FastQCFastQC Report
Tue 11 Jul 2023
SRR8494664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44110344
Total Bases784.7 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGAGATCC8370631.8976569305376536No Hit
TGGGAGAGATCC5701051.2924519473255525No Hit
GGGGAGAGATCC5118421.1603672825584856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490721.0180650597510643No Hit
GGGAGAAAGCCC4084580.9259914182487446No Hit
TGGGAGAAAGCC2179480.4940972575502925No Hit
GGGGAGAAAGCC1491850.33820865237414605No Hit
AAAAAAAAAAAAAAAAAAAAAAA1461180.3312556347327511No Hit
AAAAAAAAAAAAAAAAAAAAA1369350.31043738856355324No Hit
AAAAAAAAAAAAAAAAAAAAAA1357660.3077872165313424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1356350.3074902340367148No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1352710.3066650307692001No Hit
GGGGAGAAAGCCC1337450.30320552476308055No Hit
GGGAGAAAGCC1327370.3009203464838089No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1283040.29087054954728986No Hit
AAAAAAAAAAAAAAAAAAAA1274680.2889753024823384No Hit
AAAAAAAAAAAAAAAAAAA1250990.28360468011766127No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1227190.2782091202916033No Hit
AAAAAAAAAAAAAAAAA1204040.27296091819188717No Hit
GGGAGAAAGCCCC1193570.27058732527681034No Hit
AAAAAAAAAAAAAAAAAA1176530.2667242858047083No Hit
TGGGAGAAAGCCC1175650.26652478611366076No Hit
AAAAAAAAAAAAAAAA1109610.25155324111732164No Hit
AGGGAGAGATCC1104700.25044012352295414No Hit
AAAAAAAAAAAAAAA1085710.24613501087182632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1079570.24474304711838113No Hit
AAAAAAAAAAAAAA1014090.22989845647089038No Hit
AAAAAAAAAAAAA972880.22055597662081258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA963620.21845669578092614No Hit
AAAAAAAAAAAA929350.2106875430397913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860870.19516283980918397No Hit
AAAAAAAAAAA857310.194355772877219No Hit
TGCCGTCGGGTCCCGGGG844880.19153783974117272No Hit
TGGGAGAAAGCCCC818750.1856140591422275No Hit
GGGAGAGCGACGCTC805900.1827009102445449No Hit
GGAAAAAAAAAAAAAAA789340.1789466887857415No Hit
GGAAAAAAAAAAAAAA744730.1688334146747983No Hit
GGAAAAAAAAAAAAAAAA731940.16593386802877802No Hit
TGAATACAAGCTTGGGCTGCAGGTC720960.16344465597457142No Hit
GGAAAAAAAAAAAAAAAAA718480.16288242957252838No Hit
ACAAGCTTGGGCTGCAGGTC716490.16243128822572775No Hit
AAAAAAAAAA712880.16161288608404414No Hit
GGAAAAAAAAAAAAA712520.16153127257407016No Hit
GGGAGAGCGACGCTCAGAC711970.16140658526716545No Hit
TGAATACAAGCTTGGGCTGC673400.15266260449023022No Hit
GGAAAAAAAAAAAAAAAAAA648850.14709701651839305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA646710.14661186954243657No Hit
GGAAAAAAAAAAAA626810.14210045607443006No Hit
GGAAAAAAAAAAAAAAAAAAA624540.14158583755320522No Hit
TCCAGTGCGCCCCGGGCGTCGTCGC599400.13588649410668843No Hit
ACAAGCTTGGGCTGC583440.13226829516450833No Hit
TGAATACAAGCTTGGGCTGCAGGT579690.13141815443561267No Hit
AAAAAAAAA564050.12787250083563165No Hit
GGAAAAAAAAAAAAAAAAAAAA554970.12581402675073222No Hit
GGGAGAGCCTC543810.12328400794153861No Hit
GGAAAAAAAAAAA540970.1226401680295216No Hit
CGGGAGAGATCC539190.12223663456353911No Hit
GGAAAAAAAAAAAAAAAAAAAAA513050.11631058692265016No Hit
TATTCCCAGGCGGTCTCCCAT496350.11252462687663466No Hit
TGAAGGGGGGACGGAGAAGGC493430.11186265062906786No Hit
GGGAGAGATCCC484040.10973389824391305No Hit
GGGAGAAAGCCTAC473150.10726508956719993No Hit
TGGGAGAAAGC456510.10349273177284676No Hit
TCGTCGGGTCCCGGGG451340.10232067108794254No Hit
GGGGAGAGCCTC446850.10130276925521142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)