FastQCFastQC Report
Fri 14 Jul 2023
SRR8449579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38090984
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC25230636.623780052518464No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC21629995.678506493820165No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT15308084.0188197816050115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC11795473.0966566786513052No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT11139162.9243560628415373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT10977992.882044212877252No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT9297832.440952956216621No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC9073422.3820387522674658No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6849691.7982444349560516No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT6668881.7507765092127838No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6133901.610328575392014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5242871.3764070783784426No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG4840331.2707285272546385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG4149461.089354898261489No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3382810.8880867976526938No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3297140.8655959110953921TruSeq Adapter, Index 8 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA3102050.8143790667103795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC2926460.7682815440000185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2553850.6704604953235127No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2168520.5693000737392344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2125570.5580244395891689No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1966170.5161772665153518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT1735550.45563275550980775No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC1634890.4292065544959406No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1594230.41853211248100075No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT1550670.40709633544777946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1190620.31257265498838255Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG1181240.3101101299982169Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC1160600.3046915249025859No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1122770.2947600408537622No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1032800.27114027823487047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1009780.26509685336561534No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGA1008990.2648894552054628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA908940.23862339707475133Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG833570.21883656247893205No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGA802560.21069552836965305Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA797360.20933037592307932Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG792850.2081463687049933No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT685480.1799585959764127No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG668690.1755507287498795No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA653570.1715812854821498No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAACTAGATCGGAAGAGCACACGTC650830.17086195515453212No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG624770.16402044116266462No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC606600.15925028347915612No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA595750.15640184039351673No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG564270.14813741750541282No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG539470.14162669045252285No Hit
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC536800.14092573717707058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA525580.13798015824427115No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT520060.13653099641636982No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA516430.1355780149969347No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA513940.13492431699847923No Hit
ACTTTGCAAGGAGAACCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG512520.13455152536883794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCATTTATCAGTTGGTGACTCTAGATAACCTAGATCGGAAGAGCACACG508890.13359854394940282No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTG500140.13130141242872592Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC499620.13116489718406854No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT498890.1309732507829149No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG493300.1295057119028482No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC469150.1231656289057799No Hit
GAAATAAGGCCTACTTCACAGATCGGAAGAGCACACGTCTGAACTCCAGT454170.11923293974238103Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT437180.11477256665251807No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC422010.11078999691895594No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT420630.11042770646198061No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA411970.10815420257980209Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT411070.10791792619481817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC400800.1052217501128351No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCAC397310.10430552279773082No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT397120.10425564222756756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGCTAAGACTTCACCAGTCAAAGCGAACTAGATCGGAAGAGCACACGT397050.10423726517540213No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTG390470.1025098222718531Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT389760.10232342645703246No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTG389430.10223679178253836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)