FastQCFastQC Report
Thu 28 Sept 2023
SRR8543477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8543477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26887138
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG26082029.700556451936237TruSeq Adapter, Index 1 (100% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5706122.122248935531926No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAG2673340.9942820987492236No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1373490.5108353295170353No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAA1245660.4632921510649441No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTC1102240.4099506611674325No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAGATCGGAAGAGC949020.352964305832774No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATCG847050.31503910903421556No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAGATCG801700.2981723082612958No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTAGATCG795590.2958998462387481No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCAGATCGGAAGAGCACAC789480.2936273842162003No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGA690120.25667291178406565No Hit
TCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGC683610.2542516797436752No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC574030.21349613335565876No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAAG563530.20959092038728705No Hit
CTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATCGGAAGAGCACACG549290.20429470775208575No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGA539520.20066100006627705No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC477970.17776901357072664TruSeq Adapter, Index 1 (100% over 50bp)
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGA461190.17152811132222404No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAGATCGGAAGAGCACACGTCT441810.16432020395774366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACATCACGATCTCGTATG417420.15524895211978307TruSeq Adapter, Index 1 (97% over 49bp)
TTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAATAG363320.13512780720655357No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAGATC362940.13498647568960298No Hit
AGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCG343060.12759260580281917No Hit
TTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGGAAGA337840.12565115706997151No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC334290.12433082316161727No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA327130.12166783984223237No Hit
CAAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAGATCG318950.1186254929773485No Hit
CTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGAAGAGCA315870.11747996383995947No Hit
AATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTCT313690.11666916724271657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATCCACCTATAACTTCTCTGTTAACCTAGATCGGAAGAGCACACGTCT297360.11059563126428704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATCCACCTATAACTTCTCTGTTAACCCAACAGATCGGAAGAGCACACG277350.10315341112170436No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG269420.10020404551797221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)