802857374028Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1439980.10884530339900789No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCT1426720.10784300564273984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC1420170.10734790380989251No Hit
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC1418590.10722847466548048Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG1406260.10629647381067016TruSeq Adapter, Index 8 (100% over 50bp)
TAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACA1374160.10387009688938781No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT1360970.10287309029774563No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1360920.10286931089444144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1359660.10277406993117616No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGTCTGA1355250.10244072655974765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG1349400.10199853637315882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)