FastQCFastQC Report
Thu 28 Sept 2023
SRR8535423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8535423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57984096
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG12279022.117653088874577TruSeq Adapter, Index 15 (97% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10119081.7451474969964176No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA8229381.4192477882211012No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTG3957450.6825061134004745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA3605590.6218239566932284No Hit
CGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3151390.5434921327392945Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGATCGGA2342060.4039142043363063No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACAC2252490.3884668651210842No Hit
CGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGT1924530.3319065283004498No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAA1691460.2917110236572456No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC1680950.2898984576736352No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGA1526330.2632325250013383No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACACG1446020.24938217541582436No Hit
ATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTCTGAACTCC1443280.24890963204807054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1423980.2455811331438193Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGATCGG1414070.243872043810082No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGAAGAGC1393730.24036418537938406No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACG1380020.23799974392978376No Hit
CCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACG1374970.23712881546001854No Hit
AACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAG1339070.23093746257594497No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGTAGATCGGAAGAGCA1327490.2289403632333942No Hit
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGC1296430.22358372199163026No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC1207880.20831229308119248No Hit
AGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGAT1194740.20604615444897167No Hit
AGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCTGAAC1148270.19803188791630036Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCATAGAT1082200.18663738415444125No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGCAGATCGGAAGAGCACA1082190.18663565954361003No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCA1056570.18221720659402882No Hit
AAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCA987310.17027255197701108No Hit
ACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAGA956500.16495902600602758No Hit
ACGGGCTGGGCCTCGATCAGAAGGACTAGATCGGAAGAGCACACGTCTGA921970.15900394480583088Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAGATCGGAA912060.1572948554720936No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAAG887330.1530298928864908No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT859870.14829411154396543No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGATCGGAAG847500.14616076794574845No Hit
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGT834610.14393774458430808No Hit
AGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGA820240.14145947881984744No Hit
AAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGATC813930.1403712493853487No Hit
AAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATAGA791670.13653226567505683No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAGATC788570.13599763631737918No Hit
AAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGATCGGAA787640.13583724751007586No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACA777550.13409711518137662No Hit
AAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTAGATCGGAA757900.13070825489803273No Hit
ACGGGCTGGGCCTCGATCAGAAGGACAGATCGGAAGAGCACACGTCTGAA754750.13016500248619897Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCAGATCGGA754220.1300735981121444No Hit
AAAGGATTTAAAGTGGACTCATTCCAATTAGATCGGAAGAGCACACGTCT752570.1297890373249934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAGATCGGA744490.12839555177336903No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCA741330.12785057475070405No Hit
CGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAGAT729820.12586554768397185No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC708510.12219040200264568No Hit
CGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGA698670.1204933849447269No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCG681330.11750290976339443No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCTAGATCGG675670.11652678003292488No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG674320.11629395757071041No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA662610.11427443828735383Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAGA659050.11366047683144014No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGAA654680.11290682189819773No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTAGATC633720.10929203759596423No Hit
AACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTAGATCGG627620.1082400249889211No Hit
ACTTTGACATTCAGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAA627530.10822450349144014No Hit
AAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAGATCG606030.10451659020432086No Hit
TAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATCG596960.10295236818040589No Hit
CAGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGT595800.10275231332398456No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTAGAT585630.10099838410863558No Hit
TTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGG582100.10038959648521553No Hit
ATGGCCACCGTCCTGCTGTCTATATCAACCAACAGATCGGAAGAGCACAC580180.10005847120562161No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)