FastQCFastQC Report
Tue 11 Jul 2023
SRR8494692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42081424
Total Bases959 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17149324.075270836842403No Hit
TGAATACAAGCTTGGGCTGC3108760.7387487647756407No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG2436460.578987060894137No Hit
TCCGCCGAGGGCGCACCACCGG2191870.5208640277952571No Hit
ACCTCTAGCATTACAAGT1989850.47285709723131036No Hit
CCGCCGAGGGCGCACCACCGG1950920.4636059844362681No Hit
TCCGCCGAGGGCGCACCACCGGC1867990.4438989517084783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1784090.4239614134730802No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1703930.4049126284319656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1691640.4019920998871141No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1647570.39151954553629176No Hit
CCGCCGAGGGCGCACCACCGGC1630620.3874916400167447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1629680.38726826354545413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1609680.3825155726669326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1571950.37354962132460157No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1549830.3682931452129567No Hit
AAAAAAAAAAAAAAAAAAAAAAA1495730.3554371163865558No Hit
AAAAAAAAAAAAAAAAAAAAAA1391180.3305924248190841No Hit
AAAAAAAAAAAAAAAAAAAAA1366980.3248416688560729No Hit
TCGCCGAGGGCGCACCACCGG1262220.29994707403437676No Hit
TGAATACAAGCTTGGGCTGCAGGTC1247270.29639443760268186No Hit
AAAAAAAAAAAAAAAAAAAA1240760.2948474367217231No Hit
AAAAAAAAAAAAAAAAAAA1191400.2831177956335318No Hit
AAAAAAAAAAAAAAAAAA1141120.2711695307649285No Hit
AAAAAAAAAAAAAAAAA1103130.2621417944411767No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG997050.23693352202149814No Hit
CAAAAAGTAAGTTTAGATTATA976780.23211666981611648No Hit
AAAAAAAAAAAAAAAA967310.22986627068513651No Hit
TGAATACAAGCTTGGGCTGCAG937820.2228584279847564No Hit
GAGGGCGCACCACCGGC918750.21832673723208607No Hit
AAAAAAAAAAAAAAA895340.21276371255877655No Hit
TCGCCGAGGGCGCACCACCGGC840350.1996961889882814No Hit
AAAAAAAAAAAAAA820180.19490310023729235No Hit
TGAATACAAGCTTGGGCTGCAGGT813520.19332045417474467No Hit
TCGACTGCATAATTTGTG793520.18856776329622305No Hit
TGAATACAAGCTTGGGCTGCAGG774590.18406934137970235No Hit
AAAAAAAAAAAAA770110.18300473862291353No Hit
CCTGCGGTCCGGGTTCCTCCCTCGGGGGG765600.1819330068298069No Hit
TCAAAAAGTAAGTTTAGATTATAG754040.17918595150202143No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC737850.17533864823585818No Hit
AAAAAAAAAAAA724950.17227316261921174No Hit
AGAGCCACCTTACAAATA715570.1700441505971851No Hit
CAAAAAGTAAGTTTAGATTATAG696750.16557186848049626No Hit
CAAAAAGTAAGTTTAGATTATAGC685120.16280817873463596No Hit
TTAACCTAGACTTACAA673900.16014191915178536No Hit
AAAAAAAAAAA663200.1575992295317763No Hit
TAGAGCCACCTTACAAATA652310.1550113893484213No Hit
CCCGCCGAGGGCGCACCACCGG635260.15095972037448163No Hit
TGAATACAAGCTTGGGCTGCA631920.15016602099776852No Hit
TGGCCCGAGGTGGGATCCCGAGGCC630860.14991412838120688No Hit
TCGTCGCGGCGTGGGAAATG628860.1494388592933547No Hit
TGAGGGCGCACCACCGGC618920.1470767719267295No Hit
TAACGTCCGTCGTGAAGAGGGAAAC616690.1465468468937743No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG588610.13987406890033No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG587140.13952474612075866No Hit
CAAAAAGTAAGTTTAGATT583830.13873817578036332No Hit
GGAAAAAAAAAAAAAAA580020.13783278816800495No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG577120.13714364799061934No Hit
CCCGCCGAGGGCGCACCACCGGC576100.1369012607558147No Hit
TCCATCTAAGGCTAAATACCG565190.1343086678815812No Hit
GGAAAAAAAAAAAAAAAA551550.13106733270242946No Hit
TCCTCTAGCATTACAAGT542570.12893337449797326No Hit
AAAAAAAAAA537710.12777847061449252No Hit
GGAAAAAAAAAAAAAAAAA534090.1269182335654801No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA525760.12493873781457586No Hit
GGAAAAAAAAAAAAAA521330.12388601678498333No Hit
TGAAGGGGGGACGGAGAAGGC504660.11992464893773556No Hit
TAGAGCCACCTTACAAATAA502180.11933531526879888No Hit
GGGAGAGCGACGCTCAGACAGG499660.11873647621810515No Hit
GGAAAAAAAAAAAAAAAAAA496510.117987927404738No Hit
GAGGGCGCACCACCGG494200.11743899160826876No Hit
TGGCCCGAGGTGGGATC493940.11737720662684799No Hit
GGAAAAAAAAAAAAAAAAAAA480160.1141026026115466No Hit
GGAAAAAAAAAAAAA477710.11352039797892771No Hit
TCCATCTAAGGCTAAATACC465990.11073532112411404No Hit
ACCTCTAGCATTACAAGTA455900.10833758857589991No Hit
TTAACCTAGACTTACAAG453530.10777439470679509No Hit
TTAACCTTTAATAGTG439530.10444751109182997No Hit
TAACCTTTAATAGTGAATAATTAA435930.10359202673369608No Hit
TCAAAAAGTAAGTTTAGATT435340.1034518223527797No Hit
TCAAAAAGTAAGTTTAGATTATA433410.10299318768300238No Hit
GGAAAAAAAAAAAA428360.10179313323617566No Hit
TCAAAAAGTAAGTTTAGATTATAGC427600.10161253098279184No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG427380.10156025138312809No Hit
GGAAAAAAAAAAAAAAAAAAAA427290.10153886427417474No Hit
ATTGATCATCGACACTTCGAACG424090.10077843373361128No Hit
AGATCGGAAGAGCGGTTCAGAA423520.10064298204357341Illumina Paired End PCR Primer 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)