FastQCFastQC Report
Tue 11 Jul 2023
SRR8494706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28814895
Total Bases597.5 Mbp
Sequences flagged as poor quality0
Sequence length8-42
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5269561.8287625202174085No Hit
TGGACCCCGTCCCGGCCC1619570.562060004036107No Hit
CCCCGGTCGCCGCGGTTC1451330.5036735341218491No Hit
ACGCTCCCCGGACCCCGTCCCGGCCC1382640.4798351685820823No Hit
TGAGCGGCGCCGGGGAGCGG1350750.4687679757292192No Hit
GGGAGAGCGACGCTCAGAC1311780.4552437203050714No Hit
TAAGCTTGGGCTGCAGGTC1188910.4126025793257272No Hit
CCCGGTCGCCGCGGTTC928750.3223159411130945No Hit
AAAAAAAAAAAAAAAAA887430.3079761352592123No Hit
AGAAGGACTTGGGCCCCCC881510.3059216422617539No Hit
AAAAAAAAAAAAAAAA853800.29630508804560973No Hit
AAAAAAAAAAAAAAA852130.2957255266763943No Hit
AAAAAAAAAAAAAAAAAAAAA841480.29202952153738543No Hit
AAAAAAAAAAAAAAAAAAA838450.2909779820471322No Hit
AAAAAAAAAAAAAAAAAA832340.28885755092982296No Hit
AAAAAAAAAAAAAAAAAAAAAAA831220.2884688630654389No Hit
AAAAAAAAAAAAAAAAAAAA816410.28332916014443227No Hit
AAAAAAAAAAAAAA796360.27637095328648603No Hit
TGCCGTCGGGTCCCGGGG790650.2743893392635996No Hit
AAAAAAAAAAAAAAAAAAAAAA787250.27320939396100524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA776350.2694266281379821No Hit
AAAAAAAAAAAAA767560.2663761224880396No Hit
AAAAAAAAAAAAAAAAAAAAAAAA763100.26482831188522465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA734520.2549098304887108No Hit
AAAAAAAAAAAA724520.2514394031281391No Hit
ACCCCCACCACCACGCCCGCGGT723390.2510472448363945No Hit
TCGGCGGGAGAGGCCGGGAGG723290.25101254056278877No Hit
ACGCTCCCCGGACCCCGTCCCGGC715860.24843401303388402No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA712270.24718812961143882No Hit
TGGACCCGTCCCCCCGAC712240.2471777183293571No Hit
AAAAAAAAAAA683080.2370579521459301No Hit
GGACCCGTCCCCCCGAC677370.23507633812304365No Hit
GGGAGAGATCC673960.23389292239308873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA667470.2316406150360777No Hit
AGGAAGACGAACGGAAGGACGGAC642810.22308254116490794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA639110.22179848304149644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA638750.22167354765651584No Hit
ACGCTCCCCGGACCCC592710.20569570008844382No Hit
CCCCCGGTGGCGGCCCG591680.20533824607030499No Hit
AGGAAGACGAACGGAAGGAC583610.2025376111903236No Hit
AAAAAAAAAA563370.19551346621252655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550760.19113725731084566No Hit
TGAACGGGGAGGAGGCGGGAAC547540.19001977970074158No Hit
GGGAGAGCGACGCTC542330.18821168704588373No Hit
GGGAGAAAGCCC535280.1857650357566807No Hit
AGAAGGACTTGGGCCCCCCAC533510.18515077011385953No Hit
CCCCGGTCGCCGCGGT504960.17524269999942738No Hit
TGGGAGAGCGACGCTCAGAC465570.16157268662613555No Hit
AGGTCGACCCGTGCGGAGGAGC454050.157574754306757No Hit
TGGGAGAGATCC444600.15429520045101675No Hit
AAAAAAAAA444310.15419455805756016No Hit
TGAAGGGGGGACGGAGAAGGC437380.151789551896684No Hit
TAAGCTTGGGCTGC416830.14465782367070917No Hit
AGGAAGAC416760.1446335306791852No Hit
CCCCGGTGGCGGCCCG399120.13851169681513675No Hit
CGGTGGCGGCCCGGCGTCCGGC389340.13511761885649765No Hit
TCCCCGGTGGCGGCCCG376020.1304950096122162No Hit
GGGGAGAGATCC372570.12929771217281893No Hit
GGCGGGAGAGGCCGGGAGG369200.12812817815230632No Hit
AGAAGGACTTGGGCCCCC364740.12658036754949134No Hit
TGGCGGGAGAGGCCGGGAGG362400.12576828754711755No Hit
TAAGCTTGGGCTGCAGGTCGACCCGTG362190.12569540857254557No Hit
TCGTCGGGTCCCGGGG361680.1255184167771564No Hit
ACAAGCTTGGGCTGCAGGTC355680.1234361603608134No Hit
TGTCCCGGTCGCCGCGGTTC323720.11234467451642631No Hit
GGGGAGAGCGACGCTCAGAC319890.11101550083732736No Hit
AAAACCACGCTCCCCGGACCCCG318180.1104220577586696No Hit
AAAAAAAA313160.10867990322366262No Hit
GGAAAAAAAAAAAAA311300.10803440373459629No Hit
GGAAAAAAAAAAAAAAA305690.10608749398531558No Hit
GGAAAAAAAAAAAAAA305120.10588967962576298No Hit
GTCCCGGTCGCCGCGGTTC300550.10430369432198174No Hit
CCGGTGGCGGCCCGGCGTCCGGC292000.10133647892869295No Hit
GGAAAAAAAAAAAA290830.10093043892750607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)