FastQCFastQC Report
Thu 28 Sept 2023
SRR8543471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8543471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27577648
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG11312144.101923412758042TruSeq Adapter, Index 5 (100% over 49bp)
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAG3301701.197237704970344No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAGATCGGAAGAGC1854860.6725954294579437No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAGATCG1099030.3985220204420624No Hit
AACGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTCTGAAC1068710.3875276093160664Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1057690.3835316195202723No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAA976780.3541926418090477No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA929000.336867016360496No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTC924310.3351663637160065No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC881040.31947612066119635No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGC874660.317162652884684No Hit
ACGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTCTGAACT782970.2839147123786626Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTCT766980.2781165384372155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG754350.27353674250973103No Hit
CGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTCTGAACTC721000.2614436154961438Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA685980.24874492560061687No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC637820.23128150740048606No Hit
TAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATCG598540.21703808823725648No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGA596620.21634187222927784No Hit
CAACGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTCTGAA593470.21519964284118792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATCG572560.2076174153792956No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGA563550.20435027671685418No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG522390.18942514604581218No Hit
CGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGA501800.1819589545852496No Hit
AAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAGATCGG476330.17272321410440802No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGA452380.16403864462988285No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGA437320.15857770031730045No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG437220.1585414390668849No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATCGGAAGAGCACACG436710.15835650668976556No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAGATCGGAAGAGC402930.14610745629939145No Hit
AATCCAACGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTC390870.14173434949927563No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTAGATCG383860.13919243584514532No Hit
CTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGCA367100.13311505027549847No Hit
AGAGCCGACATCGAAGGATCAAAAAGCGACGTAGATCGGAAGAGCACACG359020.1301851412419217No Hit
CAAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAGATCG348110.1262290388215848No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG329470.11946994174412554No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGATCGGAAG307040.11133654327591677No Hit
CTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATC306970.1113111604006259No Hit
CCAACGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTCTGA300150.10883814312228512Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA290290.1052627838313115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)