FastQCFastQC Report
Tue 11 Jul 2023
SRR8494684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35515818
Total Bases824.9 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8061542.269844946271546No Hit
TGAATACAAGCTTGGGCTGC1514410.4264043700190152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1364130.3840908296128784No Hit
TGAATACAAGCTTGGGCTGCAGGTC1340180.37734735547974707No Hit
TCAAAAAGTAAGTTTAGATTATAGC1326280.3734336064003932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1319010.37138663116248655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1305000.36744190996811615No Hit
CCGCCGAGGGCGCACCACCGGC1299400.36586514774909595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1294860.3645868440929616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1291890.36375059698751694No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1289700.36313397033400724No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1272640.35833047685963476No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG1266990.35673963640651607No Hit
AAAAAAAAAAAAAAAAAAAAAAA1209670.3406003488361158No Hit
CCGCCGAGGGCGCACCACCGG1198320.3374045896957801No Hit
TCCGCCGAGGGCGCACCACCGGC1190610.3352337259978075No Hit
AAAAAAAAAAAAAAAAAAAAAA1130350.3182666382624216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1127480.31745854762517367No Hit
AAAAAAAAAAAAAAAAAAAAA1102280.31036311763958246No Hit
GAGGGCGCACCACCGGC1101620.31017728494948366No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG1095220.30837527098488904No Hit
TCCGCCGAGGGCGCACCACCGG1069200.3010489579600842No Hit
TCGCCGAGGGCGCACCACCGG1052550.29636090600531856No Hit
AAAAAAAAAAAAAAAAAAAA1006950.28352155650758204No Hit
AAAAAAAAAAAAAAAAAAA957410.269572842162892No Hit
TCGCCGAGGGCGCACCACCGGC956190.2692293332508912No Hit
GGGAGAGCGACGCTCAGAC908790.2558831673256125No Hit
AAAAAAAAAAAAAAAAAA892860.25139784194186376No Hit
AAAAAAAAAAAAAAAAA862960.2429790579510234No Hit
AAAAAAAAAAAAAAAA746470.21017958814858215No Hit
TCAAAAAGTAAGTTTAGATTATAG739990.2083550490094301No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG703160.19798502177255217No Hit
AAAAAAAAAAAAAAA688640.19389670259037817No Hit
GGGAGAAAGCCTACAGC675930.1903180154825661No Hit
GGGAGAAAGCCC674090.18979993646774518No Hit
AAAAAAAAAAAAAA628190.17687611756541832No Hit
TGAGGGCGCACCACCGGC620540.17472214774836384No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG619160.17433358848724814No Hit
ATCAGTAGGGTAAAACTAACCTGTCTCACGACG591880.16665250396316367No Hit
ACCTCTAGCATTACAAGT590200.1661794752974576No Hit
AAAAAAAAAAAAA583780.16437183003922365No Hit
AAGATCTGCACCTGCGGCGGCTCCACCCGGGCC542260.15268126444391622No Hit
AAAAAAAAAAAA536850.15115799951446987No Hit
CAAAAAGTAAGTTTAGATTATAGC528600.14883509088823463No Hit
CAAAAAGTAAGTTTAGATTATAG500500.1409231233249365No Hit
AAAAAAAAAAA485630.13673625650407378No Hit
GAGGGCGCACCACCGG474680.1336531232365252No Hit
GGGAGAGATCC470570.13249589239363713No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAC452610.12743899070549353No Hit
TGAATACAAGCTTGGGCTGCAGG439230.12367165526076297No Hit
AGTAGGGTAAAACTAACCTGTCTCACGACGGTC438770.12354213550705773No Hit
CTTTCGCCGAATCCCGGGGCCGA428410.12062512540187023No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTT418610.11786579151858476No Hit
TGGGAGAGATCC417740.11762083024527269No Hit
GGGGAGAGATCC405320.11412379689523129No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGG398900.11231615163699736No Hit
AAAAAAAAAA396930.11176146921352058No Hit
TGAATACAAGCTTGGGCTGCA386410.10879940875921822No Hit
TCGTCGCGGCGTGGGAAATG386190.10873746452918528No Hit
TGAATACAAGCTTGGGCTGCAG384930.10838269302990572No Hit
TGAATACAAGCTTGGGCTGCAGGT370510.10432253031592853No Hit
ATGGCCTCAGTTCCGAAAAC369610.10406912210215741No Hit
AAAAGGGCAGGGACTTAATCAACGCAAGCTT369180.10394804928891122No Hit
CCTGCGGTCCGGGTTCCTCCCTCGGGGGG367300.10341870768681156No Hit
TCTGGAACGGAAAAAACCTTTAATAGTG361910.10190107405100453No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG361770.10186165499552903No Hit
GGGAGAAAGCCTACAGCACCCGGT361140.10168426924588925No Hit
AAAAGGTTTGGTCCTGGCCTTATAATT355900.10020887031237743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)