FastQCFastQC Report
Fri 14 Jul 2023
SRR8545522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15139154
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA5903753.899656480144135No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA3843622.5388604937898114No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1273850.8414274668188196No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG1241470.8200392175150607No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG1238860.8183152110084883No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC1170110.7729031622242564No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1168460.7718132730534348No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG1093740.7224578070875031No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA1083500.7156938888394953No Hit
ATACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC837770.5533796670540507No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC810880.5356177762641162No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA803160.5305184160224541No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC742720.4905954454258144No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA699230.4618686090385235No Hit
TTTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGA651990.4306647518084564No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA648840.4285840543005243No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA607820.40148874897500875No Hit
ACGGAAGTTAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGA543170.3587849096455456No Hit
ACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA512260.3383676525121549No Hit
ACGGCCCAGACTCCTACGGGAGGCCTGTAGGCACCATCAATAGATCGGAA505680.33402130660669677No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA482880.31896101988261694No Hit
ACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG482610.3187826743819371No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCG434090.28673332737086893No Hit
ACCCGGAGGAAGAGAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA433960.28664745731498603No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG428200.28284275330048164No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG424020.28008170073440036No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG396840.2621282536659578No Hit
AAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGATCGGAAGAG396100.2616394548863166No Hit
TAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCA390390.2578677778163826No Hit
ATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAG374430.2473255771095267No Hit
TTATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGA353190.23329573105604182No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA351230.2320010748288841No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA342740.2263930996408386No Hit
ACGGCCCAGACTCCTACGGGAGGCAGCCTGTAGGCACCATCAATAGATCG340590.22497294102431353No Hit
TAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG339340.22414726741005475No Hit
ACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC333120.22003871550550316No Hit
TAGGCTGGGAAATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGA319150.21081098719254723No Hit
ATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA319030.21073172252557837No Hit
ATAGGAGGTAACAGCCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGC303060.20018291642980843No Hit
AGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGGA301350.19905339492550245No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG287750.19007006600236712No Hit
ACAGGTCTCTGCGAAGCCGTAAGGCGAAGTCTGTAGGCACCATCAATAGA287340.18979924505689022No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCAT285590.18864330199692797No Hit
ATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAG283290.18712406254669184No Hit
ACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAG279990.18494428420504871No Hit
TCTGGTGATGATGGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA279320.184501723147806No Hit
TACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAA278160.18373549803377387No Hit
ATAACGGTCCTAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC274540.1813443472468805No Hit
ACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATCGGAAGA273040.18035353890977No Hit
AACACCCGAAGTCGGTGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG262920.17366888532873104No Hit
ACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAGAG253100.1671823934151142No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC252490.16677946469135593No Hit
ACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAGAGC251530.1661453473556052No Hit
ACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCCTGTAGGCACCA246810.1630276037881641No Hit
ACCGTGAGGGAAAGGTGAAAAGCCTGTAGGCACCATCAATAGATCGGAAG242180.15996930872094967No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA238420.15748568248925932No Hit
ACATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCA235750.15572204364920259No Hit
TAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGG233210.15404427486502878No Hit
ACACCACGAGAGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA222170.1467519255038954No Hit
ATACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCG220230.1454704800545658No Hit
TCTGGTGATGATGGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG210580.13909627975248814No Hit
AAATGCGATTCCCTGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC204500.13508020329273351No Hit
ACCGGATGGTTGTTTGAACCGCATGGTTCAAACATCTGTAGGCACCATCA202880.1340101302886542No Hit
AACTCCGGGAAACCGGGGCTCTGTAGGCACCATCAATAGATCGGAAGAGC202520.13377233628774765No Hit
TAGTCGGGGGTTTCCCCCTGTGAGAGTCTGTAGGCACCATCAATAGATCG200830.1326560255612698No Hit
AACACCCGAAGTCGGTGAGGTAACCTTCTGTAGGCACCATCAATAGATCG200140.13220025372619898No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA199240.13160576872393268No Hit
AGTCGGGGGTTTCCCCCTGTGAGAGTCTGTAGGCACCATCAATAGATCGG198770.13129531544497136No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG196360.12970341671668048No Hit
ACACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAG196310.12967038977211012No Hit
ACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC189970.1254825732005897No Hit
TATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAA189160.12494753669855001No Hit
AAGCACTCCGCCTGGGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGC180150.11899608128697284No Hit
ACGAAAGGCGCAACGATCTGGGCCTGTAGGCACCATCAATAGATCGGAAG177140.11700785922383775No Hit
ACACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC175510.1159311808308443No Hit
AAGGTGATCGAGAGAACTCTCGTTCTGTAGGCACCATCAATAGATCGGAA175120.11567357066319557No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGA173770.11478184315979613No Hit
AGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATCAATAGATCGGAA172580.11399580187902178No Hit
ACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGA172570.1139891964901077No Hit
ATGCTTCGGGGAGCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA171980.11359947854417757No Hit
ACACCCGTTCCCATACCGAACCTGTAGGCACCATCAATAGATCGGAAGAG171810.11348718693263839No Hit
TTTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA168960.11160465109212839No Hit
TTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG168290.11116209003488571No Hit
ATGCCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC168060.11101016608986208No Hit
AAGATCCCTGAAAGATGATCAGGTTGATCTGTAGGCACCATCAATAGATC167840.11086484753375254No Hit
ACGCGAGCTGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG162790.10752912613214716No Hit
ACGTTCCCGGGCCTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA162580.10739041296495166No Hit
ACGTGAAATTCCGTCGGAATCCTGTAGGCACCATCAATAGATCGGAAGAG161720.10682234951834164No Hit
AGATGAAGAGGTCTGGAAAGGCCCGCCATCTGTAGGCACCATCAATAGAT159030.10504549990045678No Hit
ATATCTGAATTCATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGAT155420.10266095450247748No Hit
AAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG154720.10219857727849256No Hit
TAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGATCGGAAGA153970.10170317310993732No Hit
TAGGGTGCAAGCCCGAGAGAGCCGCCTGTAGGCACCATCAATAGATCGGA152530.10075199710631122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)