FastQCFastQC Report
Tue 11 Jul 2023
SRR8494688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42051348
Total Bases713.3 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6324741.504051665597022No Hit
TGGGAGAGATCC3973320.944873396210747No Hit
GGGGAGAGATCC3765140.8953672543386718No Hit
GGGAGAGATCC3661490.8707188173848791No Hit
GGGAGAAAGCCC3625870.862248220913156No Hit
GGGAGAGCGACGCT2094540.4980910481157465No Hit
CCCCGGTCGCCGCGGT1811630.4308137755774203No Hit
GGGGAGAAAGCCC1799950.4280362189578322No Hit
TAACCCCCGGGACGGGGAC1794060.42663555042278306No Hit
ACCGGGGCCCGCGCGGCC1683190.400270164942156No Hit
AACCCCCGGGACGGGGAC1621370.38556909043676796No Hit
TGGGAGAAAGCCCC1604760.3816191576070284No Hit
TGGACCCGTCCCCCCGAC1587600.37753843229948303No Hit
GGGAGAAAGCCCC1576820.37497489973448656No Hit
TGGACCCCGTCCCGGCCC1565060.37217831875449037No Hit
TGGGAGAAAGCCC1446240.34392238745830456No Hit
CGGTGGCGGCCCGGCGTCCGGC1419790.3376324582983642No Hit
GGAAAAAAAAAAAAAAA1419080.337463617099742No Hit
GGACCCGTCCCCCCGAC1377080.3274758278854699No Hit
GGAAAAAAAAAAAAAA1329930.3162633454699241No Hit
GGAAAAAAAAAAAAAAAA1322380.3144679214564061No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1320270.3139661539506415No Hit
GGCTCCCAAACCACGC1317790.31337639877798923No Hit
GGAAAAAAAAAAAAAAAAA1308870.3112551825924819No Hit
AAAAAAAAAAAAAAAAAAAAAAA1286570.3059521421287137No Hit
GGAAAAAAAAAAAAA1280950.30461568081004203No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1276700.30360501166335974No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1276460.30354793858213536No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1249610.2971628876201543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1215200.28898003459960425No Hit
GGAAAAAAAAAAAAAAAAAA1192230.2835176651174179No Hit
AAAAAAAAAAAAAAAAAAAAA1192040.28347248226144856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1175800.2796105370985967No Hit
AAAAAAAAAAAAAAAAAAAAAA1167330.27759633294038516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160040.27586273809819367No Hit
GGAAAAAAAAAAAAAAAAAAA1157240.27519688548390886No Hit
GGAAAAAAAAAAAA1117630.2657774490368299No Hit
AAAAAAAAAAAAAAAAAAAA1107910.26346598924724124No Hit
TGGGAGAAAGC1092850.2598846534004094No Hit
AAAAAAAAAAAAAAAAAAA1092670.2598418485894911No Hit
AAAAAAAAAAAAAAAAA1069170.2542534427196008No Hit
AAAAAAAAAAAAAAAAAA1032250.24547370039124547No Hit
GGAAAAAAAAAAAAAAAAAAAA1011330.2404988301445176No Hit
GGGGAGGGCGGCGC990520.23555011839335088No Hit
TGAGCCGCCCCGGCGGTGAACGG987980.23494609495039254No Hit
TAAGCTTGGGCTGC986750.23465359540911745No Hit
AAAAAAAAAAAAAAAA986440.2345798760125359No Hit
GGAAAAAAAAAAA973910.23160018556361142No Hit
GGGGAGAAAGC972620.23129341775203022No Hit
AAAAAAAAAAAAAAA961420.22863000729489102No Hit
GGAAAAAAAAAAAAAAAAAAAAA951550.22628287682953707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA948570.22557421940433395No Hit
GGGAGAAAGCCT929070.22093703155485053No Hit
