FastQCFastQC Report
Fri 14 Jul 2023
SRR8449575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105106917
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC100370629.549382939278868No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC39385183.7471539575268866Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA21809512.074983323885335No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT16651531.5842468293499656No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT16314681.552198510398702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT15935031.5160781473592266No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC14769591.4051967674020922Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG13923421.32469112380111TruSeq Adapter, Index 6 (100% over 49bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG12424861.1821163016321752No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG11460921.0904058769034202No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT10666691.0148418681141604No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC9238410.8789535706769898Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC9091620.8649877914314621No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT9091410.864967811775889No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTC8489720.8077222929105607No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT8241500.7841063400232736No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC8031980.7641723522344396No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA7083570.6739394706059164No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC6703670.6377953222621875No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC6402010.6090950227376568No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC5473950.5207982648753745No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT5417710.5154475228304908Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAGATCGGAAGAGCACACG5118060.4869384571521587No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC4750230.45194266329779226No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT4681810.4454331012296746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG4667730.4440935128941133No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC4406890.41927687784810586Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT4389550.41762712914507805No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC4322610.4112583760781415No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4318880.4109034993386782No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG4214700.40099168735012936No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC4059080.38618581115836553Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3974040.37809500206347024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCT3902750.37131238470252154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG3833130.36468865317398663No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC3502940.3332739747280381No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC3328850.3167108402580203No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG3298570.31382996420682757No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG3282010.31225442565307093No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA3133600.2981345176359801Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC3105420.29545343814051744Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2895970.27552611023687434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC2846210.2707918832782433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2618400.24911776263021773No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT2457380.23379812386657675No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC2363380.22485484946723344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2330080.22168664694065757Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA2153510.20488756225244434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2122430.2019305732276402No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC2085930.19845791880661862No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC2065710.19653416339858965No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACACGTC2043380.19440966002266052No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAGATCGGAAGAGCACACGT2028970.1930386750854846No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT1965940.1870419241770739No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1961510.1866204485857006Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1915860.18227725202899825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGCAGATCGGAAGAGCACA1894640.18025835540395502No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACACG1889710.17978930920407454No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTAGATCGGAAGAGCACA1785700.16989367122241822No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1758100.1672677736328238No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC1633080.15537321868169723No Hit
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT1618640.15399937950801088Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG1584350.15073698717659084No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1569600.14933365422563008No Hit
TAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGAACT1569540.14932794575260921Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT1552180.14767629422524114No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1530190.1455841388630969Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1474670.1403018984944635Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1450410.1379937725696968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG1435560.1365809254970346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC1422010.13529176200649098No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCACA1419670.13506913155867753No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACG1418410.13494925362523952No Hit
GTGTAATGGTTAGCACTCTGGACTAGATCGGAAGAGCACACGTCTGAACT1404290.13360585964099775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTATTAGATCGGAAGAGCACAC1394890.1327115322010634No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1360500.12943962574794196No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1330390.1265749237036417No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCAGATCGGAAGAGCACACGT1322970.12586897587339566No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG1319760.1255635725667798No Hit
TGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAAC1319340.1255236132556338Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG1315000.12511070037379177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1310910.12472157279620331No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1293620.12307658115402623No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACAC1288420.12258184682555193No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA1258920.11977518092363036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAG1258610.11974568714635593No Hit
GTCTAGGGGTATGATTCTCGGTAGATCGGAAGAGCACACGTCTGAACTCC1234820.11748227759358597Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1228430.11687432521686465Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAGATCGGAAGAGCACAC1219550.11602947120977775No Hit
GCGCGCGGGTCGGGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAACTC1209020.11502763419461727Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG1188550.11308009348233476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCA1187820.11301064039391433No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACG1171120.11142178206977567No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1155810.1099651700372869Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG1138200.10828973320566523No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT1132810.1077769220459582No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1125070.10704052902626761Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1111220.10572282317061969No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1085530.10327864530552257No Hit
TCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC1059530.10080497366315101Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1058670.10072315221651873No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC1055030.10037683818658671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)