FastQCFastQC Report
Fri 14 Jul 2023
SRR8545524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18470511
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA4773842.584573864794536No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA2884001.5614078029568321No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG1689290.9145875823359733No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1606040.8695157378158082No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1597490.8648867375677912No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1409050.7628646549085729No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1350360.7310896812762787Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1060600.574212592169215No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1052710.5699409182561327No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC1011490.5476242644288509No Hit
ACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA768630.41613900124365805No Hit
ATACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC738730.3999510354640432No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG725330.39269622805779436No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA705250.3818248450191768No Hit
AAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGATCGGAAGAG693740.37559329029933175No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG676990.3665247810415207No Hit
TTTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGA639580.34627087469317985No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG622010.3367584145343894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA621510.33648771276549955No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA607630.32897303166111647No Hit
ACGGAAGTTAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGA549440.29746875979771215No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCG544800.294956647382414No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC513990.2782760043834196No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG441450.2390025917528757No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA432180.23398378095765732No Hit
ACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG424020.22956592808937448No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA419150.22692929286038704No Hit
ATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAG414320.22431431377291075No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA404250.2188623801474686No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG370690.2006928774195798No Hit
ACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC358550.1941202384709335No Hit
ATACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCG352050.190601115475365No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA351500.19030334352958617No Hit
ATGAGTACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATC348700.18878741362380283No Hit
ATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA348600.18873327327002487No Hit
ACCCGGAGGAAGAGAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA339370.18373611861631764No Hit
TTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG333500.18055807984955044No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC308310.16692012473287826No Hit
ACGGCCCAGACTCCTACGGGAGGCCTGTAGGCACCATCAATAGATCGGAA308050.16677935981305553No Hit
ACGGCCCAGACTCCTACGGGAGGCAGCCTGTAGGCACCATCAATAGATCG298140.16141405075365808No Hit
TTTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA290300.15716944701746474No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA284470.15401306439220874No Hit
TTATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGA278660.15086750983770833No Hit
TAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG275230.14901049570312375No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA262450.14209135849029841No Hit
ACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC260210.14087861456567174No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG256350.13878879690984183No Hit
AAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC253840.13742987403001466No Hit
ACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATCGGAAGA246680.13355342469951156No Hit
ACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAGAG245900.13313112994004334No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG238730.12924926657416244No Hit
ATATCTGAATTCATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGAT233720.12653683484988584No Hit
TATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAA229230.12410593296525473No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA212410.11499952545979913No Hit
AACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC211510.11451226227579736No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG208040.11263359199970159No Hit
TCCGCCCCTTAGTGCTGCAGCTAACGCCTGTAGGCACCATCAATAGATCG206390.11174027616236498No Hit
ACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA201690.10919567953480008No Hit
TACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAA200290.10843771458190843No Hit
ATGTGGTTTAATTCGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA193660.10484820912642862No Hit
TCCGCCCCTTAGTGCTGCAGCTCTGTAGGCACCATCAATAGATCGGAAGA193250.10462623367593891No Hit
ACAGGTCTCTGCGAAGCCGTAAGGCGAAGTCTGTAGGCACCATCAATAGA192510.10422559505798189No Hit
ATAGGAGGTAACAGCCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGC191060.10344055992820123No Hit
TAGGCTGGGAAATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGA189830.10277463357673212No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCAT188770.10220074582668558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)