FastQCFastQC Report
Tue 11 Jul 2023
SRR8494670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27927253
Total Bases702 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17921686.417272762201137No Hit
TGAATACAAGCTTGGGCTGC4057071.45272791419908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2352800.8424745534406839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA2206080.78993805799661No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2141630.766860242215731No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA2114680.7572101702949445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA2100190.7520216900674048No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2064600.739277865961253No Hit
AAAAAAAAAAAAAAAAAAAAAAAA2005340.7180584499306109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1996630.7149396326233733No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG1930680.6913247070880906No Hit
AAAAAAAAAAAAAAAAAAAAAAA1795480.6429132145578371No Hit
AAAAAAAAAAAAAAAAAAAAAA1606510.575248127698059No Hit
AAAAAAAAAAAAAAAAAAAAA1501450.5376289604996238No Hit
AAAAAAAAAAAAAAAAAAAA1308780.4686390029123165No Hit
AAAAAAAAAAAAAAAAAAA1187960.4253766025609465No Hit
TCCTTAGGTCGCTGGTTCGTTTCCG1153210.4129335599172608No Hit
AAAAAAAAAAAAAAAAAA1057000.3784833402698074No Hit
AAAAAAAAAAAAAAAAA967140.34630688524932973No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG951490.34070304014505115No Hit
TGAATACAAGCTTGGGCTGCAGGTC927310.3320448309040635No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG908010.3251340187307359No Hit
AAAAAAAAAAAAAAAA823610.2949126432162877No Hit
TGAATACAAGCTTGGGCTGCAG802970.28752201299569274No Hit
AAAAAAAAAAAAAAA743930.2663813730623631No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC728140.2607273977143402No Hit
AGATCGGAAGAGCGGTTCAGAA697970.24992433018743376Illumina Paired End PCR Primer 2 (95% over 22bp)
AAAAAAAAAAAAAA689390.24685206239224458No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTG648590.23224267707246393No Hit
AAAAAAAAAAAAA644820.23089274122306264No Hit
TGAATACAAGCTTGGGCTGCAGG637090.2281248356220356No Hit
AAAAAAAAAAAA608510.21789110443479706No Hit
GAATACAAGCTTGGGCTGC599490.2146612844449828No Hit
TGAATACAAGCTTGGGCTGCAGGT596040.2134259320098543No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA581230.20812286836804178No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG577500.20678725544542456No Hit
AAAAAAAAAAA567640.20325665399314424No Hit
CCTGCGGTCCGGGTTCCTCCCTCGGGGGG514240.18413554673637253No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG491790.17609680407879713No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA486840.17432434188926493No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG470880.16860949410240955No Hit
AAAAAAAAAA470650.168527137273401No Hit
TGAATACAAGCTTGGGCTGCA449000.16077485315150758No Hit
AGATCGGAAGAGCGGTTCAAA443460.15879112779190993No Hit
GGAAAAAAAAAAAAAAAAAAA397150.1422087593076197No Hit
TGGGAGAAAGCCTACAGCACCCGGTATTCCCAG389600.13950530687712107No Hit
GGAAAAAAAAAAAAAAAAA388130.13897893931780544No Hit
GGAAAAAAAAAAAAAAAAAAAAA387100.1386101239531149No Hit
GGAAAAAAAAAAAAAAAAAA386930.1385492515142825No Hit
GGAAAAAAAAAAAAAAAAAAAA385860.13816611322280786No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG369230.13221135641231882No Hit
GGAAAAAAAAAAAAAAAA367300.13152027519498605No Hit
GGAAAAAAAAAAAAAAA363750.1302491154428973No Hit
GGAAAAAAAAAAAAAAAAAAAAAA362730.1298838808099028No Hit
AAAAAAAAA362710.12987671934651074No Hit
TCAAAAAGTAAGTTTAGATTATAGC342800.12274748253972563No Hit
TTGGGCCCCCCACGAGCGGCGCCGGGGAGCGG332180.11894474547854741No Hit
GGAAAAAAAAAAAAAA324810.11630574621857724No Hit
TGGGAGAGCGACGCTCAGACAGGCGTAG323490.11583308963470197No Hit
CGAATACAAGCTTGGGCTGC319760.1144974767120848No Hit
GGAAAAAAAAAAAAAAAAAAAAAAA311790.11164363355035314No Hit
GGAAAAAAAAAAAAA303690.10874324087657315No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGA291290.10430313357350256No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290880.10415632357396554No Hit
GGGGAGAGATCCTTCCGCAGGTTCACCTACG289910.10380899259945116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)