FastQCFastQC Report
Tue 11 Jul 2023
SRR8494666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41616303
Total Bases619.7 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCCGGCGGCGC17495164.203919795566656No Hit
AGTCCGCCCGGAGGAT7114981.709661715986641No Hit
TAACCCGGCGGCGC6955081.6712392737048265No Hit
TAGTCCGCCCGGAGGAT4748781.1410864631584405No Hit
GGGAGAGATCC3839850.9226792682665734No Hit
TGGGAGAGATCC3794620.9118109314034935No Hit
GGGGAGAGATCC3591150.8629190343986105No Hit
GGGAGAAAGCCC3407060.818683966233137No Hit
TGGGGTCGGCGGC3157660.7587555290531213No Hit
TCGCCGAGGGCGC2837290.6817736789353922No Hit
CCGCCGAGGGCGC2786020.6694539877797411No Hit
TAGCCGGGTCCC2592040.6228424471054048No Hit
TTGGCCCCGGAT2521190.6058178690211863No Hit
AGCCGGGTCCCC2130270.5118835279529755No Hit
TAGCCGGGTCCCCG1901960.45702281627467006No Hit
TGGCCGGGAAGGC1706090.40995712665779077No Hit
TGGGAGAAAGC1559780.37480023153426195No Hit
GGGGAGAAAGCCC1518160.36479934318048385No Hit
GAGGGCGCACCACCGGC1506740.36205522628956255No Hit
GGGGAGAAAGC1461460.3511748749041932No Hit
TCGGCGGCGC1439490.3458956938101878No Hit
AGTCCGCCCGGAGGATT1382340.33216309483329165No Hit
AACCCGGCGGC1339490.32186664923119196No Hit
TCCGCCGAGGGCGC1338930.32173208658154956No Hit
GGGGGTCGGCGGC1324620.31829353030229524No Hit
TGGGAGAAAGCCC1291190.3102606206995369No Hit
AACCCGGCGGCGCGC1267300.30452008194961483No Hit
TAAGCTTGGGCTGC1158560.27839089887441465No Hit
TGAGGGCGCACCACCGGC1140590.2740728795635691No Hit
TGGGAGAAAGCCCC1139020.2736956235636789No Hit
TGGGGTCGGCGGCGA1038490.24953922504841433No Hit
GGGAGAAAGCCCC1025060.24631212436145516No Hit
TGACCGGCTC1002380.2408623370509389No Hit
AGCCGGGTCCC997390.239663287726447No Hit
TGGGGTCGGC951020.22852101975516662No Hit
TAGCCGGGTCCCC948600.22793951687635491No Hit
CAACCCGGCGGCGC947190.2276007073477911No Hit
GGTTGGCCCCGGAT927880.22296069883958697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA925430.22237198724740156No Hit
ACGGGGTCGGCGGC909920.21864508243319933No Hit
AAACCCGGCGGCGC903240.21703994225532242No Hit
TCGCCCCTCCCAC885790.2128468739762876No Hit
ACCGGCCCGTCTCGC831930.19990483056604044No Hit
TCGACGAAGCCGAGC826420.19858083020973774No Hit
TGGGGTCGGCGGCG816880.19628845935690153No Hit
TGTCCGCCCGGAGGAT810900.1948515224910776No Hit
TGGCCCCGGAT807730.19408980177792343No Hit
TCGCCGAATCCCGGGGC777420.18680659836602975No Hit
CAGTCCGCCCGGAGGAT775930.18644856560180273No Hit
GGGAGAGCGACGCTCAGAC772000.18550422414984818No Hit
ACCACCGGCCCGT756370.18174848448215114No Hit
TGAGGAGGAAGGACGCGCGAG753990.18117659322117102No Hit
CGGTTGGCCCCGGAT736830.17705320917141534No Hit
CGGTGGCGGCCCGGCGTCCGGC719120.17279766537647517No Hit
GAGGAGGAAGGACGCGCGAG716640.17220174507091607No Hit
TGGGCCCGGCTCGGCGCGGC710820.17080325467641852No Hit
TGGACCCGTCCCCCCGAC700880.1684147676452663No Hit
TAACCCCCGGGACGGGGAC681540.1637675504236885No Hit
TCGCCCCTCC652590.15681114201806923No Hit
ACCGGCCCGTCTCG643280.1545740379677647No Hit
TCGGGACGGCCGGG642820.15446350436270131No Hit
GGGGTCGGCGGC637940.15329088698724633No Hit
AGGGAGAGATCC626390.1505155323383723No Hit
TGGCCCGAGGT614940.14776420673407725No Hit
GGCGGGAGAGGC605180.14541897198316725No Hit
TGGAGGATT602110.1446812803145921No Hit
TGAACGGGGAGGAGGC596460.14332363929587885No Hit
TGGCCGGGAAGGCC589610.1416776497422176No Hit
AACCCCCGGGACGGGGAC587300.1411225788124428No Hit
TGGGGCCGAGGAAGCC582520.1399739904815668No Hit
TAACCCGGCGGCGCGC576750.13858751460935875No Hit
CGCCCCTCCCAC558430.1341853936424867No Hit
ACCGGCCCGT545860.13116494273890691No Hit
TGGGAGAAAGCC542470.13035035812767895No Hit
GGGGAGAAAGCCCC538140.12930990049740843No Hit
TAACCCGGCGGC536760.1289782996822183No Hit
TAGTCCGCCCGGAGGATT533460.12818534121111141No Hit
ACCCCCCCCGT525430.12625580893141805No Hit
CCCCGGTCGCCGCGGT524780.1260996201416546No Hit
GGGGATCCCGAGGC521120.12522015711006332No Hit
GGGATCCCGAGGC519810.12490537662607848No Hit
TGATGCCGACTGGC502430.120729128678249No Hit
TCGCCGAGGGCGCACCA500730.12032063492040607No Hit
TGGAGGAGC500310.1202197129331743No Hit
CAGCCGGGTCCC483930.11628375543113476No Hit
GGACCCGTCCCCCCGAC479330.11517841938050094No Hit
AGCCGGGTCCCCG472320.11349398335551335No Hit
TCGCCCCTCCC469860.11290286885887003No Hit
TAGTCCGCCC456560.1097070059298636No Hit
ACGCTCCCCGGACCCCGTCCCGGC455130.10936339059238395No Hit
CCCCCGGCCGGCGACC450140.10816434126789205No Hit
GGGAGAAAGC434380.1043773638422423No Hit
TGGCGGTGCGCCGC428400.10294042697641835No Hit
CCGGGACGGCCGGG417690.1003669163020079No Hit
GGGGAGAAAGCC417330.1002804117415235No Hit
ACGGGGTCGGC416990.10019871298995492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)