FastQCFastQC Report
Tue 11 Jul 2023
SRR8494686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25838783
Total Bases605.1 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3403131.3170628043898198No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTG1829780.7081525472774782No Hit
TGAATACAAGCTTGGGCTGCAGGTC1187580.45961142984172276No Hit
TCAAAAAGTAAGTTTAGATTATAGC941450.3643553955308189No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG928480.3593358092755375No Hit
ACCTCTAGCATTACAAGT876970.33940065985305884No Hit
TGAATACAAGCTTGGGCTGC798940.3092018691437596No Hit
TAGAGGGTATCAAGCACATTAAAATAGCT743800.28786185479401255No Hit
TCCGCCGAGGGCGCACCACCGGC703110.27211420909413575No Hit
TCGACGAAGCCGAGCGCACGGGGT648540.25099479336933167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA614900.23797560434638118No Hit
AAAAAAAAAAAAAAAAAAAAAAAA606630.23477498920905063No Hit
AAAAAAAAAAAAAAAAAAAAAAA599170.23188785632821796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA580780.22477064806032077No Hit
AGAGGGTATCAAGCACATTAAAATAGCT578430.22386116250134536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA577600.22353993994221788No Hit
CCGCCGAGGGCGCACCACCGGC562500.21769601145688633No Hit
TCGACTGCATAATTTGTG552310.21375232726711627No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG547400.2118520829715548No Hit
AAAAAAAAAAAAAAAAAAAAA543620.21038916577456454No Hit
AAAAAAAAAAAAAAAAAAAAAA543080.21018017760356592No Hit
TCAAAAAGTAAGTTTAGATTATAG535890.20739753880823258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA531880.20584560813100214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA517560.20030355144822418No Hit
AAAAAAAAAAAAAAAAAAAA495070.1915995811412635No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490140.1896915965430725No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG488810.18917686641820552No Hit
AAAAAAAAAAAAAAAAAAA477930.184966141787715No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG477630.18485003724827134No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGG472660.18292657204482116No Hit
TCCGCCGAGGGCGCACCACCGG464190.1796485538811948No Hit
GAGAGGGTATCAAGCACATTAAAATAGCT461750.17870423696038626No Hit
AAAAAAAAAAAAAAAAAA452190.17500437230344787No Hit
AAAAAAAAAAAAAAAAA440310.17040663254147845No Hit
GGGAGAGCGACGCTCAGAC419230.16224835356990303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA401210.1552743409006531No Hit
AAAAAAAAAAAAAAAA389810.15086236840179354No Hit
ATCAAAAAGTAAGTTTAGATTATAGC380990.14744889494214955No Hit
TCTGGAACGGAAAAAACCTTTAATAGTG375100.14516937581773878No Hit
CCGCCGAGGGCGCACCACCGG374510.1449410368901662No Hit
GGGAGAAAGCCC371730.14386513482465485No Hit
TGGGAGAAAGCCTACA368000.14242156838423853No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGA364360.1410128333056553No Hit
AAAAAAAAAAAAAAA355670.13764967181310359No Hit
TCGACTGCATAATTTGTGG341750.13226242118291717No Hit
TGAATACAAGCTTGGGCTGCAGG334700.12953396450599086No Hit
TCGACGAAGCCGAGCGCACGGGG326890.12651137632914058No Hit
AAAAAAAAAAAAAA323620.12524583684920454No Hit
AAGATCTGCACCTGCGGCGGCTCCACCCGGGCC317810.12299727893531208No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG306090.11846146159437927No Hit
AGTCCGCCCGGAGGATTCAACCCG304240.11774548360114329No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAC299600.11594973339108115No Hit
GGGAGAAAGCCTACAGCACCCGGT295560.11438619225990637No Hit
AAAAAAAAAAAAA294120.11382889047057672No Hit
ATCAGTAGGGTAAAACTAACCTGTCTCACGACG286010.11069019775428277No Hit
ATGTACACGGAGTCGAC276900.10716448990650991No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGG274740.10632853722251548No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTT271280.10498946486759844No Hit
AAAAGGTTTGGTCCTGGCCTTATAATTAA270070.10452117655850895No Hit
TGAATACAAGCTTGGGCTGCA269080.10413803157834484No Hit
TAACGTCCGTCGTGAAGAGGGAAAC268990.10410320021651173No Hit
GGGAGAAAGCCTACAGC266920.1033020788943504No Hit
GGGAGAGATCC264060.10219521561832072No Hit
TGAGGGTATCAAGCACATTAAAATAGCT262210.10147923762508473No Hit
AAAAAAAAAAAA258860.10018273693463041No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)