FastQCFastQC Report
Tue 11 Jul 2023
SRR8494694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17412979
Total Bases392.4 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4780092.745130514428347No Hit
CAAAAAGTAAGTTTAGATTATA2840771.631409536530194No Hit
TAACCTTTAATAGTGAATAATTAA1460140.8385354395706788No Hit
TGAATACAAGCTTGGGCTGC1143580.6567400098512725No Hit
CAAAAAGTAAGTTTAGATTATAG1118390.6422737889938304No Hit
CAAAAAGTAAGTTTAGATTATAGC1026810.5896808351976993No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG973520.5590772262460089No Hit
TTAACCTAGACTTACAA850360.48834837508274714No Hit
TTAACCTTTAATAGTG830450.47691437519105717No Hit
TAATCCACCTATAACTTCTCTG813890.467404227616653No Hit
ACCTTTAATAGTGAATAATTAA750340.43090846201560346No Hit
AGAGCCACCTTACAAATA737000.423247509802889No Hit
TAATCCACCTATAACTTCTCTGT704730.40471535628682487No Hit
TAATCCACCTATAACTTCTCT622670.3575895887774286No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG603910.34681601580062776No Hit
CAAAAAGTAAGTTTAGATT599580.3443293648950016No Hit
TAACCTAGACTTACAA589290.33841997971742804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570700.32774403506717603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA555670.31911254243171144No Hit
AAAAAAAAAAAAAAAAAAAAAAAA543590.31217518840400604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA537790.30884433961586927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA537130.30846531199515026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA520820.29909873548920035No Hit
CAAAAAGTAAGTTTAGATTATAGCC520260.298777136295863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA519310.29823156623573716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA513750.29503854567331644No Hit
AAAAAAAAAAAAAAAAAAAAAAA506210.2907084422487387No Hit
CCTACTAAAAGTATTGGAGAAAGAA479610.2754324805652152No Hit
AAAAAAAAAAAAAAAAAAAAAA468860.26925892462168594No Hit
TCCATCTAAGGCTAAATACC466440.26786915667904954No Hit
AAAAAAAAAAAAAAAAAAAAA465550.26735804367535276No Hit
TGAATACAAGCTTGGGCTGCAGGTC452390.2598004626319253No Hit
ACCTCTAGCATTACAAGT447730.2571242979159396No Hit
CAAAAAGTAAGTTTAGAT432980.24865360487714366No Hit
TCCTCTAGCATTACAAGT428810.24625883945532812No Hit
ACTAAAAGTATTGGAGAAAGAA427940.2457592121371076No Hit
AAAAAAAAAAAAAAAAAAAA419460.24088928149514222No Hit
TTATCTTTAATCAGTGAAATTGAC413090.23723109067092998No Hit
TAATCCACCTATAACTTCTCTGTT409840.2353646667810258No Hit
TGAACGGAAAAAACCTTTAATAGTG409690.23527852413995332No Hit
AAAAAAAAAAAAAAAAAAA399370.22935191043416525No Hit
ATGCCTAAAGGAAAGATCCAAAAA392520.225418063158521No Hit
TCCATCTAAGGCTAAATACCG382220.21950293513820926No Hit
TGAATACAAGCTTGGGCTGCAG377070.2165453711280534No Hit
CAACCTTTAATAGTGAATAATTAA375040.21537957405220556No Hit
TTAATCAGTGAAATTGAC374680.21517283171363152No Hit
AAAAAAAAAAAAAAAAAA372520.21393237768218754No Hit
AAAAAAAAAAAAAAAAA355290.2040374596443262No Hit
ACTAATCCTAGCCCTAGCC336470.1932294296110964No Hit
ATGCCTAAAGGAAAGATCC334820.19228186055929888No Hit
ACGGAAAAAACCTTTAATAGTG332390.19088634977392438No Hit
TTTAATCAGTGAAATTGAC331860.19058197910880154No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG329160.18903141156949652No Hit
AAAAAAAAAAAAAAAA313800.1802104051236724No Hit
TCTACTAAAAGTATTGGAGAAAGAA298860.1716305980728513No Hit
TAGAGCCACCTTACAAATA297290.1707289717629591No Hit
AAAAACCTTTAATAGTG295270.16956891752984943No Hit
CAAAAAGTAAGTTTAGATTAT292900.1682078638009039No Hit
AAAAAAAAAAAAAAA284420.16333793315893852No Hit
TGAATACAAGCTTGGGCTGCAGGT282540.16225827872416318No Hit
ATTGATCATCGACACTTCGAACG279820.1606962254993818No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC271440.1558837232847981No Hit
CGGAAAAAACCTTTAATAGTG269190.15459158366871056No Hit
CCTGCGGTCCGGGTTCCTCCCTCGGGGGG266920.1532879583671467No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG264350.15181204778343785No Hit
TCCTCTAGCATTACAAG263740.1514617343764097No Hit
TGAATACAAGCTTGGGCTGCAGG261630.1502499945586565No Hit
GAATACAAGCTTGGGCTGC261570.1502155375022275No Hit
AAAAAAAAAAAAAA259900.14925648276495365No Hit
AAAACCTTTAATAGTG245480.14097530353651722No Hit
TTAACGGAAAAAACCTTTAATAGTG244240.14026319103698454No Hit
TAACGGAAAAAACCTTTAATAGTG242850.13946493589637934No Hit
AAAAAAAAAAAAA240700.13823022470767352No Hit
AAAAAAAAAAAA227350.1305635296522209No Hit
ATGCCTAAAGGAAAGATCCAAAA221400.1271465382230117No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG217500.12490682955512668No Hit
AAATGCCTAAAGGAAAGATCC214770.12333903348760715No Hit
ATTGGAGAAAGAAATTCGTACA213100.1223799787503333No Hit
TGAATACAAGCTTGGGCTGCA211910.12169658046449144No Hit
ATAATCCACCTATAACTTCTCTGT208370.11966361413518042No Hit
AAAAAAAAAAA206170.11840018873278375No Hit
ATAATCCACCTATAACTTCTCTG196990.11312825909914669No Hit
AAACCTTTAATAGTGAATAATTAA189400.10876944146087812No Hit
CAAAAAGTAAGTTTAGATTA188710.1083731853119446No Hit
CCTTTAATAGTGAATAATTAA183120.1051629362213094No Hit
CGGAATGCCTAAAGGAAAGATCC180200.10348602614176472No Hit
AGATCGGAAGAGCGGTTCAGAA178650.10259588551734887Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGCCTAAAGGAAAGATCC177970.10220537221115353No Hit
TGAAGGGGGGACGGAGAAGGC175510.1007926328975645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)