FastQCFastQC Report
Thu 28 Sept 2023
SRR8535412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8535412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40561915
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA20811885.130891872338868No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10462532.579397447087989No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA9813372.419355693635273No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC4292601.0582833675382437No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT4274611.0538481726023043No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA2866780.7067664334881625No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCAGATCGGAAGAGCACACGTCTGA2683390.6615540711033984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT2416500.5957558956474318No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGAAGAGC2056580.5070224125266275No Hit
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACA1817780.4481494525098236No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTG1800370.443857248850307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG1730520.4266366615087083No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGATCGG1725460.42538918589026187No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1558380.3841978368131781No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACG1351690.33324116970315626No Hit
AAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACT1327300.3272281399928973Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGTAGATCGGAAGAGCA1282810.31625972294454047No Hit
AGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTCTGAACT1248730.307857752771288Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGACCGACTGACCCATGTTCAACTGCTGTTCACAGATCGGAAGAGCACA1210900.29853127003495766No Hit
AGAAGGACTTGGGCCCCCCACGAGCAGATCGGAAGAGCACACGTCTGAAC1065710.26273660895941425Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGAAGAGCACAC1058220.26089004920009323No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGA1039600.2562995361535569No Hit
ACGGGCTGGGCCTCGATCAGAAGGACAGATCGGAAGAGCACACGTCTGAA1029180.25373062391161755Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAGGGC1010550.24913764549824632No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGCAGATCGGAAGAGCACA1002030.24703715295493323No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACAGATCGGAAGAG969460.23900745317374686No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCAGATCGGA957040.23594546756483267No Hit
AAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGA940510.23187021618678505No Hit
ATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTCTGAACTCC869460.2143537848250015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCAGATCG862380.2126083051059103No Hit
AAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCAG853510.2104215247233766No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCAGATCGGAAGA849340.20939346675323392No Hit
AGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGA809820.19965033702180973No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAGATCGGAA800790.19742411076991806No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAA785360.1936200497437066No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT763650.188267738345194No Hit
ATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAG739870.18240509601186236No Hit
AGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCAGATCGGAAGAGC738250.18200570658461268No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCA713770.1759704885728398No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACAGATCGGAAGAGCA705000.17380836185865486No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG675050.1664245881882056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGA656840.16193515518189908No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG635150.1565877745170562No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC632180.15585556056709848Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCAGATCGGAAGAGC626600.1544798858732385No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCA626160.154371409732504No Hit
AGAAGGACTTGGGCCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT623570.1537328797222715Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGATCGGAAGAGCAC607480.14976610448495836No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC599840.14788256422311422No Hit
ACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCG577510.14237740008083938No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACAC569880.1404963251858301No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA550390.13569132522465965No Hit
AGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCAGATCGGAAGAGCAC541510.13350207947529105No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACAC539490.1330040753746464No Hit
ACACTCCTTAGCGGATTCCGACTTCCAGATCGGAAGAGCACACGTCTGAA523530.12906934990618663Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGCCACCGTCCTGCTGTCTATATCAACCAACAGATCGGAAGAGCACAC520980.12844068136329362No Hit
AATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAGATCGGAAGAGCACA517240.12751863416705056No Hit
GGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCTGAA513850.12668287481002807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGAT513190.12652016059892637No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACA509920.1257139856439224No Hit
CGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACACGTCTGAACT502950.12399562496001482Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTC501170.12355678966340716No Hit
AACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAG496660.12244490922087875No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGA495720.12221316473840053No Hit
AAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATAGA488040.1203197630092169No Hit
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC485020.11957522222508479Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA479650.11825132023475717Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAAC479320.11816996312920629Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACACGTCTGAACTC475870.11731941157117458Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGATCGGAAGAGCA472770.11655514785236347No Hit
AGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCAGA468750.11556407038474392No Hit
GACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCA462110.11392706680638721Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAGATCGGAAG461700.11382598676615736No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTG460200.11345618174092618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGATCGGA459270.11322690262528284No Hit
ACGGAATCGAGAAAGAGCTATCAATCTGTCAGATCGGAAGAGCACACGTC447810.11040159223251664No Hit
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGC443890.10943516843324583No Hit
ACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGCACACGT441520.10885087649338054No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG440640.10863392421191159TruSeq Adapter, Index 3 (100% over 49bp)
ATCTCTCAGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTG440260.10854024027218635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGACATTCAGAGCACTGGGCAGAAATCAGATCGGAAGAGCACACGT439930.1084588831666355No Hit
CAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCAC433840.10695747476419691No Hit
TTGGTATACAACCCCCCACTGCTAAATTTGACAGATCGGAAGAGCACACG428490.10563850350753902No Hit
CGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGAACT412660.10173582780793264Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCA409050.10084583038054293Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)