FastQCFastQC Report
Thu 28 Sept 2023
SRR8535410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8535410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56873900
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG10647311.8720907129632396TruSeq Adapter, Index 10 (100% over 49bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA5018630.8824135499763512No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3551350.624425263609494No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA2403140.42253828205908156No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTG2221790.390651951070702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGA1452520.25539307133852257No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAA1436190.2525218070151687No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCA1272440.22373004137222877No Hit
ATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTCTGAACTCC1176620.20688224299722718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGTAGATCGGAAGAGCA1175380.20666421680243485No Hit
CGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGT1034630.18191648541773994No Hit
CGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC893370.15707908196905787Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGCAGATCGGAAGAGCACA872890.15347813320345535No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAAG855320.15038884268530908No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACG831320.14616898085061863No Hit
AGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGATCGGA753590.13250190333351503No Hit
CCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACG722150.12697388433007056No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGAAGAGC695520.12229159596932865No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGAA673470.11841459790870681No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA640400.11259997995565628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT637800.11214282825689816Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC628060.11043026766231961Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT620380.10907991187521868No Hit
ACCGGCTCCGGGACGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT593540.10436069972342321Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGATCGG577820.101596690221701No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACAC575660.10121690265657884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)