FastQCFastQC Report
Fri 14 Jul 2023
SRR8545525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26446929
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA6521452.465862860674674No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC3938541.489223947324848No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3510361.3273223518692852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2258680.8540424485580159No Hit
ATACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1852490.7004556181173247No Hit
ACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATCGGAAGA1794270.6784417200197422No Hit
TTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG1508680.5704556472322363No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC1467530.5548961847328285No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA1462030.5528165481897728No Hit
ATGAGTACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATC1445830.546691073281136No Hit
ATAGGAGGTAACAGCCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGC1375200.5199847589109495No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC1237110.46777075705084703No Hit
ACACCACGAGAGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1203740.45515303497052534No Hit
ATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA1199070.45338723448760343No Hit
ACAGGTCTCTGCGAAGCCGTAAGGCGAAGTCTGTAGGCACCATCAATAGA1165660.44075438777787773No Hit
ATACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCG1138130.4303448615905461No Hit
ACACCGCCCGTCACACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1106460.4183699362598962No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG1014780.3837042856658329No Hit
ATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAG976920.3693888239349076No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG967060.3656606027868113No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCG913750.34550325294857487No Hit
ACGGCCCAGACTCCTACGGGAGGCAGCCTGTAGGCACCATCAATAGATCG908340.34345764682167823No Hit
AAATGCGATTCCCTGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC898270.33965002136921074No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA894180.33810352801264754No Hit
AAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTCTGTAGGCACCATCA892250.3373737646439025No Hit
ACGAAAGGCGCAACGATCTGGGCCTGTAGGCACCATCAATAGATCGGAAG870290.3290703431010837No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA856930.3240187168801338No Hit
TTATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGA813850.307729491011981No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG765170.2893228170272624No Hit
TATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAA754640.2853412583366485No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC750080.2836170505845877No Hit
ACATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCA749590.2834317738743882No Hit
ATGCTTCGGGGAGCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA738560.27926115731622375No Hit
ATAACGGTCCTAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC681910.25784090092274986No Hit
ACCGATATGCTTCGGGGAGCTGTCTGTAGGCACCATCAATAGATCGGAAG678880.2566952102453937No Hit
ACACACCGCCCGTCACACCCTGTAGGCACCATCAATAGATCGGAAGAGCA677340.25611291201333813No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG674350.254982345965386No Hit
TACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAA632360.23910526624849335No Hit
ACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC604090.228415934417187No Hit
ACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC593330.22434740910749978No Hit
ACACCCGTTCCCATACCGAACCTGTAGGCACCATCAATAGATCGGAAGAG587960.2223169276100072No Hit
ACCCGGAGGAAGAGAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA581670.21993857963622165No Hit
ACTGGGACTGAGACACGGCCCCTGTAGGCACCATCAATAGATCGGAAGAG563710.21314762103380702No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG560550.2119527753108877No Hit
AACTCCGGGAAACCGGGGCTCTGTAGGCACCATCAATAGATCGGAAGAGC557730.21088648893790277No Hit
ACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAG545830.2063869116902004No Hit
ACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAGAG542930.20529037605840741No Hit
AGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATCAATAGATCGGAA539080.20383463047826839No Hit
ATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAG532810.20146384481918486No Hit
ACGCGAGCTGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG519860.1965672460496264No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA513980.1943439255272323No Hit
ACCTCCTCACCATTTGAGCAATGGGGGGACGCCTGTAGGCACCATCAATA500340.18918642690045412No Hit
ATGCTTCGGGGAGCTGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG481860.1821988481157869No Hit
AAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG478090.18077335179445597No Hit
AAGATCCCTGAAAGATGATCAGGTTGATCTGTAGGCACCATCAATAGATC476740.1802628955520696No Hit
ACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGA473880.1791814845496806No Hit
AAAACCAAGAGGCTTGCCTCTTGGGGTTGTCTGTAGGCACCATCAATAGA471510.17828535025749115No Hit
ACCGGGTTACCGAATTCAGTCCTGTAGGCACCATCAATAGATCGGAAGAG451620.1707646282863315No Hit
ACGTGTAGCGGTGAAATGCGTCTGTAGGCACCATCAATAGATCGGAAGAG450310.1702692966733491No Hit
AAGGCTGAGCTGTGATGGCGAGCGAAATCTGTAGGCACCATCAATAGATC448300.16950928404579602No Hit
ACGGCCCAGACTCCTACGGGAGGCCTGTAGGCACCATCAATAGATCGGAA447000.16901773359016467No Hit
AGATGAAGAGGTCTGGAAAGGCCCGCCATCTGTAGGCACCATCAATAGAT441210.16682844348392964No Hit
TAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG438720.16588693530352805No Hit
ACACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC436360.16499458216868962No Hit
TCTGGTGATGATGGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC433480.1639056088515986No Hit
ACCGGGTTACCGAATTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA431030.16297922530060105No Hit
AGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA430560.16280151090510359No Hit
AAGATCCCTGAAAGATGATCCTGTAGGCACCATCAATAGATCGGAAGAGC425470.16087690181343928No Hit
TCCGCCCCTTAGTGCTGCAGCTCTGTAGGCACCATCAATAGATCGGAAGA421600.15941359391859827No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC418990.15842671184998455No Hit
ACGAGAGTTTGTAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG416460.15747007904017893No Hit
ACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAGAGC415220.15700121552865362No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA412320.15590467989686063No Hit
ACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG410800.15532994397950703No Hit
ACGGGTTCGAATCCCGTACGGGTCACCACTGTAGGCACCATCAATAGATC400410.15140132149180724No Hit
ACCGTGAGGGAAAGGTGAAAAGCCTGTAGGCACCATCAATAGATCGGAAG399140.15092111450822893No Hit
ATCTGAATTCATAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA395720.14962795869418336No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA393770.14889063301073632No Hit
AAAACCAAGAGGCTTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACC385740.145854363657875No Hit
TCCGCCCCTTAGTGCTGCAGCTAACGCCTGTAGGCACCATCAATAGATCG384230.14528340889787242No Hit
AGTCGGGGGTTTCCCCCTGTGAGAGTCTGTAGGCACCATCAATAGATCGG384130.14524559732436232No Hit
ATATCTGAATTCATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGAT382610.14467086140700872No Hit
ACGGCTAACTACGTGCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGC382090.1444742412247562No Hit
TAGGCTGGGAAATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGA381280.14416796747932434No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCAT380640.14392597340885968No Hit
AACACCCGAAGTCGGTGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG377800.14285212472117273No Hit
ACCCGTTCCCATACCGAACCTGTAGGCACCATCAATAGATCGGAAGAGCA373500.14122622706023827No Hit
AACGCGAAGAACCTTACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC368530.1393469918567861No Hit
ACAGGTCTCTGCGAAGCCGTAAGGCGAAGTATCTGTAGGCACCATCAATA363630.137494224754791No Hit
ACACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAG362780.13717282637995512No Hit
AAGCGCGGTATTGGATATCCGCGTCCAAGCCTGTAGGCACCATCAATAGA355870.13456004665040694No Hit
AAGCCGAGGCCGAAAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGC355040.13424621059027309No Hit
ACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCCTGTAGGCACCATC352530.13329714009516946No Hit
ACGTTCCCGGGCCTTGTACACACCGCCCGTCCTGTAGGCACCATCAATAG346940.13118347313595466No Hit
ACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCCTGTAGGCACCA346400.1309792906390001No Hit
TAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCA324060.12253218511684286No Hit
ATCCTCTGACAATCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC323030.1221427259096888No Hit
TCTGGTGATGATGGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG323000.12213138243763577No Hit
ATTAAGCACTCCGCCTGGGGAGTCTGTAGGCACCATCAATAGATCGGAAG319960.12098191060292861No Hit
ACCGCCCGTCACACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG311170.11765827329139047No Hit
TAGGCTGGGAAATAGGCAAATCCGTTTCCCACTGTAGGCACCATCAATAG311130.11764314866198643No Hit
TCCGCCCCTTAGTGCTGCAGCTAACGCATTCTGTAGGCACCATCAATAGA310240.1173066256577465No Hit
ACCGCAAACCGACACAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCA304710.11521564564263775No Hit
ATTGGGCGTAAAGGGCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGC303560.11478081254727154No Hit
TACGGAGTTACAAAGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCA302210.11427035630488516No Hit
AGTCGGGGGTTTCCCCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGC298980.11304904248050879No Hit
TAGCGAAGTTCCTGATTCCCTGTAGGCACCATCAATAGATCGGAAGAGCA296860.11224743712209459No Hit
AACACCCGAAGTCGGTGAGGTAACCTTCTGTAGGCACCATCAATAGATCG295080.11157439111361474No Hit
AAGGCTGAGCTGTGATGGCGAGCGAAATATAGTCTGTAGGCACCATCAAT294550.1113739897740112No Hit
ACCGATATGCTTCGGGGAGCTGTAAGCCTGTAGGCACCATCAATAGATCG293770.11107905950063239No Hit
ACAGGTAGGCGAGGAGAGAATCCTCTGTAGGCACCATCAATAGATCGGAA292750.11069338145082931No Hit
ATATCTGAATTCATAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCA289440.10944181836764488No Hit
AAGCACTCCGCCTGGGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGC288460.10907126494724587No Hit
ACACCACGAGAGTTTGTAACCTGTAGGCACCATCAATAGATCGGAAGAGC284820.10769492367147808No Hit
ACGTGAAATTCCGTCGGAATCCTGTAGGCACCATCAATAGATCGGAAGAG284320.10750586580392754No Hit
ATGAAGCCCCCCTCAAGATGAGATTTCCCCTGTAGGCACCATCAATAGAT283150.10706347039385934No Hit
ACCTCCTCACCATTTGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA282300.10674207201902346No Hit
AAACCAAGAGGCTTGCCTCTTGGGGTTGTCTGTAGGCACCATCAATAGAT279410.10564931754458146No Hit
AGACCCGGGGAACTGAAACCTGTAGGCACCATCAATAGATCGGAAGAGCA279100.10553210166670013No Hit
ATCTAGTGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA277760.10502542658166474Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGCTGTGATGGCGAGCGAACTGTAGGCACCATCAATAGATCGG277520.10493467880524049No Hit
ACGGCCCAGACTCCTACGGGAGGCAGCAGTCTGTAGGCACCATCAATAGA274970.10397048368073283No Hit
ACTCCCTAGTGACCGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA271990.10284369879013174No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCCTGTAGGCACCATCAATAGA265530.10040107114137901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)