FastQCFastQC Report
Fri 14 Jul 2023
SRR8494704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35767216
Total Bases885.5 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12745953.563584596575814No Hit
TGAATACAAGCTTGGGCTGC1310910.36651161219816497No Hit
TCTGCGGTCCGGGTTCCTCCCTCGGGGGG1142810.31951326600314656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1122340.31379014793882753No Hit
TGAATACAAGCTTGGGCTGCAGGTC1113220.31124032689600445No Hit
AAAAAAAAAAAAAAAAAAAAAAA1111210.31067835975827696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1081730.30243617507160747No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1078680.30158343886759315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1072640.29989474159800417No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1052960.2943924961898069No Hit
AAAAAAAAAAAAAAAAAAAAA1048690.29319866550418683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1042940.2915910480703894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1037680.2901204276005155No Hit
AAAAAAAAAAAAAAAAAAAAAA1015170.28382695482924925No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1001020.2798708180139041No Hit
AAAAAAAAAAAAAAAAAAAA986820.275900701916526No Hit
AAAAAAAAAAAAAAAAAAA968590.2708038556872864No Hit
TGCGGTCCGGGTTCCTCCCTCGGGGGG955090.26702944953837054No Hit
TCTTCCGTACGCCACATG944520.2640742293165898No Hit
AAAAAAAAAAAAAAAAA940340.26290556133862925No Hit
AAAAAAAAAAAAAAAAAA919940.2572020142691564No Hit
CTGCGGTCCGGGTTCCTCCCTCGGGGGG884290.2472347861796121No Hit
TAACGTCCGTCGTGAAGAGGGAAAC880000.24603536378117885No Hit
AAAAAAAAAAAAAAAA857430.23972511587147294No Hit
AAAAAAAAAAAAAAA801120.22398164844588406No Hit
GGGAGAGCGACGCTCAGACAGGCGTAG747340.20894553269116614No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGG708260.1980193258541565No Hit
AAAAAAAAAAAAAA699490.19556735978556455No Hit
ATCAGTAGGGTAAAACTAACCTGTCTCACGACG691050.19320765697839049No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACG639320.17874469178702643No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCG619400.17317534582507063No Hit
AAAAAAAAAAAAA601470.16816237528802913No Hit
TGAATACAAGCTTGGGCTGCAGGT548750.15342262031241122No Hit
TGGGAGAGATCCTTCCGCAGGTTCACCTACG536310.14994457494259547No Hit
TGAATACAAGCTTGGGCTGCAGG526520.14720743152052987No Hit
GGGAGAAAGCCTACAGCACCCGGTATTCCCAG494180.13816563190157152No Hit
AAAAAAAAAAAA493500.13797551366592245No Hit
TGAATACAAGCTTGGGCTGCAG484460.13544805947435215No Hit
ATTTAAAGTTTGAGAATAGGTTGAGATCGTT469420.1312430914388193No Hit
TGGGAGAAAGCCTACAGCACCCGGTATTCCCAG452830.12660476566026274No Hit
TGAATACAAGCTTGGGCTGCA427600.11955081994640007No Hit
TTGCGGTCCGGGTTCCTCCCTCGGGGGG396040.11072709712715689No Hit
AAAAAAAAAAA389370.10886226090395183No Hit
AACGTCCGTCGTGAAGAGGGAAAC379210.10602167079484186No Hit
GGAAAAAAAAAAAAAAA367150.10264986796847705No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA363180.10153991297505514No Hit
GGAAAAAAAAAAAAAA358860.10033210300740208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)