FastQCFastQC Report
Tue 11 Jul 2023
SRR8494676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32225169
Total Bases809.7 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGGAACGGAAAAAACCTTTAATAGTG18179755.641475456653152No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG6676062.0716912299203147No Hit
TCTGGAACGGAAAAAACCTTTAATAGTG4414881.370009882647939No Hit
TCCGCCGAGGGCGCACCACCGGC3126940.9703409158226601No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3001990.9315668755685967No Hit
TGAATACAAGCTTGGGCTGC2910460.9031636110271447No Hit
CCGCCGAGGGCGCACCACCGGC2844070.882561702003797No Hit
TCGCCGAGGGCGCACCACCGGC2842040.8819317596131148No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG2524040.7832511289545139No Hit
TCGCCGAGGGCGCACCACCGG2386430.7405484824610229No Hit
TCCGCCGAGGGCGCACCACCGG2274750.7058923414800401No Hit
CCGCCGAGGGCGCACCACCGG2122900.658770788758315No Hit
TTCTGGAACGGAAAAAACCTTTAATAGT1804930.5600994675931723No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG1753420.5441150673251706No Hit
TAGAGGGTATCAAGCACATTAAAATAGCT1690640.5246334006813123No Hit
TCAAAAAGTAAGTTTAGATTATAGC1658180.5145605287593682No Hit
CCTGCGGTCCGGGTTCCTCCCTCGGGGGG1622090.5033612081289628No Hit
TGAATACAAGCTTGGGCTGCAGGTC1539510.4777352758025878No Hit
AGAGGGTATCAAGCACATTAAAATAGCT1193450.3703471655959353No Hit
TGAGGGTATCAAGCACATTAAAATAGCT1164300.36130144111889684No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGA1072500.3328143911363196No Hit
TGAATACAAGCTTGGGCTGCAG1012410.3141674757392273No Hit
TTTCTGGAACGGAAAAAACCTTTAATAGTG986070.3059937404827885No Hit
CCCGCCGAGGGCGCACCACCGGC948810.2944313496075071No Hit
GAGAGGGTATCAAGCACATTAAAATAGCT914800.28387748719021455No Hit
TCAAAAAGTAAGTTTAGATTATAG801240.24863795128584118No Hit
TTGCGGTCCGGGTTCCTCCCTCGGGGGG746510.231654332053309No Hit
TGAATACAAGCTTGGGCTGCAGG741220.2300127580401518No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC722630.22424397526045556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA702990.2181493602097168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA674370.20926810345044275No Hit
ACCTCTAGCATTACAAGT672810.2087840097906081No Hit
CCCGCCGAGGGCGCACCACCGG663770.205978749095156No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG654980.20325106751185695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA648320.20118435996410136No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGA646040.20047683846126607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA639960.19859011445370542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA622030.1930261405300931No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA614130.19057464058605866No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGG609290.18907270897477682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA608760.1889082412570125No Hit
AAAAAAAAAAAAAAAAAAAAAAAA592510.18386559896706828No Hit
TCTGGAACGGAAAAAACCTTTAATAGT563630.17490365993115506No Hit
AAAAGGTTTGGTCCTGGCCTTATAATT558540.1733241492077202No Hit
AAAAAAAAAAAAAAAAAAAAAAA556360.17264765934974616No Hit
CTTTCGCCGAATCCCGGGGCCGA545020.16912867082248662No Hit
TGAATACAAGCTTGGGCTGCAGGT522560.16215896338666214No Hit
AAAAAAAAAAAAAAAAAAAAAA489500.15189990159555097No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGG482330.14967493265900328No Hit
TGAGGGCGCACCACCGGC481790.14950736177675283No Hit
GAGGGCGCACCACCGGC477640.14821954851501323No Hit
TCGTCGCGGCGTGGGAAATG475440.1475368523280669No Hit
CAAAAAGTAAGTTTAGATTATAGC464070.14400855430734902No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG453400.14069747780065947No Hit
TTCTGGAACGGAAAAAACCTTTAATAG451890.14022889996325544No Hit
AAAAAAAAAAAAAAAAAAAAA450870.13991237718567123No Hit
TCTTCTGGAACGGAAAAAACCTTTAATAGTG433690.1345811405985179No Hit
AGCTTTCGCCGAATCCCGGGGCCGA429810.1333771127779035No Hit
GCTGCGGTCCGGGTTCCTCCCTCGGGGGG413700.12837791479076496No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG396090.12291324213070845No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCGCGTC395890.12285117884098606No Hit
TGAATACAAGCTTGGGCTGCAGGTCGAC386350.11989075992122804No Hit
AAAAAAAAAAAAAAAAAAAA384570.11933839664269875No Hit
TGAATACAAGCTTGGGCTGCA383210.1189163662725865No Hit
CTGGAACGGAAAAAACCTTTAATAGTG376130.11671932581641387No Hit
TCGTCGCGGCGTGGGAAATGTGGC359720.11162703289469171No Hit
CGCCGAGGGCGCACCACCGGC357080.11080779747035616No Hit
CAAAAAGTAAGTTTAGATTATAG356910.11075504367409213No Hit
AAAAAAAAAAAAAAAAAAA345820.10731363425898557No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG340800.10575584568695358No Hit
TCAAAAAGTAAGTTTAGATTATA328380.10190171539519312No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)