FastQCFastQC Report
Tue 11 Jul 2023
SRR8494674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40168596
Total Bases562 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGAGAGATCC7285301.8136805179847462No Hit
GGGAGAGATCC6023371.4995221640308265No Hit
GGGGAGAGATCC4701531.1704491737774456No Hit
GGGAGAAAGCCC4644621.1562813895710966No Hit
TGGGAGAAAGC3488130.8683723971831129No Hit
TCGCCCCTC2842230.7075751415359401No Hit
TAAGCTTGGGCTGC2567320.6391361052300658No Hit
TTGGGGGTGGGGGCC2536690.6315107453593847No Hit
TGGAGGAGC2345210.5838416657629756No Hit
GGGGAGAAAGC2182730.5433921563999897No Hit
TGGCGGGGTC2133410.5311139079892163No Hit
TGGGAGAAAGCCC2099000.5225475144812132No Hit
TGGGCGCGCGCCGCGGC2038470.5074785287491751No Hit
GGGAGAGC2004450.4990092260132767No Hit
TGGACGAGGCGCCGCCGC1992280.49597949602221597No Hit
TCGCCCCTCC1988110.4949413716127893No Hit
TGGGAGAGC1891690.4709375453401458No Hit
GGGGAGAAAGCCC1891050.47077821689361515No Hit
TGGGAGAAAGCCCC1655100.41203829977029816No Hit
TGTCCCCGCT1627810.4052444352299493No Hit
AGAAGGAC1539550.3832720466505725No Hit
ACCGGGGCCCGCGCGGCC1420060.3535249277818921No Hit
TGGGGGTGGGGGCC1378960.3432930541062476No Hit
TGAACGGGGAGGAGGC1319590.32851285118354645No Hit
GGGAGAAAGCCCC1301790.3240815287644109No Hit
TGGACCCGTCCCCCCGAC1239690.3086216904369772No Hit
TCGGCGGGGTC1159860.2887479562392472No Hit
TGGACGAGGCGCCGCCG1127210.28061971595920354No Hit
GGCGGGAGAGGC1108940.276071386712147No Hit
AGGGAGAGATCC1101830.27430134725146976No Hit
TGGGAGAAAGCC1090220.2714110296511235No Hit
ACGCGCGCGC1031680.2568374558075169No Hit
GGGAGAAAGCCT1030260.25648394581677686No Hit
TAACCCCCGGGACGGGGAC1025800.2553736257050159No Hit
GGGAGAAAGC995690.24787772019714108No Hit
TCGGGAGGGAGGAAGAC994120.2474868676017454No Hit
AACCCCCGGGACGGGGAC939680.23393399161872624No Hit
TCGCCCCTCCC900850.22426723602687035No Hit
TGGGCGCGCGCCGCGGCT898520.22368718090121945No Hit
GGAGGAGC859670.21401544629540947No Hit
TGGGAGGGAGGAAGAC851860.21207114134633934No Hit
TGGGGTCGCGCGCCCTC823320.2049660884338601No Hit
GAGGAGGAAGGAC764230.1902555917065162No Hit
GGACCCGTCCCCCCGAC756840.1884158460504818No Hit
TCGCCCCTCCCAC753580.18760426677596598No Hit
TGGGCCGCCCCTCCCAC749270.1865312892688607No Hit
CCGGAGCGGAC735420.18308332210565686No Hit
GGCGGGGTCC732880.18245098733348808No Hit
TGGACGAGGCGCCGCC728740.1814203314449925No Hit
GGGGAGGGCGGCGC719360.17908517390052667No Hit
GGGGAGAAAGCCCC718430.1788536497516617No Hit
GGGGAGAGC697840.17372775488593128No Hit
TGGCGGGGTCC691450.17213695992760117No Hit
ACCGGGGCCCGCGC661710.16473316617787687No Hit
TGGCGGGAGAGGC651580.16221129561013287No Hit
TCGCCCGTCCCCGCCCCTT648880.1615391287263314No Hit
ACCCCCCCCGT647940.1613051150704894No Hit
