FastQCFastQC Report
Tue 11 Jul 2023
SRR8494678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18450628
Total Bases411.7 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCCGAGGGCGCACCACCGGC2997691.6247089258967229No Hit
CCGCCGAGGGCGCACCACCGGC2559061.3869771803973285No Hit
TCCGCCGAGGGCGCACCACCGG1981901.0741639796759221No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG1804550.9780425902034336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1644650.891378873391193No Hit
CCGCCGAGGGCGCACCACCGG1568840.8502908410488792No Hit
CCCGCCGAGGGCGCACCACCGGC963170.5220255917576356No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG875480.47449875418874626No Hit
TGAATACAAGCTTGGGCTGC806270.4369878358611967No Hit
TCGCCGAGGGCGCACCACCGGC763830.41398590877231933No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTG689750.37383551389145125No Hit
TCAAAAAGTAAGTTTAGATTATAGC588800.31912192907471765No Hit
CCCGCCGAGGGCGCACCACCGG573680.31092708605907615No Hit
TCGCCGAGGGCGCACCACCGG566550.307062718949187No Hit
CCTGCGGTCCGGGTTCCTCCCTCGGGGGG559070.30300865639912095No Hit
TGAATACAAGCTTGGGCTGCAGGTC550610.2984234466165596No Hit
TGGCCCGAGGTGGGATC503890.2731018152878048No Hit
GAGGGCGCACCACCGGC472610.25614846280571046No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG468390.25386127778414913No Hit
TGGCCCGAGGTGGGATCCCGAGGCC421690.22855048619483304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA414530.22466985947578585No Hit
AAAAAAAAAAAAAAAAAAAAAAA408130.22120114285540848No Hit
AAAAAAAAAAAAAAAAAAAAAAAA406040.22006839008406653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA400660.2171525001750618No Hit
AAAAAAAAAAAAAAAAAAAAAA398490.2159763884459651No Hit
AAAAAAAAAAAAAAAAAAAAA397340.21535310342824104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA393640.21334775163208536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA388060.21032346432869387No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA384460.2083723112297316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA383850.20804169917685186No Hit
AAAAAAAAAAAAAAAAAAA372700.2019985444397882No Hit
AAAAAAAAAAAAAAAAAAAA372650.20197144509119147No Hit
AAAAAAAAAAAAAAAAA369760.20040510274230233No Hit
AAAAAAAAAAAAAAAAAA364830.1977331069706679No Hit
ACCTCTAGCATTACAAGT351680.19060597828973627No Hit
AAAAAAAAAAAAAAAA340990.1848121375597622No Hit
GGGAGAAAGCCTACAGC336450.182351516707182No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330640.17920257240024567No Hit
TGAATACAAGCTTGGGCTGCAG324570.17591271148060653No Hit
AAAAAAAAAAAAAAA324470.1758585127834131No Hit
TTTTGGTGACTCTAGATAACCTCGGGC318280.17250361342714188No Hit
TTCGGTTGGCCCCGGATAGCC308930.16743603523955933No Hit
TCGACTGCATAATTTGTG306790.16627618311962064No Hit
AAAAAAAAAAAAAA300190.1626990691048565No Hit
AAAAAAAAAAAAA283830.15383216224401686No Hit
TGAGGGCGCACCACCGGC283320.1535557488883305No Hit
TAGAGGGTATCAAGCACATTAAAATAGCT282670.15320345735657345No Hit
TCTGGAACGGAAAAAACCTTTAATAGTG276440.14982687852142484No Hit
TCGACGAAGCCGAGCGCACGGGGT275130.14911687558819137No Hit
AGATCGGAAGAGCGGTTCAGAA274140.14858030848597673Illumina Paired End PCR Primer 2 (95% over 22bp)
AGTCCGCCCGGAGGATTCAACCCG273680.14833099447888712No Hit
AAAAAAAAAAAA272680.14778900750695315No Hit
TGAATACAAGCTTGGGCTGCAGGT264750.14349105081951682No Hit
AAAAAAAAAAA257170.1393827895722574No Hit
CGAGGGCGCACCACCGGC253340.1373069794697503No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG252540.13687338989220313No Hit
GGGAGAGCGACGCTCAGAC250410.13571895764198377No Hit
TCTGCCGAGGGCGCACCACCGGC248990.13494933614183754No Hit
CGCCGAGGGCGCACCACCGGC244570.13255375372588943No Hit
GGAAAAAAAAAAAAAAA243690.13207680519058757No Hit
GGAAAAAAAAAAAAAA229930.12461906445677622No Hit
GAGGGCGCACCACCGG227810.12347005207627622No Hit
GGAAAAAAAAAAAAAAAA219400.11891194164231159No Hit
TCGACGAAGCCGAGCGCACGGGG219230.11881980385708281No Hit
GGAAAAAAAAAAAAA216080.11711254489549083No Hit
AAAAAAAAAA214070.11602315108190356No Hit
TGAATACAAGCTTGGGCTGCAGG207260.11233221980303325No Hit
TGAATACAAGCTTGGGCTGCA206050.11167641556699318No Hit
GGAAAAAAAAAAAAAAAAA203260.1101642719152974No Hit
GGGAGAGCGACGCTCAGACAGG202300.10964396442224081No Hit
TCAAAAAGTAAGTTTAGATTATAG201060.1089719005770427No Hit
AGATCGGAAGAGCGGTTCAAA201010.10894480122844599No Hit
TAGTCCGCCCGGAGGATTCAACCCG200570.10870632696079505No Hit
TAACGTCCGTCGTGAAGAGGGAAAC200090.10844617321426674No Hit
AGAGGGTATCAAGCACATTAAAATAGCT197700.10715082435134456No Hit
GGGAGAGCGACGCTCAGACA195530.10597471262224786No Hit
GGAAAAAAAAAAAA192590.10438127092476202No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGG191010.10352493150910635No Hit
TGAGGGTATCAAGCACATTAAAATAGCT190910.10347073281191296No Hit
CTTTCGCCGAATCCCGGGGCCGA186890.10129194518473843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)