FastQCFastQC Report
Thu 28 Sept 2023
SRR8543469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8543469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47779609
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG9974702.087647891802547TruSeq Adapter, Index 5 (100% over 49bp)
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAG9304431.9473641988154402No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC8560121.7915843555772926No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGA3368360.7049785610426406No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAA2440720.5108287930945605No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGA2412870.5049999467345997No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTC1928200.4035612765269803No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC1885260.39457417912314857No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGA1703720.35657889121696246No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGC1625590.3402267272635069No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAG1483080.3104001960334167No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1420120.2972230266681337No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATCG1334250.2792509248035077No Hit
CGTGTCAACTATAAATAAATAAATAGAATTAAAATCCAACTTAGATCGGA1247960.26119091933129884No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAGATCGGAAGAGCACACGTC1192770.24963996670629932No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA1073990.2247799893046425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAATCCACCTATAACTTCTCTGTTAACCCAAAGATCGGAAGAGCACACGT1072770.22452465025404456No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG949750.19877726500440807No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGA874390.18300484627239205No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA786550.16462043462934156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAATCCACCTATAACTTCTCTGTTAACCCAGATCGGAAGAGCACACGTCT774370.1620712300094377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA768730.16089081013618173TruSeq Adapter, Index 5 (100% over 36bp)
ACGTGTCAACTATAAATAAATAAATAGAATTAAAATCCAACTTAGATCGG730330.1528539088714602No Hit
TAATCCACCTATAACTTCTCTGTTAACCTAGATCGGAAGAGCACACGTCT706590.14788526209998915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCG691690.14476677697383417No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAT683770.14310916608798538No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATC680170.1423557065944177No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAGATCGGAAG633940.13268003093118655No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTAGATCG601660.12592401080553003No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGAG578610.12109977710365943No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC554700.11609555030054766No Hit
CGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGA530740.11108085878224748No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA496260.10386439118829958No Hit
GACCGGCTCCGGGACGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAG484630.10143029843546858Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)