FastQCFastQC Report
Tue 11 Jul 2023
SRR8494662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40328556
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length8-42
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2588990.6419743865860211No Hit
TGAATACAAGCTTGGGCTGCAGGTC2128370.5277575522416424No Hit
AAGATCTGCACCTGCGGCGGCTCCACCCGGGCC1986200.49250461633191134No Hit
ATCAGTAGGGTAAAACTAACCTGTCTCACGACG1490440.3695743532200855No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG1405390.3484850784144119No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAC1219510.3023936686451159No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTG1158400.2872406341551133No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTT1111580.2756309945736713No Hit
AGTAGGGTAAAACTAACCTGTCTCACGACGGTC1063750.26377091210506026No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGG1028730.2550872389281679No Hit
TGAATACAAGCTTGGGCTGC1012690.25110990832401736No Hit
AGGAAGAGCCGACATCGAAGGATCAAAAAGC977110.2422873757245362No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTC953900.2365321485847398No Hit
GGGAGAGCGACGCTCAGAC945150.23436247010678984No Hit
ACTTTGACATTCAGAGCACTGG908710.22532668910833306No Hit
AAAAGGGCAGGGACTTAATCAACGCAAGCTT858680.2129210874795517No Hit
TCCGCCGAGGGCGCACCACCGGC858580.21289629115408942No Hit
CCGCCGAGGGCGCACCACCGGC854130.21179285467101774No Hit
AAAAAAGCGACGTCGCTATGAACGCTTGGCCG851150.2110539241722416No Hit
ACGTACGAAACCCCGACCCAGAAGCAG848720.21045137346350806No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG844440.20939009073372228No Hit
AAAAAGTGGCCCACTAGGCACTCGCATTCCAC818900.2030571092106546No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGG813910.20181977257008654No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCG813640.2017528224913384No Hit
TCGACGAAGCCGAGCGCACGGGGT811120.20112795508968878No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGG790220.19594552306807117No Hit
ACCAAGATCTGCACCTGCGGCGGCTCCACCCGG788550.19553142443285101No Hit
AAAATGTCTGAACCTGCGGTTCCTCTCG779730.19334438852707744No Hit
ATGGCCTCAGTTCCGAAAAC771530.19131108983917006No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCC769970.1909242671619584No Hit
ATCGAAGGATCAAAAAGCGACGTCGCT769120.19071349839552895No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGG758420.18806029157106444No Hit
ATTGAATTTCTTCACTTTGACATTCAGA721560.17892036600566608No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG611780.15169895991316923No Hit
ACTGAGCAGGATTACCATGGCAA610820.15146091518873128No Hit
ACGAAACCCCGACCCAGAAGC603240.14958135371869005No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAC595900.1477613034297583No Hit
GGGAGAAAGCCTACAGCACCCGGT591960.14678432820654427No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG591060.1465611612773837No Hit
AAATGATAGGAAGAGCC590370.14639006663169393No Hit
AGAAGCGAGAGCCCCTCGGGGCTCGC586000.14530646720899207No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG585040.14506842248455412No Hit
AGATGGTAGCTTCGCCCCATTGGCTCCT572180.1418796150301042No Hit
AAATGTCTGAACCTGCGGTTCCTCTCGTACTG538160.13344390510783474No Hit
ATCCCGCAGCGCCAGTTCTGCTTACCAAAAG535400.1327595265250757No Hit
GGGAGAAAGCCTACAGC523630.12984099901816468No Hit
TGGGAGAAAGCCTACA513470.1273216923511965No Hit
ACATACACCAAATGTCTGAACCTGCGGTTC512060.1269720641621783No Hit
ATTGCGCCACGGCGGCTTTCGTGCGAGCCCCC507750.1259033425347538No Hit
ACGACGGTATCTGATCGTCTTCGAACC507150.12575456458198006No Hit
AGGACACCTGCGTTACCGTTTGACAGGTG499820.12393699392559455No Hit
AACGCTTGGTGAATTCT495520.1228707519307163No Hit
CCGCCGAGGGCGCACCACCGG487470.12087464773100232No Hit
ACTGAGCAGGATTACCATGGCAACAA473160.11732629355734928No Hit
ATCCCGAAGTTACGGATCCGGCTTGCCGACTT468900.11626997009265592No Hit
TCCGCCGAGGGCGCACCACCGG462640.11471772011871688No Hit
AAGTACTAACCAGGCCCGACCCTGCT449800.11153387192935943No Hit
ATTCTACACCTCTCATGTCTCTTCACCG446990.11083709518386921No Hit
AAATGATAGGAAGAGCCGACAT442580.10974357723098242No Hit
ATGCCAGAGTCTCGTTCGTTATCGGAAT433050.10738048741442663No Hit
TAGGGTAAAACTAACCTGTCTCACGACGGTC432290.10719203534091327No Hit
TCAAAAAGTAAGTTTAGATTATAGC431940.10710524820179528No Hit
AAAAAAAAAAAAAAAAAAAAAAAA423550.10502483649550953No Hit
ACATCATCAGTAGGGTAAAACTAACCTGTCTCA420820.10434789681038914No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA415330.10298657854250967No Hit
AAAAAAAAAAAAAAAAAAAAAAA404910.10040280142933956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)