FastQCFastQC Report
Fri 14 Jul 2023
SRR8545523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16969803
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA6868324.047377568260516No Hit
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA4661622.747008907528272No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG1555980.9169110566575227No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1543600.9096157450973354No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1403840.8272576882595515No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1348700.7947646769971343No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1273890.7506804881588784No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG1227000.723049053663145No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG1127730.6645510263142124No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA896850.5284975906909467No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA805480.4746548913973839No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC689720.406439603335407No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA677090.3989969712671385No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA637660.37576158073255184Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG622290.3667043158957119Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA616250.36314505242046713No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA548770.32338030087915576No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG535660.31565481343537105No Hit
ACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG534410.3149182108949644No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA481420.2836921560020467No Hit
ACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA427900.25215378163199653No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA415440.2448113275092233No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG403720.23790494209037075No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC398620.23489960372551172No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA396100.23341461300405197No Hit
AACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC372070.21925416576727497No Hit
AACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAG369690.21785167453034077No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC319980.18855846470345No Hit
ACGCGAAGAACCTTACCAGGTCTCTGTAGGCACCATCAATAGATCGGAAG306550.1806444070093212No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG294970.1738205210749942No Hit
ACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA274650.16184631017814408No Hit
TTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG263450.1552463514161007No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG260050.15324279250619469No Hit
TTTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGA241590.14236464618946962No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA239990.14142179493774912No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG228660.13474522951150347No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCG225270.1327475634219207No Hit
TTAAGGAGGTGTGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC223600.13176346242793743No Hit
ATACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC222220.1309502532233285No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA221820.1307145404103984No Hit
AAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGATCGGAAGAG205160.12089710175185886No Hit
CTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC203470.11990121511722912No Hit
AAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC199060.11730248135467453No Hit
ACGGAAGTTAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGA198950.11723766033111876No Hit
AAACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATAGATCG198860.11718462494820947No Hit
ATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAG194550.11464481938888743No Hit
TCAAGGAGGTGCGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC193510.11403196607526911No Hit
ACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC184860.10893467649565525No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGA177930.10485095201164091No Hit
TTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC174510.10283560746108839No Hit
ACAAACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAG171190.10087919111376838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)