FastQCFastQC Report
Fri 14 Jul 2023
SRR8494660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65940070
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length8-42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAC21746153.2978657741794932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13733882.082782138387175No Hit
AGCAGTGGCTGGTTGAGATTT11291791.7124322130686245No Hit
GGGAGAGATCC10345631.5689443459796146No Hit
ATGTACACGGAGTCGAGCTCAACCC6941191.0526512938187662No Hit
GGGAGAAAGCCC6384610.9682443467227134No Hit
CAGTGGCTGGTTGAGAT5632300.8541543859446918No Hit
TGGGAGAGATCC5215370.7909257603153894No Hit
TAGCAGTGGCTGGTTGAGATTT4998090.7579746275671227No Hit
AGAGCAGTGGCTGGTT4912370.7449749446732464No Hit
GGGGAGAGATCC4607040.6986707778745155No Hit
TAGAGCAGTGGCTGGTT4349460.6596080349929868No Hit
TGGGAGAAAGCC3378900.5124198381955009No Hit
GGGAGAAAGCC2899600.43973262388104833No Hit
TGAATACAAGCTTGGGCTGC2623200.39781577423257214No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTG2418790.36681641375266966No Hit
GGGGAGAAAGCC2193560.33265964079201005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1939280.29409735233826717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1856640.2815647602436576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1836230.2784695254342314No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG1832920.27796755447787663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1810890.27462664204026477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1800860.27310556388551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1763620.26745801149437665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1734090.262979702629979No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1731840.26263848370194326No Hit
GGGAGAAAGCCCC1720030.2608474634618981No Hit
AAAAAAAAAAAAAAAAAAAAAAA1703690.25836945578007425No Hit
ATGTACACGGAGTCGACCCAACGC1647830.24989812719337423No Hit
GGGGAGAAAGCCC1639390.248618177081098No Hit
AAAAAAAAAAAAAAAAAAAAAA1629710.24715017742625994No Hit
AAAAAAAAAAAAAAAAAAAAA1577110.23917323715306943No Hit
TGGGAGAAAGCCC1554640.23576559745841943No Hit
ATGTACACGGAGTCGACCC1517080.23006951615307658No Hit
AAAAAAAAAAAAAAAAAAAA1469420.2228417409929956No Hit
AAAAAAAAAAAAAAAAAAA1453780.22046989031100514No Hit
TACACGGAGTCGACCCAACGCGA1394060.21141318169665274No Hit
AAAAAAAAAAAAAAAAAA1378520.20905649630035272No Hit
TAGAGCAGTGGCTGGTTGAGATTT1377660.2089260748434146No Hit
AGAGCAGTGGCTGGTTGAGATTT1375270.20856362451541224No Hit
AAAAAAAAAAAAAAAAA1370570.20785085608795986No Hit
AGGGAGAGATCC1350160.2047556212785337No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG1261260.19127368229970032No Hit
ACGGAGTCGACCCAACGCGA1216970.1845569772673884No Hit
AAAAAAAAAAAAAAAA1200390.18204257289990744No Hit
TGAATACAAGCTTGGGCTGCAGGTC1180910.17908837524740268No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGG1110140.16835590256425265No Hit
AAAAAAAAAAAAAAA1108320.16807989436468598No Hit
AGATCGGAAGAGCGGTTCAGAA1095250.16609779152494075Illumina Paired End PCR Primer 2 (95% over 22bp)
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGG1090640.1653986718546098No Hit
TAGAGCAGTGGCTGGT1056210.16017726399137885No Hit
ATGTACACGGAGTCGA1031600.15644508718295264No Hit
AAAAAAAAAAAAAA1007480.15278722027440977No Hit
AAGATCTGCACCTGCGGCGGCTCCACCCGGGCC982250.14896101869470263No Hit
TGGGAGAAAGCCCC961280.14578085828540976No Hit
ATGTACACGGAGTCGAGCTC946700.14356975963173835No Hit
TGAATACAAGCTTGGGCTGCAG940870.14268562347598357No Hit
AAAAAAAAAAAAA940170.14257946647615025No Hit
TCAGTGGCTGGTTGAGAT910700.1381102567831669No Hit
AAAAAAAAAAAA897800.13615393492909547No Hit
AGATCGGAAGAGCGGTTCAAA884450.13412936928941688No Hit
TGTACACGGAGTCGAC864990.1311782046940502No Hit
AGCAGTGGCTGGTTGAGATT848920.12874114328359068No Hit
AAAAAAAAAAA846430.12836352766989784No Hit
GGGAGAAAGCCTACAGC797270.12090827322445973No Hit
CAGAGCAGTGGCTGGTT788910.1196404553407359No Hit
AGAGCAGTGGCTGGTTGAGATTTA780850.11841813331408353No Hit
ACGGAGTCGAGCTCAACCC772580.11716396418748115No Hit
TATGTACACGGAGTCGAC771020.11692738573070972No Hit
GGAAAAAAAAAAAAAAA754500.11442208053464305No Hit
AAAAAAAAAA723750.10975875518482162No Hit
GGAAAAAAAAAAAAAA703710.10671963193245018No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG698800.1059750164050478No Hit
GGAAAAAAAAAAAAAAAA695350.10545181404872637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)