FastQCFastQC Report
Thu 28 Sept 2023
SRR8543470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8543470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29764486
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC9521903.1990809449892734No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAG3621781.2168125463345816No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAGATCGGAAGAGC1800690.6049793703811985No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1708610.5740431734651826TruSeq Adapter, Index 9 (100% over 49bp)
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAA1578560.5303501629425081No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTC1466080.4925601604543079No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1303790.4380354493606911No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATCG1016440.3414942223426939No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGC988560.3321273547273755No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAGATCG754560.2535101731640856No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGA719140.24161008525394995No Hit
TAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATCG655610.22026585643037813No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC650520.21855576474594587No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGA647580.21756801041348403No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC632290.21243101594295966No Hit
CGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGA598910.20121630859004253No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGA548540.1842934563022523No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTAGATCG535400.1798787991836983No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAG533540.17925389338152858No Hit
AATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTCT523600.17591434301939568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATCCACCTATAACTTCTCTGTTAACCCAGATCGGAAGAGCACACGTCT478560.16078221542276927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG470480.1580675708628061No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGA468930.15754681602766465No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATC413540.1389373900157389No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATCGGAAGAGCACACG386500.12985273792398094No Hit
CTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGCA386200.12975194666556647No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC359940.12092935184568616No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA350860.11787873642434141No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAG349640.11746885197345588No Hit
CTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATC343780.11550006272575983No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCG333180.11193877159511507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)