FastQCFastQC Report
Thu 28 Sept 2023
SRR8543473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8543473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27837164
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC8541993.0685561215934207No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAG4905511.7622161510418228No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG4663991.6754544392525044TruSeq Adapter, Index 3 (100% over 49bp)
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAGATCGGAAGAGC2186570.7854859065384677No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTC1634230.5870677056039186No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1585210.5694581531365767No Hit
CGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGA1493790.5366171640185761No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAGATCG1425740.5121714266582615No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAA1416070.5086976532523212No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC1400430.5030792648274085No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATCG1284920.46158437691425747No Hit
TAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATCG1277330.45885780606099097No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGA1021030.3667866453637303No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG993010.3567209648224223No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGC972260.3492669008955079No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGA812560.2918975510580029No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGA710720.25531336453670356No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATC666390.23938861013284257No Hit
CTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATC632060.22705617569375958No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAG630200.2263880041803109No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAG606180.2177592516249141No Hit
CAGAAGGACTTGGGCCCCCCAAGATCGGAAGAGCACACGTCTGAACTCCA584870.21010401777997212Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGA582520.2092598225882493No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC533490.19164667780094266No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG502150.18038834703132833No Hit
CGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATCG499340.17937890512122573No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA490880.1763398024310235No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCG483880.17382517845567888No Hit
AGAAGGACTTGGGCCCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG467460.16792658907351338Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAGATCGG457720.16442766942781958No Hit
TAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAGA452850.1626782096049727No Hit
CGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCAGATCGGAA433370.15568037031358511No Hit
AGAGCCGACATCGAAGGATCAAAAAGCGACGTAGATCGGAAGAGCACACG412710.14825863726635372No Hit
AATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTCT406060.14586974448977633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAAAGAT374420.13450364412121868No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAG357420.12839670018109603No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTAGATCG354450.12732978115155696No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAGATCGGAAGAGCACACGTCT344950.12391707718501783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGAAC341760.12277112711625365Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG308900.11096676371199307No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATCGGAAGAGCACACG306590.11013693780012936No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA299340.10753250582566527No Hit
CTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGCA296270.10642966359647843No Hit
CGACATCGAAGGATCAAAAAGCGACGTAGATCGGAAGAGCACACGTCTGA293090.10528730584767902Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCA290440.10433534105701286No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAGATCGGAAGAGC287750.10336900698648757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)