FastQCFastQC Report
Thu 28 Sept 2023
SRR8535411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8535411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29266871
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4102261.4016735851263362No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3654341.2486268176738127TruSeq Adapter, Index 1 (100% over 49bp)
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGA2776710.9487553349997682No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAA2659760.9087954773163144No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCA2471970.8446307772361452No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA2021600.6907468857876881No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAAG1542270.5269678470240293No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGAA1254430.4286177364160316No Hit
TTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGG1174450.4012899089895876No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC1113800.3805668190494296No Hit
AGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGATCGGA782280.2672919834853545No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA743790.25414059466760214No Hit
TTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGA658940.22514876974719986No Hit
TAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATCG628950.21490168867044243No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAGATC625010.21355545661167538No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAG622640.21274566727683325No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC591920.20224915741761393No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCA565030.19306129445816056No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGC541850.18514107640683555No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGC540740.18476180798418798No Hit
AGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGAT534960.18278687872031144No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACAC496950.16979949786910942No Hit
CGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGA443530.1515467779251154No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGAT421440.14399899463116503No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGAAGAGC416000.1421402376769283No Hit
ACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCATAGAT408980.13974162116613012No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGA407280.13916075961793115No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTG398530.1361710310610246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGATCGG376160.12852757645325324No Hit
CTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATC360410.12314606505082146No Hit
CGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGT346320.11833174786604281No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACACG334780.11438872300356263No Hit
AACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAG334680.11435455467719799No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGCAGATCGGAAGAGCACA327280.11182609852621417No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCAGATCGG324340.11082154973109355No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGTAGATCGGAAGAGCA322960.11035002682726144No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG322010.11002542772679731No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCAC321120.10972132962215196No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA315450.10778398551727653No Hit
AATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAGATCGGA302370.10331476842878079No Hit
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGC301460.10300383665886252No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCTAGATCGGAAGAGCA300970.10283641185967574No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA299650.10238538995166241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)