FastQCFastQC Report
Fri 14 Jul 2023
SRR8494700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48163416
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length8-42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13271502.7555146835930406No Hit
TGAATACAAGCTTGGGCTGC3989900.8284088487411275No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG3430140.7121878564427406No Hit
TGAATACAAGCTTGGGCTGCAGGTC1968740.4087625346175612No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1795190.3727289609192172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1781690.3699260035874532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1723580.3578608294727268No Hit
AAAAAAAAAAAAAAAAAAAAAAA1700690.3531082595968691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1697580.3524625412782183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1693320.35157805252019503No Hit
TGAATACAAGCTTGGGCTGCAG1691330.3511648758468461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1661190.34490701407059665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1636410.33976203016829204No Hit
AAAAAAAAAAAAAAAAAAAAA1598710.3319345122862548No Hit
AAAAAAAAAAAAAAAAAAAAAA1585150.3291190973663496No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG1513280.3141969830379141No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1476710.30660408306586895No Hit
AAAAAAAAAAAAAAAAAAAA1473640.30596666980597886No Hit
AAAAAAAAAAAAAAAAAAA1423380.295531363473056No Hit
AAAAAAAAAAAAAAAAAA1342700.27878005995255817No Hit
AAAAAAAAAAAAAAAAA1325150.275136215421265No Hit
TGAATACAAGCTTGGGCTGCAGGT1218090.25290772564803127No Hit
AAAAAAAAAAAAAAAA1175220.2440067789211629No Hit
AAAAAAAAAAAAAAA1105500.22953106150111943No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG1054400.2189213489342201No Hit
AAAAAAAAAAAAAA1037090.2153273347554916No Hit
TGAATACAAGCTTGGGCTGCAGG1034370.2147625907597584No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG981870.20386220113623169No Hit
AAAAAAAAAAAAA965690.20050280486749528No Hit
GGGAGAAAGCCTACAGC952360.1977351440354646No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC950580.1973655689206098No Hit
GGGAGAGCGACGCTCAGAC945800.1963731143986963No Hit
TGAATACAAGCTTGGGCTGCA918960.19080041997021144No Hit
GGGAGAGATCC914970.18997199035882337No Hit
AAAAAAAAAAAA895260.18587967265444794No Hit
CCTGCGGTCCGGGTTCCTCCCTCGGGGGG852920.1770887679561599No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG833080.1729694588108119No Hit
TCTTCCGTACGCCACATG832690.17288848448789432No Hit
GAATACAAGCTTGGGCTGC807390.16763553482169952No Hit
AAAAAAAAAAA800910.1662901153024528No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG680460.14128150710904724No Hit
AAAAAAAAAA667480.1385865155411734No Hit
GGGAGAAAGCCC640750.1330366600242807No Hit
TAACGTCCGTCGTGAAGAGGGAAAC628050.1303998038677323No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG626640.13010705054641475No Hit
TGAAGGGGGGACGGAGAAGGC567450.11781763984514719No Hit
GGGAGAGCGACGCTCAGACAGG559880.11624590747466915No Hit
GGAAAAAAAAAAAAAAA547250.11362358517095217No Hit
TGGGAGAAAGCCCC539940.1121058356824192No Hit
AAAAAAAAA536390.11136876171739978No Hit
TGGGAGAAAGCCTACAGCACCCGGTATTCCCAG525110.10902673514685919No Hit
GGAAAAAAAAAAAAAAAA509460.10577738090670313No Hit
GGAAAAAAAAAAAAAA508040.10548255132069537No Hit
GGGAGAGCGACGCTCAGACA499230.10365336212863306No Hit
GGAAAAAAAAAAAAAAAAA484750.10064693085722988No Hit
GGAAAAAAAAAAAAA484410.10057633785776324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)