FastQCFastQC Report
Tue 11 Jul 2023
SRR8494690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38815431
Total Bases767.3 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4189121.0792408823181687No Hit
GGGAGAAAGCCC3489400.8989723700349996No Hit
GGGAGAGATCC3441500.8866319170847285No Hit
GGGGAGAGATCC2548450.6565558939690763No Hit
TGGGAGAGATCC2484140.6399877409579711No Hit
TCGTCCCCGCTCGGCGGG1617660.41675693360199967No Hit
GGGAGAAAGCCCC1535130.3954947711388288No Hit
TGAATACAAGCTTGGGCTGCAGGTC1228480.31649268560228017No Hit
TCGTCCCCGCTCGGCGGGGTC1152330.29687419933582604No Hit
GGGGAGAAAGCCC1144640.29489302849683674No Hit
TGGGAGAAAGCC1129180.2909100764590247No Hit
CCGTCCCCGCTCGGCGGG1051250.27083300968627655No Hit
AAAAAAAAAAAAAAAAAAAAAAA1041480.2683159694916179No Hit
TGAATACAAGCTTGGGCTGC1018300.26234411772987914No Hit
AAAAAAAAAAAAAAAAAAAAA1007460.2595514139724482No Hit
AAAAAAAAAAAAAAAAAAAAAAAA965230.24867172027537193No Hit
AAAAAAAAAAAAAAAAAAAAAA955090.2460593571664836No Hit
AAAAAAAAAAAAAAAAAAAA954120.24580945655350317No Hit
AAAAAAAAAAAAAAAAAAA952680.2454384700765013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA949150.2445290379488508No Hit
TGGGAGAAAGCCC902910.23261624996512342No Hit
AAAAAAAAAAAAAAAAA899350.2316990889525354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA895950.23082314865961429No Hit
AAAAAAAAAAAAAAAAAA895740.2307690464650515No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA873340.22499814571168872No Hit
TCACCTCTAGCATTACAAGTA860000.2215613682094629No Hit
AAAAAAAAAAAAAAAA818020.21074608188686605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA801500.20649004258126105No Hit
CCGTCCCCGCTCGGCGGGGTC785520.20237312320453174No Hit
TAACGTCCGTCGTGAAGAGGGAAAC780370.20104633129025412No Hit
AAAAAAAAAAAAAAA772710.19907288933620237No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGG768150.19789809882569642No Hit
GGGAGAAAGCC764410.19693456450348315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA757860.1952470912921204No Hit
GGGGAGAAAGCC756190.19481685003059737No Hit
TGAATACAAGCTTGGGCTGCAGGT738210.1901846716580321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA732550.1887264886998163No Hit
AAAAAAAAAAAAAA708310.1824815496702845No Hit
TGGGAGAAAGCCCC701820.1808095342287968No Hit
AGGGAGAGATCC688170.17729289158221637No Hit
AAAAAAAAAAAAA661040.17030340330370156No Hit
GGGAGAGCGACGCTCAGAC658150.1695588540547186No Hit
TGAATACAAGCTTGGGCTGCAGG656410.16911057872834132No Hit
TGAATACAAGCTTGGGCTGCA656030.16901267951913249No Hit
GGGAGAAAGCCTACAGC620040.15974059388906436No Hit
CCCCCGCCCCTTGCCTCT606550.1562651719621508No Hit
AAAAAAAAAAAA598200.1541139656545357No Hit
GGGAGAGCGACCCC595480.15341321342019879No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590820.152212659959901No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG581630.14984504487403474No Hit
GGGAGAGCGACGCTCAGACAGG571480.14723010547016727No Hit
GGGAGAGCGACCCCC566540.14595741575045243No Hit
GGGAGAGCGACGCTCAGACA546740.14085635169167643No Hit
GGAAAAAAAAAAAAAAA543020.13989796995942155No Hit
AACGTCCGTCGTGAAGAGGGAAAC534230.1376334066727225No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG527920.13600776454086005No Hit
GGAAAAAAAAAAAAAA524380.1350957561182304No Hit
AAAAAAAAAAA523690.13491799176466698No Hit
CCGCCGAGGGCGCACCACCGGC518240.133513911001014No Hit
GGAAAAAAAAAAAAA515600.13283376912651054No Hit
TGAATACAAGCTTGGGCTGCAG491090.12651927013254083No Hit
ACCGCCCGTCCCCGCCCCTT485720.1251357997287213No Hit
GGAAAAAAAAAAAAAAAA485020.1249554590801787No Hit
ACCTCTAGCATTACAAGT483020.12444020008434274No Hit
GGGAGAAAGCCCCC477020.12289442309683486No Hit
GGAAAAAAAAAAAAAAAAA463920.11951947667410932No Hit
ACCTCTAGCATTACAAGTA457850.11795566562174718No Hit
GGAAAAAAAAAAAA456330.11756406878491185No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG443570.11427671639147843No Hit
TGGGGGGCGGGGCGGACTGT441910.11384905142493458No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGG441530.11375115221572574No Hit
TCCCCGCCCCTTGCCTCT441270.11368416854626708No Hit
GGGAGAGCGACGCTCAGACAG422940.10896181984943051No Hit
TCCGCCGAGGGCGCACCACCGGC415900.10714810818408792No Hit
GGGGAGAAAGCCCC415230.10697549642048289No Hit
GGGGAGAGCCTC411330.10597074137860275No Hit
GGGAGAGCCTC411270.10595528360872768No Hit
GGAAAAAAAAAAAAAAAAAA410860.1058496555145813No Hit
AAAAAAAAAA406890.10482686640784693No Hit
GGAAAAAAAAAAA405070.10435798072163618No Hit
TTACCCTACGCCGGCA402410.10367268625717437No Hit
ACCCCGAGGAAAAGAAAT394940.10174819390772706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)