FastQCFastQC Report
Thu 28 Sept 2023
SRR8543476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8543476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32481458
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC30945739.527198563562017No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAG19048105.864299564385318No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG5674451.7469813085360886No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAA5224321.6084007066431563No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC5131821.5799229209476988No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG4511941.3890817339541839TruSeq Adapter, Index 7 (100% over 49bp)
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGA4354651.3406571835537677No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG3860151.1884164805656199No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATCG3586451.1041530217024125No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTC3239130.9972243241051556No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGC3085670.9499789079665082No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAGATCGGAAGAGC2748040.8460334508383214No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGA2680180.8251415315162269No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGA2191180.6745941022721332No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGA1959900.6033904019948858No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATC1743610.5368016423400698No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAGATCG1609700.4955750446916514No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCG1589300.4892945384409776No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1567670.48263535460754253No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC1492310.45943442563446507No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGAT1259950.3878982279674761No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAG1251190.3852013046951279No Hit
CGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATCG1169900.3601747187580065No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAG1072470.3301791440519696No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTAGATCG1019240.31379133288905936No Hit
AATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTCT958740.2951653217044629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATCCACCTATAACTTCTCTGTTAACCCAAAGATCGGAAGAGCACACGT955110.2940477610333871No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGG913170.28113577906508996No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAGATC909270.2799350940465788No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAGATCGGAAGAGCACACGTCT908540.27971035044054976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAGATCGGAA903850.2782664497388017No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAG871300.2682453478535354No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA870970.26814375142889213No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGAT863480.26583782045744375No Hit
CTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGCA829940.2555119292982476No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA810120.24940998646058316No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGAAC809480.24921295097036592Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATCGG791190.24358204610150197No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG786240.2420580997318532No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATCGGAAGAGCACACG763410.23502947435426083No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAGATCGGAAGAGC749980.23089480773923385No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAACAGATCGGAAGAGCACACG743430.22887827264404204No Hit
CGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGATCGGA741430.22826253673711322No Hit
TAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAGA733000.2256672098894083No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAGATCGGAAG684110.21061554564453358No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA654660.20154883441500684Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGAG646840.19914130701891522No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAGATCG561610.17290172134514406No Hit
TCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAG556010.1711776608057434No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGTCTGA547000.1684037705450291Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGATCGGAA524120.16135975176976355No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC482840.14865096265075292No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGATC464310.14294616947305752No Hit
CGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACTC446120.13734605139954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA442360.13618846789451383TruSeq Adapter, Index 7 (100% over 37bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAAGATC437250.13461526265231075No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAAAGAT427760.13169359577393355No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA394360.12141080612822246Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAGATCGGAAGAGCA384570.11839677886380594No Hit
CGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGTCTGAACT382790.1178487739066393Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAGATC382110.1176394236982835No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAGAT372150.11457305888177803No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA364540.1122301837559139No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG358600.11040144811233536No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAGATCGGAAGAGCA345570.10638992867869418No Hit
ATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCA335460.10327738366916904No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT334890.10310189893569432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)