FastQCFastQC Report
Tue 11 Jul 2023
SRR8494668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35618803
Total Bases873.2 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20367455.718173628687073No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTG6344821.7813119660422052No Hit
TCAAAAAGTAAGTTTAGATTATAGC4023051.1294736659174087No Hit
TGAATACAAGCTTGGGCTGC3348450.9400793170955238No Hit
TCTGGAACGGAAAAAACCTTTAATAGTG2803450.787070244892845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280160.6401562680250653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA2167650.6085690190094261No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2156510.605441457423485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA2128100.5974653331275619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA2082530.5846715286866884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2066450.5801570591802313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA2036260.5716811988319765No Hit
TCAAAAAGTAAGTTTAGATTATAG2017110.5663048250105429No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1996770.5605943579855842No Hit
AAAAAAAAAAAAAAAAAAAAAAA1926920.5409839291904335No Hit
TTCTGGAACGGAAAAAACCTTTAATAGT1891460.5310285132265674No Hit
AAAAAAAAAAAAAAAAAAAAAA1790150.502585670832341No Hit
AAAAAAAAAAAAAAAAAAAAA1714570.48136654114962824No Hit
TAGAGGGTATCAAGCACATTAAAATAGCT1620700.4550124831539117No Hit
AAAAAAAAAAAAAAAAAAAA1520980.42701603419968936No Hit
AGAGGGTATCAAGCACATTAAAATAGCT1455350.40859037289939254No Hit
AAAAAAAAAAAAAAAAAAA1429760.4014059652706465No Hit
AAAAAAAAAAAAAAAAAA1304880.3663458314418932No Hit
AAAAAAAAAAAAAAAAA1222810.34330463042230813No Hit
CAAAAAGTAAGTTTAGATTATAGC1077340.3024638419207967No Hit
AAAAAAAAAAAAAAAA1037920.2913966536157883No Hit
ACCTCTAGCATTACAAGT1030110.2892039914985352No Hit
TCCGCCGAGGGCGCACCACCGGC1021450.2867726913787642No Hit
TGAGGGTATCAAGCACATTAAAATAGCT976060.2740294220443062No Hit
ATCAAAAAGTAAGTTTAGATTATAGC946410.26570516701529806No Hit
AAAAAAAAAAAAAAA936640.2629622337392978No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG911050.2557778261105518No Hit
AAAAAAAAAAAAAA856540.24047411138437189No Hit
CAAAAAGTAAGTTTAGATTATAG852400.23931180393681395No Hit
TGAATACAAGCTTGGGCTGCAGGTC833730.2340701903991552No Hit
TCAAAAAGTAAGTTTAGATTATA807330.2266583747915392No Hit
AAAAAAAAAAAAA791200.2221298677555223No Hit
CCGCCGAGGGCGCACCACCGGC741230.2081007607133794No Hit
TCCGCCGAGGGCGCACCACCGG740270.20783124014582974No Hit
AAAAAAAAAAAA733790.20601197631486945No Hit
GGAAAAAAAAAAAAAAA721770.20263735420867454No Hit
GGAAAAAAAAAAAAAAAAA698850.19620255065842612No Hit
GGAAAAAAAAAAAAAAAA694280.19491952045665317No Hit
GGAAAAAAAAAAAAAAAAAA658630.1849107618804596No Hit
GGAAAAAAAAAAAAAAAAAAA653910.18358561909000704No Hit
GGAAAAAAAAAAAAAA652220.18311115059088315No Hit
AAAAAAAAAAA645820.18131434680721864No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA614620.17255492836185427No Hit
GGAAAAAAAAAAAAA608860.17093780495655622No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC607610.17058686671755927No Hit
GGAAAAAAAAAAAAAAAAAAAA592730.16640929792053932No Hit
CAAAAAGTAAGTTTAGATTATA586940.16478375199750536No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA564790.15856512640247905No Hit
CCGCCGAGGGCGCACCACCGG562700.15797835766687612No Hit
GGAAAAAAAAAAAAAAAAAAAAA542360.1522678906419174No Hit
GGAAAAAAAAAAAA534640.15010049607787213No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG527450.14808189932716156No Hit
TCTGGAACGGAAAAAACCTTTAATAGT517220.14520982077921035No Hit
TGAATACAAGCTTGGGCTGCAGG503160.14126246746697244No Hit
TGAATACAAGCTTGGGCTGCAG498660.13999908980658332No Hit
AAAAAAAAAA489940.1375509446513405No Hit
ATCAAAAAGTAAGTTTAGATTATAG486430.13656551007623696No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG481080.13506349441332993No Hit
GGAAAAAAAAAAA461200.12948217266032214No Hit
TCGACTGCATAATTTGTG458220.12864553589855335No Hit
GGAAAAAAAAAAAAAAAAAAAAAA453840.127415848309108No Hit
AAAAGGTTTGGTCCTGGCCTTATAATT438910.12322424198252817No Hit
ATTATATAAATCAAAACATTTATCCT420800.11813984877594005No Hit
GGAAAAAAAAAAAAAAAAAAAAAAA407340.11436094581842068No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG407310.11435252330068477No Hit
AACTATAAATAAATAAATAGAATTAAA402850.11310037566394356No Hit
CTGGAACGGAAAAAACCTTTAATAGTG400670.11248833937513286No Hit
GAATACAAGCTTGGGCTGC399810.11224689386670292No Hit
TGAATACAAGCTTGGGCTGCAGGT392390.11016372448001692No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG385710.1082883105308171No Hit
AATAATCCACCTATAACTTCTCTGT383210.10758643405282317No Hit
GAGAGGGTATCAAGCACATTAAAATAGCT373410.10483507825908693No Hit
TCGTCGCGGCGTGGGAAATGTGGC371280.10423707949983609No Hit
GGAAAAAAAAAA369880.10384402867215947No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG366700.10295124179215119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)