FastQCFastQC Report
Tue 11 Jul 2023
SRR8494682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35639133
Total Bases708.4 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGAGCAGGATTACCATGGCAAC3112130.8732339251911655No Hit
AACGCTTGGTGAATTCTGCTTC2934470.8233842276690625No Hit
ACCGGGTCAGTGAAAAAAC2934020.8232579619711848No Hit
AAGACGGGTCGGGTGG2596380.7285194059013725No Hit
AGGACCGACTGACCCATGTTCAACTGCTGT1564550.4389977724766761No Hit
AAACGCTTGGTGAATTCTGCTTC1466550.4114999093833175No Hit
AACGCTTGGTGAATTCTGCTTCAC1371490.3848269821827596No Hit
AGATGACGAGGCATTTGGCT1214460.3407658654322483No Hit
TGAGCGGCGCCGGGGAGCGG1126490.31608232444936296No Hit
TAACGCTTGGTGAATTCTGCTTC886460.24873220120141531No Hit
AAGACGGGTCGGGTGGGT864880.24267705951208185No Hit
AAAAGAAAAGAGAACTCTCCCC752140.2110432933371303No Hit
AAAAACTGCGTGGCGGGGGTGCGTC738650.20725812830519755No Hit
TCGCCCCGGGCCCCTCGCGGGGAC723230.20293142372458947No Hit
AAACGCTTGGTGAATT706060.19811368587445716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA703180.1973055854080401No Hit
AGTTGATTCGGCAGGTGAGTTGTT695090.19503560875063936No Hit
TACTGAGCAGGATTACCATGGCAAC692620.19434255036451084No Hit
TAAGGGCCAGCGAGAGCTC662920.1860090143045848No Hit
ACGGCGGCTTTCGTGCGAGCCCCC659880.1851560193678112No Hit
TATCCTGAGGGAAACTTC651070.18268401759380623No Hit
ATGGGCCCGACGCTCCA633940.17787750336126304No Hit
AAAAACTGCGTGGCGGGGGTGCGT616330.1729363057176503No Hit
AAAAGAAAAGAGAACTCTCCCCGGGGCT599560.16823080404340926No Hit
AGTGGGTGAACAATCC579390.16257129487409247No Hit
CCGCCCCGGGCCCCTCGCGGGGAC564540.15840452684412948No Hit
AAAAGAAAAGAGAACTCTCCC545860.15316309743000764No Hit
TGATCGGCCGAGGGC545480.15305647306291095No Hit
ATGTCTCTTC527120.1479048325895021No Hit
AAGCCCGTTCCCTTGGCTGTGGTT517860.14530656511761944No Hit
AGTCCGCCCCGCCCCCCGA504810.1416448598791671No Hit
CCGGCCGCGCCCCGTT494930.13887262633465297No Hit
GGGAGAGATCC485480.13622104667922197No Hit
ACGGGAAGGGCCCGGCTCGCGTCC484360.13590678538672646No Hit
AAGCCAGTTATCCCTGTGGTAAC478820.1343523143506325No Hit
ACGCTCCCCGGACCCCGTCCCGGCCC477270.13391739916905387No Hit
TATGGGCCCGACGCTCCA474320.13308965737185582No Hit
ACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC466990.1310329294486485No Hit
ATGGGTACGGCCCGGCGCGAGATTT451580.1267090307724377No Hit
TAGTCGGATTCCCCTGGTCCGC449350.12608331409184392No Hit
TAACGCTTGGTGAATT432050.12122909948454694No Hit
ACCGTGCCAGACTAGAGTCAAGCT429570.12053323519402112No Hit
TGGACCCCGTCCCGGCCC423300.11877393313692564No Hit
AGGTAGGGACAGTGGGA417800.11723068571842081No Hit
TAAGCGAGCTTTTGCCC416450.11685188862478782No Hit
ACTGAGCAGGATTACCATGGC411630.11549944270529815No Hit
AGATGACGAGGCATTT409120.11479516070158047No Hit
ATGGGCCCGACGCTCCAGCGCCA394240.11061997495842563No Hit
TGAGCAGGATTACCATGGCAAC393710.11047126202536971No Hit
ACGAAACCCCGACCCAGAAGC392310.11007843540975029No Hit
AAAAAGGTCAGAAGGATC392010.10999425827783185No Hit
GCGGCCGGGCGCTTGGC387220.108650230071534No Hit
TACCGGGTCAGTGAAAAAAC386240.10837525144060042No Hit
ACCTTCCCCGCCGGGCCTTCCCAGC384700.10794314216341908No Hit
AGATCGCAGCGAGGGAGCTGCTCT374330.10503341930343817No Hit
GGGAGAAAGCCC372980.10465462220980515No Hit
TAAGCTTGGGCTGCAGGTC369990.10381565679501799No Hit
ATGGAAGCCTTCTCC357680.10036158848196447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)