FastQCFastQC Report
Tue 11 Jul 2023
SRR8494696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39117525
Total Bases795.5 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5741421.467736008349199No Hit
TAAGCTTGGGCTGCAGGTC1860470.47561035622780323No Hit
TGAGCGGCGCCGGGGAGCGG1529870.39109580680270545No Hit
TAACGTCCGTCGTGAAGAGGGAAAC1454410.3718052202944844No Hit
ACAAGCTTGGGCTGCAGGTC1351080.3453899499009715No Hit
GGGAGAGCGACGCTCAGAC1320760.33763894827190627No Hit
AAAAAAAAAAAAAAAAAAAAAAA1089490.27851710965865045No Hit
AAAAAAAAAAAAAAAAA1089030.27839951530675827No Hit
AAAAAAAAAAAAAAAAAAAAA1088390.27823590577369095No Hit
AAAAAAAAAAAAAAAA1062180.2715355841147925No Hit
AAAAAAAAAAAAAAA1054980.269694976867785No Hit
AAAAAAAAAAAAAAAAAAA1036190.26489150323288607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1031840.26377946968781896No Hit
AAAAAAAAAAAAAAAAAAAA1028560.26294097083084883No Hit
AAAAAAAAAAAAAAAAAAAAAA1025720.2622149535278625No Hit
AAAAAAAAAAAAAAAAAA1017430.2600956987948496No Hit
AAAAAAAAAAAAAAAAAAAAAAAA981950.25102559530542895No Hit
AAAAAAAAAAAAAA979410.250376269971068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA918610.23483336432967064No Hit
TAACCTAGACTTACAAGTCAAAGT900600.23022928981319754No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA899870.23004267268954262No Hit
AAAAAAAAAAAAA895450.2289127443517963No Hit
TGGGAGAGCGACGCTCAGAC889310.2273431153939315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA840800.21494202406721796No Hit
TGGGAGAAAGCCTACA831110.21246487348062026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA798260.20406710291614819No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA794470.20309822771251504No Hit
GGGGAGAGCGACGCTCAGAC793150.20276078305056364No Hit
AGAAGGACTTGGGCCCCCCAC789810.20190694579986845No Hit
TATTCCCAGGCGGTCTCCCAT775690.1982973104765703No Hit
TGGGAGAGATCC762780.19499699942672752No Hit
AAAAAAAAAAAA762690.19497399183613992No Hit
GGAAAAAAAAAAAAA717650.18345997094652589No Hit
GGAAAAAAAAAAAAAAA716270.18310718789084943No Hit
GGAAAAAAAAAAAAAA708410.18109785831286618No Hit
GGGAGAGATCC698270.1785056697733305No Hit
CCCCGGTCGCCGCGGTTC683440.17471453012428573No Hit
GGGGAGAGATCC668460.1708850444909283No Hit
AACGTCCGTCGTGAAGAGGGAAAC663960.16973466496154857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA662390.16933331032574275No Hit
GGAAAAAAAAAAAA658340.16829796874930097No Hit
GGAAAAAAAAAAAAAAAA656540.1678378169375491No Hit
GGAAAAAAAAAAAAAAAAA640730.16379615019099497No Hit
GGGAGAAAGCCC640180.16365554824851522No Hit
AAAAAAAAAAA604750.15459822675386545No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG596450.15247641562189837No Hit
TGAATACAAGCTTGGGCTGCAGGTC584750.14948542884551105No Hit
GGAAAAAAAAAAAAAAAAAA583940.1492783605302227No Hit
GGAAAAAAAAAAA580670.1484424180722068No Hit
GGGGAGAAAGCCTACA563480.14404796826997618No Hit
GGAAAAAAAAAAAAAAAAAAA563170.14396871990239668No Hit
TTAACCTAGACTTACA558260.1427135280158957No Hit
TGAGCGGCGCCGGGGAGCGGGTCTTCC550130.14063517566614964No Hit
CCCCCGGTGGCGGCCCG544200.13911923108632257No Hit
GGGAGAGCGACGCTCAGACAGGC533540.1363941098011697No Hit
GGGAGAGCGACGCTCAGACAGG512350.1309771004172682No Hit
TGAATACAAGCTTGGGCTGCAGGT505800.12930265910228217No Hit
TAAGCTTGGGCTGCAGG505210.12915183156398571No Hit
ACAAGCTTGGGCTGC501590.12822641514257357No Hit
TGAATACAAGCTTGGGCTGC496400.1268996440853556No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG487230.12455542624437513No Hit
GGAAAAAAAAAAAAAAAAAAAA482390.12331812915055336No Hit
GGAAAAAAAAAA457100.11685299619543926No Hit
GGAAAAAAAAAAAAAAAAAAAAA445230.11381855063683094No Hit
GGGAGAGCGACGCTCA437550.11185523624002286No Hit
AAAAGAGGGGGACCTTC431380.11027793808529553No Hit
TGGGAGAGCGACGCTCAGACAGG424720.10857537638181353No Hit
TGGGAGAAAGCC418530.10699296542917784No Hit
TAAGCTTGGGCTGCAGGTCGACCC417720.10678589711388949No Hit
AAAAAAAAAA416740.10653537001638012No Hit
ACGCTCCCCGGACCCC414180.10588093188411075No Hit
TGGGAGAGCGACGCTCAGACAGGC400020.10226107096499587No Hit
GGGAGAAAGCCTACAGCACCCGGT399370.10209490503297436No Hit
TGAAGGGGGGACGGAGAAGGC395620.10113625542515792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)