FastQCFastQC Report
Thu 28 Sept 2023
SRR8535409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8535409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27088203
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA6971962.5737993767988225No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA4768981.7605376037679576No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3253861.2012092496501152No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT1476060.5449087929531538No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC1426230.5265133312829944No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG1054810.389398292681135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTCTGAACT922900.34070181768794333Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTCTGAACTCC920450.33979736492671736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG856750.3162815931348417TruSeq Adapter, Index 7 (100% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG701520.2589762045123481No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGAAGAGC676420.24971017826468592No Hit
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACA661180.24408411292546794No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC658510.2430984439979278Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA639240.23598464615759118No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT631780.23323067979075612No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCAGATCGGAAGAGCACACGTCTGA614410.22681829429586006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACT564970.20856680673871203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGCTGGGCCTCGATCAGAAGGACAGATCGGAAGAGCACACGTCTGAA532510.19658373056344858Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA485270.1791444046694423No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTG473130.1746627489464694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGA455990.1683352712618109No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAA424440.15668813468357423No Hit
AGAGGCGGGCATGACACAGCAGATCGGAAGAGCACACGTCTGAACTCCAG416880.1538972518775055Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCCTTAGCGGATTCCGACTTCCAGATCGGAAGAGCACACGTCTGAA410780.15164534908424895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGTAGATCGGAAGAGCA405200.14958541177500775No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACG403710.1490353568304254No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGATCGG385020.14213567433764432No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGCAGATCGGAAGAGCACA372690.13758387738012742No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACA362280.13374087605589785No Hit
GACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCA334530.12349656416854231Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGGCTCCGGGACGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT328310.1212003616482053Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTC316440.11681838031116351No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCA315090.11632000838150837No Hit
GGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCTGAA312910.1155152300062134Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA311650.11505008287186862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTG309780.11435974545819817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAG301340.11124399798687273No Hit
CAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCAC299300.11049090262650499No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCACACG294060.10855648121065838No Hit
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA290580.10727178912532515Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCTGAAC287460.10611999622123329Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAAG286490.10576190676066625No Hit
CGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACACGTCTGAACT283060.10449567289494988Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG282590.10432216563055142No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT277670.10250587682025271No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCACAGATCGGAAGAGCACA276800.1021847037989194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)