FastQCFastQC Report
Tue 11 Jul 2023
SRR8495834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8495834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17119013
Total Bases504.9 Mbp
Sequences flagged as poor quality0
Sequence length20-290
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC16208529.468139313872827No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG14030768.196009898467862No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC8726875.0977646900554365No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8253094.821008080313976No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC8061934.709342764095103No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC3938612.300722594229001No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2996571.750433859709085No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC2218071.29567633367648No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC2104311.2292239044388833No Hit
CGACTGCATAATTTGTGGTAGTGGGG2074031.2115359688084821No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1948551.1382373504827645No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1936471.1311808688970562No Hit
CAATTTTTGACAGTCTCTACGGAGACTG1730311.0107533652787108No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC1677770.980062343547493No Hit
ACTGCATAATTTGTGGTAGTGGGGG1658260.9686656584699129No Hit
ACTGCATAATTTGTGGTAGTGGGG1636360.9558728648666837No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1493800.8725970358221002No Hit
GCAATTTTTGACAGTCTCTACGGAGACTG1167360.681908472176521No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC1161980.6787657676292436No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC1149110.6712478108404965No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1132560.6615801974097456No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG1098440.6416491418050795No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT809900.4730997049888332No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG755470.4413046476452819No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC744450.43486736063580295No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG737780.4309711079721711No Hit
CTGGCAATTTTTGACAGTCTCTACGGAGACTG687760.40175213372406454No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG575880.33639789864053493No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG510890.29843426136775525No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC507080.29620866576828936No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG504630.29477750849304224No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG459590.2684675804615605No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG446460.26079774575788917No Hit
AATTTTTGACAGTCTCTACGGAGACTG440630.2573921755886277No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC434010.25352513021632733No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCG427960.24999104796520685No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT402090.23487919543025057No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA316280.1847536420470035No Hit
AACGATGCCGACCGGCGATGCGGCGGCG308620.18027908501500642No Hit
GCATAATTTGTGGTAGTGGGGG305350.1783689281619215No Hit
ACTGCATAATTTGTGGTAGTGGGGGA293610.17151105615726794No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG290330.1695950578459167No Hit
GCATAATTTGTGGTAGTGGGG280730.1639872579102545No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG267750.15640504508057795No Hit
CGCTTCTGGCGCCAAGCGCCCGGC260540.152193353670565No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA253210.14791156476135628No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG242840.14185397253918786No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGT225680.13183003015419173No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC212920.12437632940637408No Hit
GACCTGGTATTGCAGTACCTCCAGG208850.12199885589198396No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC207740.12135045402442302No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC205980.12032235736955163No Hit
TCGGTTGGCCTCGGATAGCCGGTCCC196170.11459188681029683No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC195710.11432317973004635No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC192110.11222025475417303No Hit
TCACTGACCCGGTGAGGCGGGG189270.11056128060653965No Hit
TCGACTGCATAATTTGTGGTAGTGGGG183640.10727253960260442No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC182790.10677601564996768No Hit
CCCTGGTGGTCTAGTGGTTAGGATTC175520.10252927549035684No Hit
GACTGCATAATTTGTGGTAGTGGGG173340.10125583758830023No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG173150.10114484988124023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)