FastQCFastQC Report
Tue 11 Jul 2023
SRR8494702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32151946
Total Bases721.7 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAATACAAGCTTGGGCTGC4480601.3935703922866753No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4426431.3767222674484463No Hit
TGAATACAAGCTTGGGCTGCAGGTC2201400.6846863950318902No Hit
ATGTACACGGAGTCGAC1361230.4233740626461614No Hit
AAAAAAAAAAAAAAAAAAAAA1250550.38895001876402757No Hit
AAAAAAAAAAAAAAAAAAAAAAA1250170.3888318299613964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1249870.38873852301195083No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1241350.3860886056476955No Hit
AAAAAAAAAAAAAAAAAAAAAA1220650.37965042613594835No Hit
AAAAAAAAAAAAAAAAAAAA1186070.3688952450965176No Hit
TGAATACAAGCTTGGGCTGCAGG1166160.3627027738849773No Hit
AAAAAAAAAAAAAAAAAAA1165790.3625876953139944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1145750.3563547910910276No Hit
AGATCGGAAGAGCGGTTCAGAA1133170.3524421196776083Illumina Paired End PCR Primer 2 (95% over 22bp)
TGAATACAAGCTTGGGCTGCAG1129120.3511824758600926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1126130.3502525165972846No Hit
AAAAAAAAAAAAAAAAAA1116410.34722937143524685No Hit
AAAAAAAAAAAAAAAAA1098200.34156563960389835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1068620.3323655743885611No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1062010.33030971126910946No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1061320.33009510528538455No Hit
TGAATACAAGCTTGGGCTGCAGGT1053500.32766290413650234No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG1036770.32245948658908546No Hit
AAAAAAAAAAAAAAAA972220.3023829413000383No Hit
TCTTCCGTACGCCACATG958720.29818412857498583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910210.28309639484963056No Hit
AAAAAAAAAAAAAAA896900.2789566765258936No Hit
GAATACAAGCTTGGGCTGC845640.263013629097287No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCC843690.26240713392589055No Hit
ATGTACACGGAGTCGAGCTCAACCC835750.25993760999723003No Hit
TGAATACAAGCTTGGGCTGCA825540.2567620634844311No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG825310.25669052815652277No Hit
AAAAAAAAAAAAAA806690.25089927682759855No Hit
GGAAAAAAAAAAAAAAA801560.24930372799207862No Hit
GAGAGAGAGACGGGGAGGGCGG796020.24758065965898302No Hit
GGAAAAAAAAAAAAAAAA752650.23409158500079594No Hit
TGAAGGGGGGACGGAGAAGGC743440.23122706165281565No Hit
GGAAAAAAAAAAAAAA741430.23060190509153008No Hit
GGAAAAAAAAAAAAAAAAA723770.2251092360008318No Hit
AAAAAAAAAAAAA709780.22075802192501817No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG700140.21775975861616587No Hit
GGAAAAAAAAAAAAA688230.21405547272317513No Hit
GGAAAAAAAAAAAAAAAAAA657440.2044790694784073No Hit
TAACGTCCGTCGTGAAGAGGGAAAC648680.20175450655459548No Hit
AGATCGGAAGAGCGGTTCAAA629850.19589794036105934No Hit
AAAAAAAAAAAA628820.19557758650129603No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG615520.19144097840920735No Hit
GGAAAAAAAAAAAA613060.1906758614237533No Hit
GGAAAAAAAAAAAAAAAAAAA611270.19011912995872787No Hit
GGGAGAAAGCCTACAGC600270.18669787514572214No Hit
AAAAAAAAAAA551910.1716567948950897No Hit
GGAAAAAAAAAAAAAAAAAAAA527640.16410826268493983No Hit
GGAAAAAAAAAAA522900.16263401288369916No Hit
GGAAAAAAAAAAAAAAAAAAAAA477190.1484171440198363No Hit
TGAATACAAGCTTGGGCTGCAGGTCGAC445610.1385950324748617No Hit
AAAAAAAAAA442890.13774904946655483No Hit
CGAATACAAGCTTGGGCTGC427910.1330899224575707No Hit
GGAAAAAAAAAA422950.1315472475600699No Hit
GGGAGAGCGACGCTCAGAC414040.1287760311615353No Hit
GGAAAAAAAAAAAAAAAAAAAAAA388700.12089470416502938No Hit
GGGAGAGCGACGCTCAGACAGG383370.1192369506965457No Hit
GGGAGAAAGCCTACAGCACCCGGT371080.11541447600092386No Hit
GGGAGAGCGACGCTCAGACAG353160.10984094088737273No Hit
AAAAAAAAA351650.10937129590849648No Hit
GGGAGAGCGACGCTCAGACA350080.10888298953973113No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG336240.1045784289386403No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG335330.10429539785865528No Hit
GGAAAAAAAAA328880.10228929844557463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)