FastQCFastQC Report
Fri 14 Jul 2023
SRR8449580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8449580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39566395
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC25530246.4525059712920525No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT25168236.361011661537525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC23387405.910925167683334No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT15403973.893195222865262Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT11864042.998514269495616No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT10090512.5502727756723855No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC9886572.498729035081412No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC7710351.9487117792763278No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT7526291.9021925045230936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6437741.6270726711392334No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG5317501.3439435157031618No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC4259871.076638394779206No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3747620.9471724679491269No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG3123090.7893289242044922Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG3048960.7705933279996826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2816230.7117732105742766No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2800310.7077495940684007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2613030.6604164973837015No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC2396980.6058120786591753No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2229300.5634326806877402No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2077920.525172940319683No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT1863190.47090213803911124No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1806160.4564883912218942No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG1787000.4516458979899483No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACAC1633160.4127644178854303No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1590580.4020027601705943No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA1495540.37798237620586866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT1086730.2746598470747714No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA993000.2509705521566976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC930010.2350504765470799No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC896160.22649523667748855No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT854270.21590796937653783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTG839040.2120587432845474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA824430.20836621582532347No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG817110.20651616099975748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG761250.19239811966695475No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC689100.17416294812807687No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA660490.16693206444509284No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA631730.15966326980256856No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT603980.15264974228761555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGAGATCGGAAGAGCA599680.15156296144746065No Hit
CTTTCCGCCTAGGGGAAAGTCCCCGGACCTAGATCGGAAGAGCACACGTC559180.14132700237158324No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC546320.13807676944032934No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTC517490.13079028301668627No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT478140.12084497463061773No Hit
CGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGATCGGAAGAGCACACG474970.12004378968566633No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG451130.11401847451606344No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG434570.1098331045828158No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG433340.10952223471458544TruSeq Adapter, Index 2 (100% over 49bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT433320.10951717991998008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT430110.10870588538581794No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC429600.10857698812338096No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG415700.10506390587264773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)