GGGGTCCCGGGG642700.16000061341451915No Hit
TGTCCCCGCTC642500.15995082327497828No Hit
TAGGCGGTCTCCCAT638250.15889278280973523No Hit
GGAAGGAC621030.15460585179526812No Hit
TGGACCCCGTCCCGGCCC619000.1541004818789285No Hit
GGGGCGCGCGCCGCGGC617950.15383908364633905No Hit
CCCCGGTCGCCGCGGT609830.15181760398098057No Hit
GGGGAGAGCCTC591580.14727425374787806No Hit
TAGCCCCGGGAGGAACCCGGGGCC583070.14515568331041492No Hit
AGGAAGAC580810.14459305473360332No Hit
GGGGAGAAAGCC571970.1423923305658978No Hit
GAAGAGGGAAAC556900.1386406435514948No Hit
GGGAGAGCGACGCTCAGAC556180.13846139904914775No Hit
TGATGCCGACTGGC544790.13562585060229637No Hit
TGGGGAGAGATCC539590.13433130697423432No Hit
TACGCGCGCGC535850.1334002313648204No Hit
ACCGGGGCCCGC534690.13311144855548349No Hit
CGGGAGAGATCC530570.132085771680942No Hit
GGGAGAGCCTC527690.13136879367155377No Hit
GGGTCCCGGGGG519490.12932739795037895No Hit
GGGAGAGATCCC518940.1291904750666416No Hit
GGGAGAGCGACGCTC518540.12909089478755992No Hit
TCGTCCCCGCT516170.12850088163400084No Hit
GGGTCCCGGGG503300.1252968861545472No Hit
GGCGGGGTC488770.12167963251690449No Hit
AAAAAAAAAAAA480540.11963076827479854No Hit
AAAAAAAAAAAAA477570.11889138470261695No Hit
CGGGAGGGAGGAAGAC472620.11765907874898092No Hit
TTGGCGGGGTC466130.11604338872088035No Hit
AAAAAAAAAAA463520.11539362739987227No Hit
GGGGAGGGAGGAAGAC463050.11527662057195127No Hit
AAAAAAAAAAAAAA462280.11508492853471901No Hit
GGGAGAAAGCC459970.1145098524230222No Hit
TCGGCGGGGTCC456480.11364101448803439No Hit
AAGGGAAAGA454120.11305349084145236No Hit
TAAGGGAAAGA453860.11298876366004926No Hit
GGGGGGTGGGGGCC451340.11236140790183456No Hit
GGGGGCGCGCCGACC449060.11179380031106888No Hit
AGGCGGTCTCCCAT448610.11168177249710196No Hit
GGGAGAGATCCTT446330.11111416490633629No Hit
TGGGGAGGGCGGCGC445270.11085027716676978No Hit
AAAAAAAAAAAAAAA444640.11069343822721611No Hit
TGGGAGAGAT442650.11019802633878464No Hit
AGGTCGACCC440490.10966029283174347No Hit
TGGGCGTGGGGGTGGGGGCC440120.10956818107359292No Hit
TGGGCGCGCGCCGC438480.10915990192935796No Hit
TGCCCCGGGCGTCGTCGC434550.10818152568738026No Hit
CGGTGGCGGCCCGGCGTCCGGC430610.10720065993842552No Hit
AAGCTTGGGCTGC417270.10387965763105089No Hit
TGGACCCCGTCCCGGC417130.10384480453337229No Hit
GGGGGGGT411930.10255026090531019No Hit
ACGCTCCCCGGACCCCGTCCCGGC410490.10219177190061608No Hit
GATGCCGACTGGC409040.10183079338894492No Hit
TGGGCCCGGCTCGGCGCGGC407880.10154201057960802No Hit
AGGAAGAGC406570.10121588516561544No Hit
AGTGCGCCCC405740.10100925608652093No Hit
AAAAAAAAAA402590.10022506138875256No Hit
TGGGAGAAAGCCT402450.10019020829107395No Hit
AACCCGGCGGCGC402190.10012548110967084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)