ACGCTCCCCGGACCCCGTCCCGGC879960.2092584523093053No Hit
AAAAAAAAAAAAAA877020.20855930706430623No Hit
TGGGGTCGCGCGCCCT847660.2015773667945199No Hit
GGCGGGAGAGGC821180.19528030349942646No Hit
GGGGAGAAAGCCCC816910.19426487826264213No Hit
GGGAGAGATCCTT804990.1914302485618297No Hit
AAAAAAAAAAAAA792930.188562326230303No Hit
TGAGGAGGAAGGACGCGCGAG784720.18660995124341792No Hit
GGAAAAAAAAAA784420.18653860989188742No Hit
GGAAAAAAAAAAAAAAAAAAAAAA762460.18131642295985376No Hit
TGGGAGGGAGGAAGAC749620.17826301311434772No Hit
GGGGAGAGCGACGCT738210.17554966371113714No Hit
TGAACGGGGAGGAGGCGGGAAC736910.17524051785450492No Hit
TGGGCGCGCGCCGCGGC729170.17339991098501764No Hit
ACCCCCCCCGT726080.17266509506425337No Hit
AGGGAGAGATCC725600.17255094890180453No Hit
GAGGAGGAAGGACGCGCGAG724800.17236070529772315No Hit
CCCGGTCGCCGCGGTTCGCCGCCC712200.16936436853344153No Hit
TGGGAGAGCGACGCT699260.16628717823742534No Hit
TGGGGTCGCGCGCCCTC697700.16591620320946668No Hit
AAAAAAAAAAAA693250.16485797316176404No Hit
GGGGGCGCGCCGACC686530.16325992688748053No Hit
TCGGGAGGGAGGAAGAC683770.16260358645339978No Hit
GGAAAAAAAAAAAAAAAAAAAAAAA660710.15711981456575422No Hit
CCCCACCGGGGCCCGCGCGGCC633970.15076092209933437No Hit
GGAAAAAAAAA631870.15026153263862077No Hit
ACCGGGGCCCGCGCGGC618140.14699647678357422No Hit
AAAAAAAAAAA590930.14052581619975654No Hit
GAGGAGGAAGGAC587600.13973392719776784No Hit
TGGGGAGGGCGGCGC570900.13576259196256918No Hit
TGGACGAGGCGCCGCCG566840.13479710567185624No Hit
GGGGAGGGAGGAAGAC563900.13409796042685718No Hit
GCGGGGGAGACGGGTCGGGGC553650.1316604642495646No Hit
TGCGGGGGAGACGGGTCGGGGC552570.13140363538405475No Hit
CGGGAGGGAGGAAGAC552210.13131802576221813No Hit
TGGACCCCGTCCCGGC551390.13112302606803475No Hit
TGGCGGGAGAGGCCGGGAGG548500.13043577104829077No Hit
TGGCGGGAGAGGC538570.12807437231263072No Hit
CCCCGCACGCACGCGC533380.12684016693115285No Hit
TAGGCGGTCTCCCAT528190.12560596154967493No Hit
TCGCCCGTCCCCGCCCCTT523250.12443120729447248No Hit
TGGACGAGGCGCCGCCGC508040.12081420077187538No Hit
TCGGTGGCGGCCCGGCGTCCGGC505980.12032432349136585No Hit
TGAAGGGGGGACGGAGAAGGC497950.11841475331539908No Hit
TGAACGGGGAGGAGGC497760.11836957045942974No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAA494030.11748255965540035No Hit
ACGCTCCCCGGACCCCGTCCCGGCCC489140.11631969562545295No Hit
AGGCGGTCTCCCAT483120.11488811250474062No Hit
GGCGGGAGAGGCCGGGAGG476430.11329720036561015No Hit
TGGGAGAGATCCTT474270.11278354263459045No Hit
GGGAGAGATCCC472970.11247439677795823No Hit
AAACCCCCGGGACGGGGAC469700.1116967760462756No Hit
TGAGGAGGAAGGACGCGCGAGGGC460030.10939720648194204No Hit
GGGGCGCGCGCCGCGGC459030.1091594019768403No Hit
GGGAGGGAGGAAGAC452140.10752092893668949No Hit
AAAAAAAAAA450190.10705721015174116No Hit
GGGGAGAGATCCTT448020.10654117437567043No Hit
TAGCCCCGGGAGGAACCCGGGGCC444500.1057041025177124No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA436390.10377550798133749No Hit
GGAAAAAAAA429290.1020870959951153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)