FastQCFastQC Report
Tue 11 Jul 2023
SRR8494680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38999804
Total Bases945.2 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7020321.8000910978937228No Hit
TGAATACAAGCTTGGGCTGCAGGTC1477900.3789506224185127No Hit
TGAATACAAGCTTGGGCTGC1438130.368753135272167No Hit
TCTCGACTGCATAATTTGTGGT1268650.325296506618341No Hit
ACCTAATGGCCCAAAAACTATAGT1214350.3113733597225258No Hit
CTCGACTGCATAATTTGTGGT1189090.3048964040947488No Hit
ACCTAATGGCCCAAAAACTATAGTATA1161480.2978168813361216No Hit
ACCTAATGGCCCAAAAACTATAGTA1152410.29549122862258487No Hit
ACCTAATGGCCCAAAAACTATAGTATAA1075780.2758424119259676No Hit
ACCTAATGGCCCAAAAACTATAG1040500.26679621261686337No Hit
AAATCAAAAAGTAAGTTTAGATTATAGC1028720.26377568461626116No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG983150.25209101050866817No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG936800.24020633539594197No Hit
AAAAAAAAAAAAAAAAAAAAAAA906640.23247296319745606No Hit
AAAAAAAAAAAAAAAAAAAAA868970.22281394029569995No Hit
AAAAAAAAAAAAAAAAAAAAAA847700.21736006673264305No Hit
AAAAAAAAAAAAAAAAAAAAAAAA839410.21523441502423962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA826180.21184209028332554No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA822740.21096003456837886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA820650.21042413443923977No Hit
TGAATACAAGCTTGGGCTGCAGG814450.20883438285997538No Hit
AAAAAAAAAAAAAAAAAAAA814300.208795921128219No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA791450.20293691732399477No Hit
TCAAAAAGTAAGTTTAGATTATAGC784360.20111895946964245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA781180.20030357075640687No Hit
AAAAAAAAAAAAAAAAAAA777300.19930869396164144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA767020.19667278327860313No Hit
TAGTCGGCATTGGCAATTTTTGACAGTCTC725570.18604452473658586No Hit
ACCTAATGGCCCAAAAACTATAGTAT721270.1849419550929025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA684700.1755649848906933No Hit
AAAAAAAAAAAAAAAAAA679590.17425472189552543No Hit
GGGAGAGATCC660950.1694752106959307No Hit
ATCAAAAAGTAAGTTTAGATTATAGC638940.16383159258954225No Hit
ACCTAATGGCCCAAAAACTATAGTATAAG622370.15958285328818578No Hit
AAAAAAAAAAAAAAAAA617670.15837771902648534No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG609520.15628796493438787No Hit
TCACCTCTAGCATTACAAGTA600530.15398282514445458No Hit
TGAATACAAGCTTGGGCTGCAG577260.1480161284913124No Hit
TGAATACAAGCTTGGGCTGCA552950.14178276383132593No Hit
TAATCAAAAAGTAAGTTTAGATTATAGC550160.14106737562065697No Hit
TGAATACAAGCTTGGGCTGCAGGT540940.13870326117536386No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG508820.13046732234859437No Hit
AAAAAAAAAAAAAAAA498370.12778782170289882No Hit
ACCTAATGGCCCAAAAACTATA491190.1259467868094927No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC463850.118936495168027No Hit
TCGACTGCATAATTTGTGGT459500.11782110494709151No Hit
GGAAAAAAAAAAAAAAAAAA454830.1166236630317424No Hit
GGAAAAAAAAAAAAAAAAAAA454000.11641084144935701No Hit
GGAAAAAAAAAAAAAAAAA453520.11628776390773655No Hit
AAAAAAAAAAAAAAA425730.10916208707100168No Hit
TGGGAGAGATCC425720.10915952295555127No Hit
GGGGAGAGATCC411070.10540309382067664No Hit
GGAAAAAAAAAAAAAAAA403800.10353898188821668No Hit
GGAAAAAAAAAAAAAAAAAAAA400120.10259538740245977No Hit
TTGCGGTCCGGGTTCCTCCCTCGGGGGG395430.1013928172562098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)