FastQCFastQC Report
Tue 11 Jul 2023
SRR8494698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39204346
Total Bases798.2 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12903583.291364686966083No Hit
TGAGCGGCGCCGGGGAGCGG2975120.7588750492101054No Hit
TAAGCTTGGGCTGCAGGTC2276010.580550431832226No Hit
AAAAAAAAAAAAAAAAAAAAAAA2013680.5136369319870813No Hit
AAAAAAAAAAAAAAAAAAAAA1957220.4992354674147606No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1932570.4929478991946454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1918210.4892850399800063No Hit
AAAAAAAAAAAAAAAAAAAAAA1841010.46959334559489907No Hit
AAAAAAAAAAAAAAAAAAAA1837430.468680181528854No Hit
AAAAAAAAAAAAAAAAAAA1805020.46041324091976943No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1784290.45512556184459757No Hit
GGGAGAGCGACGCTCAGAC1777750.45345737944461567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1762740.44962872228502426No Hit
AAAAAAAAAAAAAAAAAA1663150.424225926380713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1654630.42205269793303013No Hit
AAAAAAAAAAAAAAAAA1652140.42141756426698207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600730.40830422219006024No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1595100.40686815691301165No Hit
TCGGCGGGAGAGGCCGGGAGG1451590.37026252140515237No Hit
AAAAAAAAAAAAAAAA1446000.3688366590785624No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1377040.35124677248792774No Hit
AAAAAAAAAAAAAAA1270060.32395898148638924No Hit
GGAAAAAAAAAAAAAAAAA1055990.26935534136955125No Hit
GGGAGAGATCC1052730.2685238009071749No Hit
GGGAGAGCGACGCTC1048570.26746269405948003No Hit
GGAAAAAAAAAAAAAAA1046110.26683521260627585No Hit
GGAAAAAAAAAAAAAAAA1031700.26315959970356345No Hit
ACGCTCCCCGGACCCCGTCCCGGCCC1024030.261203183953126No Hit
AAAAAAAAAAAAAA1021770.2606267172522148No Hit
GGAAAAAAAAAAAAAAAAAA975350.248786193245004No Hit
TGGCGGGAGAGGCCGGGAGG942410.24038406354234299No Hit
GGAAAAAAAAAAAAAAAAAAA932340.23781547076438922No Hit
TGGGAGAGATCC931040.23748387487448458No Hit
GGAAAAAAAAAAAAAA916060.2336628699277371No Hit
GGGAGAAAGCCC911950.23261451676811545No Hit
TGAGCGGCGCCGGGGAGCGGGTCTTCC894220.22809205897733892No Hit
TAAGCTTGGGCTGC893550.22792115955715728No Hit
CCCCGGTCGCCGCGGTTC842850.2149889198508757No Hit
GGGGAGAGATCC841270.21458590330776084No Hit
AAAAAAAAAAAAA803170.2048675929959398No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801020.2043191844087898No Hit
GGAAAAAAAAAAAAAAAAAAAA793050.20228624652991276No Hit
TAAGCTTGGGCTGCAGGTCGACCCGTG778620.1986055321519711No Hit
GGAAAAAAAAAAAAA775510.19781225275381462No Hit
GGCGGGAGAGGCCGGGAGG771160.1967026818914413No Hit
GGAAAAAAAAAAAAAAAAAAAAA756290.192909735058455No Hit
TGGACCCCGTCCCGGCCC707750.18052845467693812No Hit
TGGGAGAGCGACGCTCAGAC697050.17779916542926133No Hit
AAAAAAAAAAAA621030.15840845808268297No Hit
GGAAAAAAAAAAAAAAAAAAAAAA609250.15540368917262387No Hit
CCCCCGGTGGCGGCCCG607820.15503893369372873No Hit
GAGAAAAAAAAAAAAAAAAAA602660.15372275308456876No Hit
GAGAAAAAAAAAAAAAAAAA582070.14847078433600192No Hit
GGAAAAAAAAAAAA580320.14802440525343796No Hit
GGGGAGAGCGACGCTCAGAC579290.1477616792791289No Hit
GAGAAAAAAAAAAAAAAAA574900.14664190546629702No Hit
GAGAAAAAAAAAAAAAAAAAAAA566910.14460386611219073No Hit
GAGAAAAAAAAAAAAAAAAAAA549240.14009671274710206No Hit
GAGAAAAAAAAAAAAAAA526660.13433714721321968No Hit
GGGAAAAAAAAAAAAAA516160.1316588727178359No Hit
GGAAAAAAAAAAAAAAAAAAAAAAA509650.129998342530698No Hit
ACAAGCTTGGGCTGC507100.12934790443896196No Hit
GAGAAAAAAAAAAAAAAAAAAAAA498700.12720528484265495No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA497070.12678951461146679No Hit
GGGAAAAAAAAAAAAAAA492000.12549629064083864No Hit
GAGAAAAAAAAAAAAAA491740.12542997146285773No Hit
GGGAAAAAAAAAAAAAAAA480730.12262160934912675No Hit
AAAAAAAAAAA477970.12191760576748302No Hit
GGGAAAAAAAAAAAAA471600.12029278590695021No Hit
TGGGAGAAAGCC470080.11990507378952323No Hit
TAACCTAGACTTACAAGTCAAAGT454880.11602795261525342No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA450920.11501786051985154No Hit
AGAAGGACTTGGGCCCCCC444010.11325530082812757No Hit
AGGAAGAC435380.1110540142666836No Hit
AGAAGGACTTGGGCCCCCCAC431600.11008983544834544No Hit
GGGAAAAAAAAAAAAAAAAA428970.10941899145569219No Hit
ACAAGCTTGGGCTGCAGGTC418680.1067942824502161No Hit
GGGAAAAAAAAAAAA412950.10533270979702097No Hit
TGGACCCGTCCCCCCGAC412780.10528934725757191No Hit
GGAAAAAAAAAAA411260.10490163514014492No Hit
GGGAAAAAAAAAAAAAAAAAA406310.10363902002089259No Hit
ACCCCCACCACCACGCCCGCGGT400850.10224631728329305No Hit
GAGAAAAAAAAAAAAA393530.10037917734936835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)