FastQCFastQC Report
Thu 28 Sept 2023
SRR8535424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8535424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29670410
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG5228011.7620282294717196TruSeq Adapter, Index 3 (100% over 49bp)
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC5146261.7344755262903344No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGAT3151761.062256975889447No Hit
CCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGA2708350.9128117879058631No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGAGA2375900.8007641283015638No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2334180.7867029811856324No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGAT2309110.778253485543341No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCA2120540.7146985835382794No Hit
CGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGAGATCGG2116720.7134111055425254No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGA2050150.6909746107317021No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAGATC1839270.6199004327880875No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG1817080.6124216011844797No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGAGAT1777660.5991356371549972No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAA1653850.5574071945753362No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGATC1644370.5542120921146692No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA1510970.5092514731006413No Hit
GTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGAGATCGGA1416960.4775667070323598No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1322720.44580442265543346No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCA1301860.4387738490974678No Hit
CGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGATCGGA1204260.40587912334207715No Hit
CCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAG1150970.38791846826518406No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTAGATC975700.3288461467165435No Hit
TTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGG945180.31855980419549307No Hit
TAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATCG856770.28876244042465204No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGAG844250.28454274814537445No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA828900.2793692436336404No Hit
CGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGA778060.2622343270618775No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAGATCGGAA772060.2602121103146198No Hit
AGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGATCGGA769970.2595077048143251No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGA767550.2586920773929312No Hit
TGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGAAG750620.2529860558044193No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGA709730.23920464867185862No Hit
AGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGAT669150.22552772273790622No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGAG651910.21971721995078597No Hit
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGC632110.21304390468483586No Hit
AAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGATC596470.20103193720612555No Hit
ATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCA572980.19311495864061196No Hit
CTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATC572260.19287229263094105No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCAGATCGG541180.18239720988014657No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGAGA534880.1802738822955261No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGATCGG515790.17383986267800142No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGATC515310.1736780853382208No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGA513450.17305119814657094No Hit
TTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTAGATCGGA497940.16782376785491No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGA493700.16639473468684796No Hit
AACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAG491960.16580829183014323No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTG491160.16553866293050887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAGATCGGAAGAGC488380.16460170250427952No Hit
GTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGATCGGAA482280.1625457821445676No Hit
CGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATCG464770.15664427960382077No Hit
AAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCA442510.149141855471495No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA430340.14504012583580744No Hit
ACTTTGACATTCAGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAA404940.13647940827241686No Hit
AAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATAGA387720.1306756462077875No Hit
AGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGA383220.12915898364734427No Hit
CGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAGAT371960.1253639568849908No Hit
CGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGAGATCG371550.12522577207392818No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGATCGGAAG362120.12204752141948831No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA356680.12021404490197474No Hit
CCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAGA348730.11753460771185838No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGATCG343730.1158494270891437No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAG340850.11487876305046005No Hit
ACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCATAGAT338920.11422828333009216No Hit
ACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGATCG335140.11295428677931987No Hit
ACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAGA333290.11233076994891543No Hit
TGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAG330310.11132640229777747No Hit
CGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGT327410.11034899753660297No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA322920.10883570533740518No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAGA321270.10827959573190933No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAGATCGGAA315420.10630793440333315No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGA314360.10595067611131764No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCTAGATCGGAAGAGCA300070.10113442989159907No Hit
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC298020.10044350583628604Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)